(Linear) (Six-base) MAPSORT of: KIEE5316.seq Check: 9516 from: 1 to: 855 KIEE5316.orf With 182 enzymes: SgfI * January 29, 2014 11:55 .. AatII G_ACGT'C Cuts at: 0 390 855 Size: 390 465 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 185 479 855 Size: 116 69 294 376 Fragments arranged by size: 376 294 116 69 AlwNI CAG_nnn'CTG Cuts at: 0 106 312 855 Size: 106 206 543 Fragments arranged by size: 543 206 106 BanI G'GyrC_C Cuts at: 0 222 855 Size: 222 633 BanII G_rGCy'C Cuts at: 0 359 471 855 Size: 359 112 384 Fragments arranged by size: 384 359 112 BbsI GAAGACnn'nnnn_ Cuts at: 0 574 855 Size: 574 281 BbvCI CC'TCA_GC Cuts at: 0 503 676 855 Size: 503 173 179 Fragments arranged by size: 503 179 173 BfrBI ATG'CAT Cuts at: 0 546 855 Size: 546 309 Bpu10I CC'TnA_GC Cuts at: 0 503 676 855 Size: 503 173 179 Fragments arranged by size: 503 179 173 BsaI GGTCTCn'nnnn_ Cuts at: 0 103 112 855 Size: 103 9 743 Fragments arranged by size: 743 103 9 BsaHI Gr'CG_yC Cuts at: 0 387 855 Size: 387 468 BsaWI w'CCGG_w Cuts at: 0 272 419 855 Size: 272 147 436 Fragments arranged by size: 436 272 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 236 266 855 Size: 236 30 589 Fragments arranged by size: 589 236 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 52 55 88 130 196 361 364 Size: 49 3 3 33 42 66 165 3 Cuts at: 364 367 493 496 499 648 855 Size: 3 126 3 3 149 207 Fragments arranged by size: 207 165 149 126 66 49 42 33 3 3 3 3 3 3 BsiHKAI G_wGCw'C Cuts at: 0 359 855 Size: 359 496 BsmBI CGTCTCn'nnnn_ Cuts at: 0 378 855 Size: 378 477 Bsp1286I G_dGCh'C Cuts at: 0 359 471 855 Size: 359 112 384 Fragments arranged by size: 384 359 112 BspEI T'CCGG_A Cuts at: 0 419 855 Size: 419 436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 746 855 Size: 746 109 BsrBI CCG'CTC Cuts at: 0 399 429 441 576 603 855 Size: 399 30 12 135 27 252 Fragments arranged by size: 399 252 135 30 27 12 BstYI r'GATC_y Cuts at: 0 233 855 Size: 233 622 BtgI C'CryG_G Cuts at: 0 822 855 Size: 822 33 EaeI y'GGCC_r Cuts at: 0 647 855 Size: 647 208 EarI CTCTTCn'nnn_ Cuts at: 0 8 20 59 80 140 455 583 855 Size: 8 12 39 21 60 315 128 272 Fragments arranged by size: 315 272 128 60 39 21 12 8 EcoICRI GAG'CTC Cuts at: 0 357 855 Size: 357 498 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 185 479 855 Size: 116 69 294 376 Fragments arranged by size: 376 294 116 69 EcoO109I rG'GnC_Cy Cuts at: 0 291 855 Size: 291 564 HaeII r_GCGC'y Cuts at: 0 313 849 855 Size: 313 536 6 Fragments arranged by size: 536 313 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 306 338 855 Size: 306 32 517 Fragments arranged by size: 517 306 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 560 855 Size: 560 295 MscI TGG'CCA Cuts at: 0 649 855 Size: 649 206 MslI CAynn'nnrTG Cuts at: 0 662 855 Size: 662 193 MspA1I CmG'CkG Cuts at: 0 58 82 675 824 855 Size: 58 24 593 149 31 Fragments arranged by size: 593 149 58 31 24 NsiI A_TGCA'T Cuts at: 0 548 855 Size: 548 307 PpuMI rG'GwC_Cy Cuts at: 0 291 855 Size: 291 564 PstI C_TGCA'G Cuts at: 0 743 855 Size: 743 112 PvuII CAG'CTG Cuts at: 0 58 675 855 Size: 58 617 180 Fragments arranged by size: 617 180 58 SacI G_AGCT'C Cuts at: 0 359 855 Size: 359 496 SacII CC_GC'GG Cuts at: 0 825 855 Size: 825 30 SapI GCTCTTCn'nnn_ Cuts at: 0 59 855 Size: 59 796 SbfI CC_TGCA'GG Cuts at: 0 743 855 Size: 743 112 SfcI C'TryA_G Cuts at: 0 739 855 Size: 739 116 StyI C'CwwG_G Cuts at: 0 689 855 Size: 689 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 127 855 Size: 127 728 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 236 268 855 Size: 236 32 587 Fragments arranged by size: 587 236 32 ZraI GAC'GTC Cuts at: 0 388 855 Size: 388 467 Enzymes that do cut: AatII AcuI AlwNI BanI BanII BbsI BbvCI BfrBI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NsiI PpuMI PstI PvuII SacI SacII SapI SbfI SfcI StyI TaqII TstI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI BseYI BsgI BsiEI BsiWI BsmI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI