Restriction map for pFN21AE2512
 (Circular) (Six-base) MAPSORT of: pFN21AE2512.seq  Check: 4742  from: 1  to: 5569



pFN21AE2512  5569 bp

 With 182 enzymes: SgfI * 

                        February 25, 2014 17:19  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2395    3379     278 
   Size:         53      83     186     936     859     984    2468

  Fragments arranged by size: 

                2468     984     936     859     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2912    1937 
   Size:        975    4594
 
Acc65I G'GTAC_C

Cuts at:   2890    2890 
   Size:       5569
 
AclI AA'CG_TT

Cuts at:   1563    3696    4069    1563 
   Size:       2133     373    3063

  Fragments arranged by size: 

                3063    2133     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2121    2190    2484    3632    5016    5284    1632 
   Size:        489      69     294    1148    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1148     489     294     268      69
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5372
 
AflIII A'CryG_T

Cuts at:   1203    4468    5445    1203 
   Size:       3265     977    1327

  Fragments arranged by size: 

                3265    1327     977
 
AgeI A'CCGG_T

Cuts at:   4400    4400 
   Size:       5569
 
AhdI GACnn_n'nnGTC

Cuts at:   4298    4298 
   Size:       5569
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5569
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5569
 
AlwNI CAG_nnn'CTG

Cuts at:   2111    2317    3312    4884    2111 
   Size:        206     995    1572    2796

  Fragments arranged by size: 

                2796    1572     995     206
 
ApaLI G'TGCA_C

Cuts at:   3626    4782    3626 
   Size:       1156    4413
 
ApoI r'AATT_y

Cuts at:   1493    2878    3104    3256    5365    1493 
   Size:       1385     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1385     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4123     160 
   Size:        872    3091    1606

  Fragments arranged by size: 

                3091    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5569
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2894    1870 
   Size:         73     951    4545

  Fragments arranged by size: 

                4545     951      73
 
BamHI G'GATC_C

Cuts at:   2899    3266    2899 
   Size:        367    5202
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2227    2890    4345     618 
   Size:        325     217     627     440     663    1455    1842

  Fragments arranged by size: 

                1842    1455     663     627     440     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2364    2476    2888    1391 
   Size:        973     112     412    4072

  Fragments arranged by size: 

                4072     973     412     112
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4598
 
BbvCI CC'TCA_GC

Cuts at:   2508    2690    2508 
   Size:        182    5387
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3760    3794    1903 
   Size:         34    1823      34    3678

  Fragments arranged by size: 

                3678    1823      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3461    4671    3461 
   Size:       1210    4359
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5569
 
BfrBI ATG'CAT

Cuts at:   2551    2551 
   Size:       5569
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4180     136 
   Size:        107     122      71     631     605    2508    1525

  Fragments arranged by size: 

                2508    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5564    5564 
   Size:       5569
 
BlpI GC'TnA_GC

Cuts at:   2986    2986 
   Size:       5569
 
Bme1580I G_kGCm'C

Cuts at:   1790    3630    4786    1790 
   Size:       1840    1156    2573

  Fragments arranged by size: 

                2573    1840    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5569
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3300    4258     442 
   Size:        416      53      83     399      24    1883     958    1753

  Fragments arranged by size: 

                1883    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5569
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4229    1239 
   Size:       2990    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2508    2690    2508 
   Size:        182    5387
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3694    4559    4857    3694 
   Size:        865     298    4406

  Fragments arranged by size: 

                4406     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2108    2117    4232     882 
   Size:        617     609       9    2115    2219

  Fragments arranged by size: 

                2219    2115     617     609       9
 
BsaAI yAC'GTr

Cuts at:    493    1204    3360     493 
   Size:        711    2156    2702

  Fragments arranged by size: 

                2702    2156     711
 
BsaBI GATnn'nnATC

Cuts at:   5563    5563 
   Size:       5569
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2392    3376    3758    4393 
   Size:         53      83     186     936     859     984     382     635

Cuts at:   4393     275 
   Size:       1451

  Fragments arranged by size: 

                1451     984     936     859     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2277    2424    4002    4400    4674    4821    5232    2277 
   Size:        147    1578     398     274     147     411    2614

  Fragments arranged by size: 

                2614    1578     411     398     274     147     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2241    2271    1178 
   Size:         30     165      30     838      30    4476

  Fragments arranged by size: 

                4476     838     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2054    2057    2060    2093    2135    2201    2366    2369 
   Size:        868       3       3      33      42      66     165       3

Cuts at:   2369    2372    2498    2501    2504    2662    1186 
   Size:          3     126       3       3     158    4093

  Fragments arranged by size: 

                4093     868     165     158     126      66      42      33
                   3       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:   4772    5268    4772 
   Size:        496    5073
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4996
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3019    3780    4808    1540 
   Size:        275     186    1018     761    1028    2301

  Fragments arranged by size: 

                2301    1028    1018     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2364    3630    3715    4786    2364 
   Size:       1266      85    1071    3147

  Fragments arranged by size: 

                3147    1266    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3080    3173    3080 
   Size:         93    5476
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2383    2383 
   Size:       5569
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2364    2476    2888    3630    3715    4786    1391 
   Size:        399     574     112     412     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     574     412     399     112      85
            
 
BspEI T'CCGG_A

Cuts at:   2424    5232    2424 
   Size:       2808    2761
 
BspHI T'CATG_A

Cuts at:   3458    5188    3458 
   Size:       1730    3839
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2760    2924     844 
   Size:       1916     164    3489

  Fragments arranged by size: 

                3489    1916     164
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2404    2434    2446    2590    2617    3456    1394 
   Size:        429     581      30      12     144      27     839    3507

  Fragments arranged by size: 

                3507     839     581     429     144      30      27      12
            
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4064    4238      65 
   Size:       1183    2816     174    1396

  Fragments arranged by size: 

                2816    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4213    4400    1700 
   Size:        110    2403     187    2869

  Fragments arranged by size: 

                2869    2403     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5569
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5569
 
BssSI C'ACGA_G

Cuts at:   3629    4641    3629 
   Size:       1012    4557
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5569
 
BstBI TT'CG_AA

Cuts at:   4373    4373 
   Size:       5569
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5569
 
BstYI r'GATC_y

Cuts at:   1972    2238    2899    3266    3651    3668    5120    5206    5218 
   Size:        266     661     367     385      17    1452      86      12

Cuts at:   5218    5564    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     661     385     367     346     266      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2836     513 
   Size:        552    1771    3246

  Fragments arranged by size: 

                3246    1771     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4830

  Fragments arranged by size: 

                4830     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3085    3879    3899    3085 
   Size:        794      20    4755

  Fragments arranged by size: 

                4755     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5569
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5534
 
DraI TTT'AAA

Cuts at:   2873    2873 
   Size:       5569
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4576     809 
   Size:        507    3260    1802

  Fragments arranged by size: 

                3260    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2661    3016    3905 
   Size:         54    1425      50     275      18     831     355     889

Cuts at:   3905    5534       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     831     355     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3016    1537 
   Size:        275    1204    4090

  Fragments arranged by size: 

                4090    1204     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2013    2025    2064    2085    2145    2460    2597    3499 
   Size:        660      12      39      21      60     315     137     902

Cuts at:   3499    1353 
   Size:       3423

  Fragments arranged by size: 

                3423     902     660     315     137      60      39      21
                  12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4156    4530    4676     387 
   Size:       1064     138    2567     374     146    1280

  Fragments arranged by size: 

                2567    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2362    2362 
   Size:       5569
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2121    2190    2484    3632    4229    5016    5284 
   Size:        393     489      69     294    1148     597     787     268

Cuts at:   5284    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1148     787     597     489     393     294     268
                  69
 
EcoO109I rG'GnC_Cy

Cuts at:   2296    2296 
   Size:       5569
 
EcoRI G'AATT_C

Cuts at:   2878    3256    2878 
   Size:        378    5191
 
FspI TGC'GCA

Cuts at:   4075    4075 
   Size:       5569
 
HaeII r_GCGC'y

Cuts at:   1678    2318    2863    4716    1678 
   Size:        640     545    1853    2531

  Fragments arranged by size: 

                2531    1853     640     545
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2311    2343    4210    4242    4284 
   Size:         32     135      32     739      32    1867      32      42

Cuts at:   4284    4316    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1867     739     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2913    3159     669 
   Size:        988     281     975     246    3079

  Fragments arranged by size: 

                3079     988     975     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5569
 
