(Linear) (Six-base) MAPSORT of: KIBB5728.seq Check: 265 from: 1 to: 2193 KIBB5728.orf With 182 enzymes: SgfI * March 22, 2012 15:31 .. AccI GT'mk_AC Cuts at: 0 1624 2193 Size: 1624 569 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1574 2193 Size: 1574 619 AfeI AGC'GCT Cuts at: 0 137 2193 Size: 137 2056 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 595 627 2193 Size: 595 32 1566 Fragments arranged by size: 1566 595 32 AlwNI CAG_nnn'CTG Cuts at: 0 1597 2193 Size: 1597 596 ApaI G_GGCC'C Cuts at: 0 190 2193 Size: 190 2003 ApoI r'AATT_y Cuts at: 0 796 1171 1282 1684 2092 2193 Size: 796 375 111 402 408 101 Fragments arranged by size: 796 408 402 375 111 101 AvaI C'yCGr_G Cuts at: 0 46 171 181 2193 Size: 46 125 10 2012 Fragments arranged by size: 2012 125 46 10 AvrII C'CTAG_G Cuts at: 0 315 2193 Size: 315 1878 BamHI G'GATC_C Cuts at: 0 1572 2193 Size: 1572 621 BanI G'GyrC_C Cuts at: 0 210 2193 Size: 210 1983 BanII G_rGCy'C Cuts at: 0 31 190 368 2193 Size: 31 159 178 1825 Fragments arranged by size: 1825 178 159 31 BbeI G_GCGC'C Cuts at: 0 214 2193 Size: 214 1979 BbsI GAAGACnn'nnnn_ Cuts at: 0 1100 2193 Size: 1100 1093 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 381 415 2193 Size: 381 34 1778 Fragments arranged by size: 1778 381 34 BciVI GTATCCnnnnn_n' Cuts at: 0 560 2193 Size: 560 1633 BglI GCCn_nnn'nGGC Cuts at: 0 185 2193 Size: 185 2008 Bme1580I G_kGCm'C Cuts at: 0 190 2121 2193 Size: 190 1931 72 Fragments arranged by size: 1931 190 72 BmtI G_CTAG'C Cuts at: 0 1463 2193 Size: 1463 730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 986 1337 2133 2193 Size: 294 692 351 796 60 Fragments arranged by size: 796 692 351 294 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 787 2193 Size: 787 1406 BsaHI Gr'CG_yC Cuts at: 0 211 2193 Size: 211 1982 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 54 72 334 2193 Size: 54 18 262 1859 Fragments arranged by size: 1859 262 54 18 BseYI C'CCAG_C Cuts at: 0 34 283 2193 Size: 34 249 1910 Fragments arranged by size: 1910 249 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 1745 2193 Size: 757 988 448 Fragments arranged by size: 988 757 448 BsmI GAATG_Cn' Cuts at: 0 1186 2193 Size: 1186 1007 Bsp1286I G_dGCh'C Cuts at: 0 31 190 368 2121 2193 Size: 31 159 178 1753 72 Fragments arranged by size: 1753 178 159 72 31 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1411 2193 Size: 1411 782 BsrFI r'CCGG_y Cuts at: 0 166 289 2193 Size: 166 123 1904 Fragments arranged by size: 1904 166 123 BstBI TT'CG_AA Cuts at: 0 1411 2193 Size: 1411 782 BstYI r'GATC_y Cuts at: 0 842 1572 2193 Size: 842 730 621 Fragments arranged by size: 842 730 621 BtgI C'CryG_G Cuts at: 0 116 248 2193 Size: 116 132 1945 Fragments arranged by size: 1945 132 116 BtsI GCAGTG_nn' Cuts at: 0 1111 1376 1470 2193 Size: 1111 265 94 723 Fragments arranged by size: 1111 723 265 94 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 445 480 2193 Size: 445 35 1713 Fragments arranged by size: 1713 445 35 DraIII CAC_nnn'GTG Cuts at: 0 630 2193 Size: 630 1563 EaeI y'GGCC_r Cuts at: 0 275 453 645 704 2193 Size: 275 178 