################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eco47III Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MflI MluI MroI MslI MvnI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AhdI 1573 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1003 GGGCCC GGGCC^C AspEI 1573 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1726 GAGCTC G[AT]GC[AT]^C BalI 1355 TGGCCA TGG^CCA BanI 69 GGTGCC G^G[CT][GA]CC BciVI 1224 GGATAC GTATCC(6/5) BcnI 1034 CCGGG CC^[GC]GG BglI 1348 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 262 GGTCTC GGTCTC(1/5) BsgI 968 CTGCAC GTGCAG(16/14) BsiEI 890 CGGCCG CG[GA][CT]^CG BsiHKAI 1726 GAGCTC G[AT]GC[AT]^C BsmFI 924 GGGAC GGGAC(10/14) BsmI 1248 GAATGC GAATGC(1/-1) BspMI 381 ACCTGC ACCTGC(4/8) BsrDI 356 CATTGC GCAATG(2/0) BsrFI 888 GCCGGC [GA]^CCGG[CT] BstAPI 25 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 783 GCGC GCG^C Cfr10I 888 GCCGGC [GA]^CCGG[CT] DrdI 163 GACCATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1293 CCGTGG C^C[GA][CT]GG EagI 890 CGGCCG C^GGCCG Eam1105I 1573 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 890 CGGCCG C^GGCCG Eco52I 890 CGGCCG C^GGCCG EcoNI 994 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 313 ATGCAT ATGCA^T FauI 471 GCGGG CCCGC(4/6) HhaI 783 GCGC GCG^C HinP1I 783 GCGC G^CGC MfeI 1184 CAATTG C^AATTG MluNI 1355 TGGCCA TGG^CCA MscI 1355 TGGCCA TGG^CCA MunI 1184 CAATTG C^AATTG NaeI 888 GCCGGC GCC^GGC NciI 1034 CCGGG CC^[GC]GG NgoMIV 888 GCCGGC G^CCGGC NsiI 313 ATGCAT ATGCA^T NspI 315 GCATGC [GA]CATG^[CT] PflMI 73 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1579 AGGTCCT [GA]G^G[AT]CC[CT] SacI 1726 GAGCTC GAGCT^C SapI 1723 GAAGAGC GCTCTTC(1/4) SmlI 386 CTCAAG C^T[CT][GA]AG SphI 315 GCATGC GCATG^C StuI 1448 AGGCCT AGG^CCT Van91I 73 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 333 AAATTC [GA]^AATT[CT] 422 AAATTC AlwI 666 GGATC GGATC(4/5) 1384 GGATC ApoI 333 AAATTC [GA]^AATT[CT] 422 AAATTC BanII 1003 GGGCCC G[GA]GC[CT]^C 1726 GAGCTC BfaI 248 CTAG C^TAG 362 CTAG DpnII 667 GATC ^GATC 1385 GATC EaeI 890 CGGCCG [CT]^GGCC[GA] 1355 TGGCCA EcoT14I 1486 CCTTGG C^C[AT][AT]GG 1549 CCAAGG MaeI 248 CTAG C^TAG 362 CTAG MboI 667 GATC ^GATC 1385 GATC NdeII 667 GATC ^GATC 1385 GATC Sau3AI 667 GATC ^GATC 1385 GATC StyI 1486 CCTTGG C^C[AT][AT]GG 1549 CCAAGG Tsp45I 1212 GTCAC ^GT[GC]AC 1521 GTCAC TspRI 235 CAGTG CA[GC]TG[ACGT][ACGT]^ 1629 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NlaIII NlaIV PleI PvuII RsaI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I