(Circular) (Six-base) MAPSORT of: pFN21AE9447.seq Check: 2640 from: 1 to: 5911 pFN21AE9447 5911 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2913 3254 1937 Size: 976 341 4594 Fragments arranged by size: 4594 976 341 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 2419 4038 4411 1563 Size: 856 1619 373 3063 Fragments arranged by size: 3063 1619 856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2095 2702 3974 5358 5626 1632 Size: 463 607 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1272 607 463 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 2503 1166 Size: 1337 4574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2198 1184 Size: 1014 4897 AlwNI CAG_nnn'CTG Cuts at: 2974 3026 3654 5226 2974 Size: 52 628 1572 3659 Fragments arranged by size: 3659 1572 628 52 ApaLI G'TGCA_C Cuts at: 3968 5124 3968 Size: 1156 4755 ApoI r'AATT_y Cuts at: 1493 3220 3446 3598 5707 1493 Size: 1727 226 152 2109 1697 Fragments arranged by size: 2109 1727 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2011 3236 1870 Size: 73 68 1225 4545 Fragments arranged by size: 4545 1225 73 68 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2120 2641 2994 3232 Size: 325 217 627 290 43 521 353 238 Cuts at: 3232 4687 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 521 353 325 290 238 217 43 BanII G_rGCy'C Cuts at: 1391 2099 2153 3230 1391 Size: 708 54 1077 4072 Fragments arranged by size: 4072 1077 708 54 BbeI G_GCGC'C Cuts at: 2081 2081 Size: 5911 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2926 928 Size: 971 1027 3913 Fragments arranged by size: 3913 1027 971 BbvCI CC'TCA_GC Cuts at: 2067 2258 2990 2067 Size: 191 732 4988 Fragments arranged by size: 4988 732 191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2356 2390 4102 4136 1903 Size: 34 419 34 1712 34 3678 Fragments arranged by size: 3678 1712 419 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 2448 2733 2940 1548 Size: 900 285 207 4519 Fragments arranged by size: 4519 900 285 207 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2471 4522 136 Size: 107 122 71 631 605 799 2051 1525 Fragments arranged by size: 2051 1525 799 631 605 122 107 71 BglII A'GATC_T Cuts at: 2713 5906 2713 Size: 3193 2718 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2646 3972 5128 1790 Size: 856 1326 1156 2573 Fragments arranged by size: 2573 1326 1156 856 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2050 3642 4600 Size: 416 53 83 399 24 633 1592 958 Cuts at: 4600 442 Size: 1753 Fragments arranged by size: 1753 1592 958 633 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2537 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2488 4571 1239 Size: 1249 2083 2579 Fragments arranged by size: 2579 2083 1249 Bpu10I CC'TnA_GC Cuts at: 2067 2258 2990 2067 Size: 191 732 4988 Fragments arranged by size: 4988 732 191 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4574 882 Size: 617 3075 2219 Fragments arranged by size: 3075 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3702 493 Size: 711 2498 2702 Fragments arranged by size: 2702 2498 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2169 3718 4100 Size: 53 83 186 936 545 91 1549 382 Cuts at: 4100 4735 275 Size: 635 1451 Fragments arranged by size: 1549 1451 936 635 545 382 186 91 83 53 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2588 2618 1178 Size: 30 165 30 1185 30 4471 Fragments arranged by size: 4471 1185 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 1186 Size: 1042 4869 BseYI C'CCAG_C Cuts at: 2146 2164 2322 5114 5610 2146 Size: 18 158 2792 496 2447 Fragments arranged by size: 2792 2447 496 158 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2551 1163 Size: 573 815 4523 Fragments arranged by size: 4523 815 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4122 5150 1540 Size: 275 186 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2518 3972 4057 5128 2193 Size: 325 1454 85 1071 2976 Fragments arranged by size: 2976 1454 1071 325 85 BsmI GAATG_Cn' Cuts at: 2700 3422 3515 2700 Size: 722 93 5096 Fragments arranged by size: 5096 722 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2153 2193 2518 2646 3230 3972 Size: 399 309 54 40 325 128 584 742 Cuts at: 3972 4057 5128 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 584 399 325 309 128 85 54 40 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 2937 3800 5530 2937 Size: 863 1730 3318 Fragments arranged by size: 3318 1730 863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2190 2274 3266 844 Size: 1346 84 992 3489 Fragments arranged by size: 3489 1346 992 84 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2388 2662 4406 4580 65 Size: 1183 1140 274 1744 174 1396 Fragments arranged by size: 1744 1396 1183 1140 274 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 2080 4555 4742 1700 Size: 110 264 6 2475 187 2869 Fragments arranged by size: 2869 2475 264 187 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5911 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5911 