(Linear) (Six-base) MAPSORT of: KIEE2520.seq Check: 5417 from: 1 to: 1773 KIEE2520.orf With 182 enzymes: SgfI * March 20, 2014 14:15 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 962 1693 1773 Size: 962 731 80 Fragments arranged by size: 962 731 80 AhdI GACnn_n'nnGTC Cuts at: 0 1578 1773 Size: 1578 195 AlwNI CAG_nnn'CTG Cuts at: 0 967 1435 1752 1773 Size: 967 468 317 21 Fragments arranged by size: 967 468 317 21 ApaI G_GGCC'C Cuts at: 0 1007 1773 Size: 1007 766 ApoI r'AATT_y Cuts at: 0 333 422 1773 Size: 333 89 1351 Fragments arranged by size: 1351 333 89 BanI G'GyrC_C Cuts at: 0 69 1773 Size: 69 1704 BanII G_rGCy'C Cuts at: 0 1007 1730 1773 Size: 1007 723 43 Fragments arranged by size: 1007 723 43 BbsI GAAGACnn'nnnn_ Cuts at: 0 288 438 1157 1773 Size: 288 150 719 616 Fragments arranged by size: 719 616 288 150 BciVI GTATCCnnnnn_n' Cuts at: 0 1218 1773 Size: 1218 555 BfrBI ATG'CAT Cuts at: 0 315 1773 Size: 315 1458 BglI GCCn_nnn'nGGC Cuts at: 0 1354 1773 Size: 1354 419 Bme1580I G_kGCm'C Cuts at: 0 1007 1350 1773 Size: 1007 343 423 Fragments arranged by size: 1007 423 343 BmrI ACTGGGnnnn_n' Cuts at: 0 1622 1773 Size: 1622 151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1245 1773 Size: 1245 528 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 502 562 592 631 649 667 724 Size: 487 15 60 30 39 18 18 57 Cuts at: 724 1518 1773 Size: 794 255 Fragments arranged by size: 794 487 255 60 57 39 30 18 18 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 371 1773 Size: 371 1402 BsaI GGTCTCn'nnnn_ Cuts at: 0 268 1773 Size: 268 1505 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1023 1053 1773 Size: 1023 30 720 Fragments arranged by size: 1023 720 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 815 935 983 1007 1043 1052 1076 1079 Size: 815 120 48 24 36 9 24 3 Cuts at: 1079 1377 1773 Size: 298 396 Fragments arranged by size: 815 396 298 120 48 36 24 24 9 3 BseYI C'CCAG_C Cuts at: 0 840 1773 Size: 840 933 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 953 1773 Size: 953 820 BsiEI CG_ry'CG Cuts at: 0 893 1773 Size: 893 880 BsiHKAI G_wGCw'C Cuts at: 0 1730 1773 Size: 1730 43 BsmI GAATG_Cn' Cuts at: 0 1254 1773 Size: 1254 519 Bsp1286I G_dGCh'C Cuts at: 0 1007 1350 1730 1773 Size: 1007 343 380 43 Fragments arranged by size: 1007 380 343 43 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 390 1773 Size: 390 1383 BsrBI CCG'CTC Cuts at: 0 471 615 633 651 1773 Size: 471 144 18 18 1122 Fragments arranged by size: 1122 471 144 18 18 BsrDI GCAATG_nn' Cuts at: 0 355 1773 Size: 355 1418 BsrFI r'CCGG_y Cuts at: 0 888 1773 Size: 888 885 BstAPI GCAn_nnn'nTGC Cuts at: 0 31 1773 Size: 31 1742 BtgI C'CryG_G Cuts at: 0 1293 1773 Size: 1293 480 DrdI GACnn_nn'nnGTC Cuts at: 0 169 1773 Size: 169 1604 EaeI y'GGCC_r Cuts at: 0 890 1355 1773 Size: 890 465 418 Fragments arranged by size: 890 465 418 EagI C'GGCC_G Cuts at: 0 890 1773 Size: 890 883 EarI CTCTTCn'nnn_ Cuts at: 0 855 1458 1718 1773 Size: 855 603 260 55 Fragments arranged by size: 855 603 260 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1257 1773 Size: 1257 516 EcoICRI GAG'CTC Cuts at: 0 1728 1773 Size: 1728 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 502 562 592 631 649 667 724 Size: 487 15 60 30 39 18 18 57 Cuts at: 724 962 1518 1693 1773 Size: 238 556 175 80 Fragments arranged by size: 556 487 238 175 80 60 57 39 30 18 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 998 1773 Size: 998 775 EcoO109I rG'GnC_Cy Cuts at: 0 964 1003 1004 1580 1773 Size: 964 39 1 576 193 Fragments arranged by size: 964 576 193 39 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1502 1773 Size: 1502 271 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 3 35 92 124 886 918 1564 1596 Size: 3 32 57 32 762 32 646 32 Cuts at: 1596 1773 Size: 177 Fragments arranged by size: 762 646 177 57 32 32 32 32 3 MfeI C'AATT_G Cuts at: 0 1184 1773 Size: 1184 589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 491 1773 Size: 491 1282 MscI TGG'CCA Cuts at: 0 1357 1773 Size: 1357 416 MspA1I CmG'CkG Cuts at: 0 76 570 765 1180 1773 Size: 76 494 195 415 593 Fragments arranged by size: 593 494 415 195 76 NaeI GCC'GGC Cuts at: 0 890 1773 Size: 890 883 NgoMIV G'CCGG_C Cuts at: 0 888 1773 Size: 888 885 NsiI A_TGCA'T Cuts at: 0 317 1773 Size: 317 1456 NspI r_CATG'y Cuts at: 0 319 1773 Size: 319 1454 PasI CC'CwG_GG Cuts at: 0 1000 1773 Size: 1000 773 PflMI CCAn_nnn'nTGG Cuts at: 0 79 1773 Size: 79 1694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1255 1287 1773 Size: 1255 32 486 Fragments arranged by size: 1255 486 32 PpuMI rG'GwC_Cy Cuts at: 0 1580 1773 Size: 1580 193 PspOMI G'GGCC_C Cuts at: 0 1003 1773 Size: 1003 770 PvuII CAG'CTG Cuts at: 0 76 570 765 1180 1773 Size: 76 494 195 415 593 Fragments arranged by size: 593 494 415 195 76 SacI G_AGCT'C Cuts at: 0 1730 1773 Size: 1730 43 SapI GCTCTTCn'nnn_ Cuts at: 0 1718 1773 Size: 1718 55 SmlI C'TyrA_G Cuts at: 0 386 1773 Size: 386 1387 SphI G_CATG'C Cuts at: 0 319 1773 Size: 319 1454 StuI AGG'CCT Cuts at: 0 1450 1773 Size: 1450 323 StyI C'CwwG_G Cuts at: 0 1486 1549 1773 Size: 1486 63 224 Fragments arranged by size: 1486 224 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 57 1773 Size: 57 1716 TatI w'GTAC_w Cuts at: 0 206 1462 1773 Size: 206 1256 311 Fragments arranged by size: 1256 311 206 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 212 244 1023 1055 1773 Size: 212 32 779 32 718 Fragments arranged by size: 779 718 212 32 32 Enzymes that do cut: AcuI AhdI AlwNI ApaI ApoI BanI BanII BbsI BciVI BfrBI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BtgI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MfeI MmeI MscI MspA1I NaeI NgoMIV NsiI NspI PasI PflMI PpiI PpuMI PspOMI PvuII SacI SapI SmlI SphI StuI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MslI NarI NcoI NdeI NheI NotI NruI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI