(Circular) (Six-base) MAPSORT of: pFN21AE1903.seq Check: 3456 from: 1 to: 6004 pFN21AE1903 6004 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3239 4067 5451 5719 1632 Size: 1607 828 1384 268 1917 Fragments arranged by size: 1917 1607 1384 828 268 AfeI AGC'GCT Cuts at: 2036 2036 Size: 6004 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 3012 3018 4903 5880 1203 Size: 1809 6 1885 977 1327 Fragments arranged by size: 1885 1809 1327 977 6 AgeI A'CCGG_T Cuts at: 2760 4835 2760 Size: 2075 3929 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2239 1184 Size: 1055 4949 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 2087 Size: 32 5972 AlwNI CAG_nnn'CTG Cuts at: 3747 5319 3747 Size: 1572 4432 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2498 3313 3539 3691 5800 1493 Size: 1005 815 226 152 2109 1697 Fragments arranged by size: 2109 1697 1005 815 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2047 3329 1870 Size: 73 104 1282 4545 Fragments arranged by size: 4545 1282 104 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3325 4780 618 Size: 325 217 627 1538 1455 1842 Fragments arranged by size: 1842 1538 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2074 2973 2993 3082 3323 1391 Size: 683 899 20 89 241 4072 Fragments arranged by size: 4072 899 683 241 89 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2025 4615 Size: 107 122 71 631 605 337 16 2590 Cuts at: 4615 136 Size: 1525 Fragments arranged by size: 2590 1525 631 605 337 122 107 71 16 BglII A'GATC_T Cuts at: 2961 5999 2961 Size: 3038 2966 BlpI GC'TnA_GC Cuts at: 2849 2972 3421 2849 Size: 123 449 5432 Fragments arranged by size: 5432 449 123 Bme1580I G_kGCm'C Cuts at: 1790 4065 5221 1790 Size: 2275 1156 2573 Fragments arranged by size: 2573 2275 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3079 3735 4693 Size: 416 53 83 399 24 1662 656 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1662 958 656 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2978 4664 1239 Size: 812 927 1686 2579 Fragments arranged by size: 2579 1686 927 812 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4667 882 Size: 617 3168 2219 Fragments arranged by size: 3168 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2128 3795 493 Size: 711 924 1667 2702 Fragments arranged by size: 2702 1667 924 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2760 2841 2919 4437 4835 5109 5256 5667 2760 Size: 81 78 1518 398 274 147 411 3097 Fragments arranged by size: 3097 1518 411 398 274 147 81 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2468 2498 2694 2724 3134 Size: 30 165 30 1065 30 196 30 410 Cuts at: 3134 3164 1178 Size: 30 4018 Fragments arranged by size: 4018 1065 410 196 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 1186 Size: 1534 4470 BseYI C'CCAG_C Cuts at: 5207 5703 5207 Size: 496 5508 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5431 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2973 2993 4065 4150 5221 2133 Size: 840 20 1072 85 1071 2916 Fragments arranged by size: 2916 1072 1071 840 85 20 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2133 2973 2993 3082 3323 4065 Size: 399 284 59 840 20 89 241 742 Cuts at: 4065 4150 5221 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 840 742 399 284 241 89 85 59 20 BspEI T'CCGG_A Cuts at: 2841 2919 5667 2841 Size: 78 2748 3178 Fragments arranged by size: 3178 2748 78 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3359 844 Size: 2515 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2146 3891 1394 Size: 429 323 1745 3507 Fragments arranged by size: 3507 1745 429 323 BsrDI GCAATG_nn' Cuts at: 65 1248 2233 3228 4499 4673 65 Size: 1183 985 995 1271 174 1396 Fragments arranged by size: 1396 1271 1183 995 985 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 4648 4835 1700 Size: 110 950 1888 187 2869 Fragments arranged by size: 2869 1888 950 187 110 BsrGI T'GTAC_A Cuts at: 96 2683 3015 3057 96 Size: 2587 332 42 3043 Fragments arranged by size: 3043 2587 332 42 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2157 794 Size: 1363 4641 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstYI r'GATC_y Cuts at: 1972 2961 3334 3701 4086 4103 5555 5641 5653 Size: 989 373 367 385 17 1452 86 12 Cuts at: 5653 5999 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 989 385 373 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2280 3042 513 Size: 552 1215 762 3475 Fragments arranged by size: 3475 1215 762 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2155 3520 4314 4334 2155 Size: 1365 794 20 3825 Fragments arranged by size: 3825 1365 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 3451 4340 Size: 54 1425 50 275 18 513 1108 889 Cuts at: 4340 5969 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1108 889 513 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2907 3934 1353 Size: 1554 1027 3423 Fragments arranged by size: 3423 1554 1027 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2971 2991 2971 Size: 20 5984 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2978 3239 4067 4664 5451 5719 Size: 393 419 927 261 828 597 787 268 Cuts at: 5719 1239 Size: 1524 Fragments arranged by size: 1524 927 828 787 597 419 393 268 261 EcoNI CCTnn'n_nnAGG Cuts at: 3221 3221 Size: 6004 EcoO109I rG'GnC_Cy Cuts at: 2017 2661 2017 Size: 644 5360 EcoRI G'AATT_C Cuts at: 2498 3313 3691 2498 Size: 815 378 4811 Fragments arranged by size: 4811 815 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2790 2790 Size: 6004 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 2038 5151 1678 Size: 360 3113 2531 Fragments arranged by size: 3113 2531 360 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2393 2425 2871 2903 3084 Size: 32 135 32 821 32 446 32 181 Cuts at: 3084 3116 4645 4677 4719 4751 1373 Size: 32 1529 32 42 32 2626 Fragments arranged by size: 2626 1529 821 446 181 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2140 2542 3348 3594 669 Size: 988 281 202 402 806 246 3079 Fragments arranged by size: 3079 988 806 402 281 246 202 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 2542 3594 2542 Size: 1052 4952 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 2559 3603 2559 Size: 1044 4960 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5118 5302 1305 Size: 3813 184 2007 Fragments arranged by size: 3813 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2345 10 Size: 54 1425 343 513 3669 Fragments arranged by size: 3669 1425 513 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3962 4321 4480 518 Size: 648 2796 359 159 2042 Fragments arranged by size: 2796 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3420 4097 5245 5490 1606 Size: 1814 677 1148 245 2120 Fragments arranged by size: 2120 1814 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2280 3042 513 Size: 552 1215 762 3475 Fragments arranged by size: 3475 1215 762 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 3022 3064 3362 4907 1132 Size: 97 1793 42 298 1545 2229 Fragments arranged by size: 2229 1793 1545 298 97 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 3018 4903 3018 Size: 1885 4119 PflMI CCAn_nnn'nTGG Cuts at: 1108 3043 1108 Size: 1935 4069 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2661 2661 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PstI C_TGCA'G Cuts at: 830 2045 2079 3356 830 Size: 1215 34 1277 3478 Fragments arranged by size: 3478 1277 1215 34 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 SacI G_AGCT'C Cuts at: 2973 2993 2973 Size: 20 5984 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SanDI GG'GwC_CC Cuts at: 2661 2661 Size: 6004 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 2165 2526 4252 5964 2165 Size: 361 1726 1712 2205 Fragments arranged by size: 2205 1726 1712 361 SfcI C'TryA_G Cuts at: 826 1045 2041 2075 3112 3352 4487 5168 5359 Size: 219 996 34 1037 240 1135 681 191 Cuts at: 5359 826 Size: 1471 Fragments arranged by size: 1471 1135 1037 996 681 240 219 191 34 SfiI GGCCn_nnn'nGGCC Cuts at: 2025 2025 Size: 6004 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 2049 3331 1872 Size: 177 1282 4545 Fragments arranged by size: 4545 1282 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SrfI GCCC'GGGC Cuts at: 2049 2049 Size: 6004 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2280 2811 2829 3042 3443 513 Size: 552 1215 531 18 213 401 3074 Fragments arranged by size: 3074 1215 552 531 401 213 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2376 3035 4031 4048 4201 4386 4831 Size: 1255 796 659 996 17 153 185 445 Cuts at: 4831 325 Size: 1498 Fragments arranged by size: 1498 1255 996 796 659 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 2524 2683 Size: 274 80 33 51 1443 186 361 159 Cuts at: 2683 3015 3057 3132 4250 5962 96 Size: 332 42 75 1118 1712 138 Fragments arranged by size: 1712 1443 1118 361 332 274 186 159 138 80 75 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 2924 2956 5964 5996 2468 Size: 32 424 32 3008 32 2476 Fragments arranged by size: 3008 2476 424 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2885 1316 Size: 1569 4435 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2881 3235 2263 Size: 618 354 5032 Fragments arranged by size: 5032 618 354 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 2047 3329 1870 Size: 177 1282 4545 Fragments arranged by size: 4545 1282 177 XmnI GAAnn'nnTTC Cuts at: 2374 4133 2374 Size: 1759 4245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfoI SwaI