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5911 BstBI TT'CG_AA Cuts at: 2692 2953 4715 2692 Size: 261 1762 3888 Fragments arranged by size: 3888 1762 261 BstEII G'GTnAC_C Cuts at: 1184 2287 2852 1184 Size: 1103 565 4243 Fragments arranged by size: 4243 1103 565 BstXI CCAn_nnnn'nTGG Cuts at: 2669 2669 Size: 5911 BstYI r'GATC_y Cuts at: 1972 2713 2957 3241 3608 3993 4010 5462 5548 Size: 741 244 284 367 385 17 1452 86 Cuts at: 5548 5560 5906 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 741 385 367 346 284 244 86 17 12 BstZ17I GTA'TAC Cuts at: 2914 2914 Size: 5911 Bsu36I CC'TnA_GG Cuts at: 2312 2312 Size: 5911 BtgI C'CryG_G Cuts at: 513 1065 2032 2058 2337 513 Size: 552 967 26 279 4087 Fragments arranged by size: 4087 967 552 279 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 3427 4221 4241 3427 Size: 794 20 5097 Fragments arranged by size: 5097 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 2745 3215 2745 Size: 470 5441 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2671 2763 Size: 54 1425 50 275 18 199 642 92 Cuts at: 2763 3358 4247 5876 8 Size: 595 889 1629 43 Fragments arranged by size: 1629 1425 889 642 595 275 199 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2285 2378 3112 3841 1353 Size: 761 171 93 734 729 3423 Fragments arranged by size: 3423 761 734 729 171 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2488 2702 3974 4571 5358 5626 Size: 393 463 393 214 1272 597 787 268 Cuts at: 5626 1239 Size: 1524 Fragments arranged by size: 1524 1272 787 597 463 393 393 268 214 EcoNI CCTnn'n_nnAGG Cuts at: 2311 2311 Size: 5911 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 2067 2081 2134 2141 5058 1678 Size: 389 14 53 7 2917 2531 Fragments arranged by size: 2917 2531 389 53 14 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2567 2599 4552 4584 4626 Size: 32 135 32 995 32 1953 32 42 Cuts at: 4626 4658 1373 Size: 32 2626 Fragments arranged by size: 2626 1953 995 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2248 3154 3255 3501 669 Size: 988 281 310 906 101 246 3079 Fragments arranged by size: 3079 988 906 310 281 246 101 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KasI G'GCGC_C Cuts at: 2077 2077 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2786 2891 3007 5025 5209 1305 Size: 1481 105 116 2018 184 2007 Fragments arranged by size: 2018 2007 1481 184 116 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 2673 2765 10 Size: 54 1425 343 841 92 3156 Fragments arranged by size: 3156 1425 841 343 92 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 2503 2667 2693 3056 3869 4228 Size: 648 1263 74 164 26 363 813 359 Cuts at: 4228 4387 518 Size: 159 2042 Fragments arranged by size: 2042 1263 813 648 363 359 164 159 74 26 MspA1I CmG'CkG Cuts at: 1606 2025 2060 2215 3026 3327 4004 5152 5397 Size: 419 35 155 811 301 677 1148 245 Cuts at: 5397 1606 Size: 2120 Fragments arranged by size: 2120 1148 811 677 419 301 245 155 35 NaeI GCC'GGC Cuts at: 2076 2082 2076 Size: 6 5905 NarI GG'CG_CC Cuts at: 2078 2078 Size: 5911 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NgoMIV G'CCGG_C Cuts at: 2074 2080 2074 Size: 6 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 3269 4814 1132 Size: 97 2040 1545 2229 Fragments arranged by size: 2229 2040 1545 97 PasI CC'CwG_GG Cuts at: 1348 2647 1348 Size: 1299 4612 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 3026 1108 Size: 1918 3993 PfoI T'CCnGG_A Cuts at: 2544 2544 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2897 2929 3183 3215 2897 Size: 32 254 32 5593 Fragments arranged by size: 5593 254 32 32 PstI C_TGCA'G Cuts at: 830 3263 830 Size: 2433 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 3026 3026 Size: 5911 SacII CC_GC'GG Cuts at: 2061 2061 Size: 5911 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SexAI A'CCwGG_T Cuts at: 2856 2856 Size: 5911 SfcI C'TryA_G Cuts at: 826 1045 3259 4394 5075 5266 826 Size: 219 2214 1135 681 191 1471 Fragments arranged by size: 2214 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2471 2471 Size: 5911 SfoI GGC'GCC Cuts at: 2079 2079 Size: 5911 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 2080 1700 Size: 380 5531 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 3004 1517 Size: 1487 4424 StyI C'CwwG_G Cuts at: 513 1065 2586 3350 513 Size: 552 1521 764 3074 Fragments arranged by size: 3074 1521 764 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2314 2639 3938 3955 4108 4293 4738 Size: 1255 734 325 1299 17 153 185 445 Cuts at: 4738 325 Size: 1498 Fragments arranged by size: 1498 1299 1255 734 445 325 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 4157 5869 Size: 274 80 33 51 1443 30 2150 1712 Cuts at: 5869 96 Size: 138 Fragments arranged by size: 2150 1712 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2161 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 2715 4040 2715 Size: 1325 4586 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SapI SrfI SwaI