HpaI GTT'AAC

Cuts at:   3159    3159 
   Size:       5569
 
KpnI G_GTAC'C

Cuts at:   2894    2894 
   Size:       5569
 
MfeI C'AATT_G

Cuts at:   3168    3168 
   Size:       5569
 
MluI A'CGCG_T

Cuts at:   5445    5445 
   Size:       5569
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2565    4683    4867    1305 
   Size:       1260    2118     184    2007

  Fragments arranged by size: 

                2118    2007    1260     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2663      10 
   Size:         54    1425     343     831    2916

  Fragments arranged by size: 

                2916    1425     831     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2676    3527    3886    4045     518 
   Size:        648    1510     851     359     159    2042

  Fragments arranged by size: 

                2042    1510     851     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2063    2087    2689    2838    2985    3662    4810    5055 
   Size:        457      24     602     149     147     677    1148     245

Cuts at:   5055    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     602     457     245     149     147
                  24
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5017
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5569
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5569
 
NotI GC'GGCC_GC

Cuts at:   3016    3016 
   Size:       5569
 
NsiI A_TGCA'T

Cuts at:   2553    2553 
   Size:       5569
 
NspI r_CATG'y

Cuts at:   1132    1229    2927    4472    1132 
   Size:         97    1698    1545    2229

  Fragments arranged by size: 

                2229    1698    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5569
 
PciI A'CATG_T

Cuts at:   4468    4468 
   Size:       5569
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5569
 
PmeI GTTT'AAAC

Cuts at:   2873    2873 
   Size:       5569
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3638    3670    5151    5183    3638 
   Size:         32    1481      32    4024

  Fragments arranged by size: 

                4024    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2296    2296 
   Size:       5569
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5569
 
PsiI TTA'TAA

Cuts at:   3139    3139 
   Size:       5569
 
PstI C_TGCA'G

Cuts at:    830    2757    2921     830 
   Size:       1927     164    3478

  Fragments arranged by size: 

                3478    1927     164
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5569
 
PvuII CAG'CTG

Cuts at:   2063    2689    2063 
   Size:        626    4943
 
SacI G_AGCT'C

Cuts at:   2364    2364 
   Size:       5569
 
SacII CC_GC'GG

Cuts at:   2839    2839 
   Size:       5569
 
SalI G'TCGA_C

Cuts at:   1936    2911    1936 
   Size:        975    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2064    2064 
   Size:       5569
 
SbfI CC_TGCA'GG

Cuts at:   2757    2921    2757 
   Size:        164    5405
 
ScaI AGT'ACT

Cuts at:   3817    5529    3817 
   Size:       1712    3857
 
SfcI C'TryA_G

Cuts at:    826    1045    2753    2917    4052    4733    4924     826 
   Size:        219    1708     164    1135     681     191    1471

  Fragments arranged by size: 

                1708    1471    1135     681     219     191     164
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5569
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5569
 
SmaI CCC'GGG

Cuts at:   1872    2896    1872 
   Size:       1024    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3673    4574    4836     820 
   Size:        197     926    1730     901     262    1553

  Fragments arranged by size: 

                1730    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3360     493 
   Size:       2867    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5569
 
SphI G_CATG'C

Cuts at:   1132    2927    1132 
   Size:       1795    3774
 
SspI AAT'ATT

Cuts at:      5      52    3493       5 
   Size:         47    3441    2081

  Fragments arranged by size: 

                3441    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5569
 
StyI C'CwwG_G

Cuts at:    513    1065    2703    3008     513 
   Size:        552    1638     305    3074

  Fragments arranged by size: 

                3074    1638     552     305
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2132    3596    3613    3766    3951    4396     325 
   Size:       1255     552    1464      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1464    1255     552     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3815    5527      96 
   Size:        274      80      33      51    1443    1838    1712     138

  Fragments arranged by size: 

                1838    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2241    2273    5529    5561    2241 
   Size:         32    3256      32    2249

  Fragments arranged by size: 

                3256    2249      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5569
 
XbaI T'CTAG_A

Cuts at:   2905    2905 
   Size:       5569
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5569
 
XmaI C'CCGG_G

Cuts at:   1870    2894    1870 
   Size:       1024    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3698    3698 
   Size:       5569
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2393    3377     276 
   Size:         53      83     186     936     859     984    2468

  Fragments arranged by size: 

                2468     984     936     859     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
     BplI    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI    RsrII    SanDI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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