192 59 1489 Fragments arranged by size: 1489 275 192 178 59 EarI CTCTTCn'nnn_ Cuts at: 0 102 863 1534 1701 2193 Size: 102 761 671 167 492 Fragments arranged by size: 761 671 492 167 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 66 2193 Size: 66 2127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 986 1337 1574 2133 2193 Size: 294 692 351 237 559 60 Fragments arranged by size: 692 559 351 294 237 60 EcoO109I rG'GnC_Cy Cuts at: 0 50 186 2193 Size: 50 136 2007 Fragments arranged by size: 2007 136 50 EcoRI G'AATT_C Cuts at: 0 796 2193 Size: 796 1397 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 712 2193 Size: 712 1481 HaeII r_GCGC'y Cuts at: 0 139 214 290 2193 Size: 139 75 76 1903 Fragments arranged by size: 1903 139 76 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 329 361 2119 2151 2193 Size: 329 32 1758 32 42 Fragments arranged by size: 1758 329 42 32 32 HincII GTy'rAC Cuts at: 0 1557 2193 Size: 1557 636 KasI G'GCGC_C Cuts at: 0 210 2193 Size: 210 1983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 704 1145 1411 1528 2193 Size: 704 441 266 117 665 Fragments arranged by size: 704 665 441 266 117 MscI TGG'CCA Cuts at: 0 455 647 706 2193 Size: 455 192 59 1487 Fragments arranged by size: 1487 455 192 59 MslI CAynn'nnrTG Cuts at: 0 1745 1919 2193 Size: 1745 174 274 Fragments arranged by size: 1745 274 174 MspA1I CmG'CkG Cuts at: 0 34 269 323 1421 2193 Size: 34 235 54 1098 772 Fragments arranged by size: 1098 772 235 54 34 NaeI GCC'GGC Cuts at: 0 168 291 2193 Size: 168 123 1902 Fragments arranged by size: 1902 168 123 NarI GG'CG_CC Cuts at: 0 211 2193 Size: 211 1982 NdeI CA'TA_TG Cuts at: 0 1236 2193 Size: 1236 957 NgoMIV G'CCGG_C Cuts at: 0 166 289 2193 Size: 166 123 1904 Fragments arranged by size: 1904 166 123 NheI G'CTAG_C Cuts at: 0 1459 2193 Size: 1459 734 NspI r_CATG'y Cuts at: 0 772 2193 Size: 772 1421 PfoI T'CCnGG_A Cuts at: 0 1361 2193 Size: 1361 832 PpuMI rG'GwC_Cy Cuts at: 0 50 2193 Size: 50 2143 PshAI GACnn'nnGTC Cuts at: 0 1512 2193 Size: 1512 681 PspOMI G'GGCC_C Cuts at: 0 186 2193 Size: 186 2007 PvuII CAG'CTG Cuts at: 0 269 1421 2193 Size: 269 1152 772 Fragments arranged by size: 1152 772 269 SfcI C'TryA_G Cuts at: 0 781 2193 Size: 781 1412 SfiI GGCCn_nnn'nGGCC Cuts at: 0 185 2193 Size: 185 2008 SfoI GGC'GCC Cuts at: 0 212 2193 Size: 212 1981 SgrAI Cr'CCGG_yG Cuts at: 0 289 2193 Size: 289 1904 SmaI CCC'GGG Cuts at: 0 48 2193 Size: 48 2145 SmlI C'TyrA_G Cuts at: 0 802 2193 Size: 802 1391 SpeI A'CTAG_T Cuts at: 0 732 2193 Size: 732 1461 SspI AAT'ATT Cuts at: 0 537 2193 Size: 537 1656 StuI AGG'CCT Cuts at: 0 1940 2193 Size: 1940 253 StyI C'CwwG_G Cuts at: 0 315 1039 2193 Size: 315 724 1154 Fragments arranged by size: 1154 724 315 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2084 2193 Size: 2084 109 TatI w'GTAC_w Cuts at: 0 1997 2193 Size: 1997 196 Tth111I GACn'n_nGTC Cuts at: 0 1354 2193 Size: 1354 839 XbaI T'CTAG_A Cuts at: 0 1632 2193 Size: 1632 561 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1707 2193 Size: 1707 486 XmaI C'CCGG_G Cuts at: 0 46 2193 Size: 46 2147 XmnI GAAnn'nnTTC Cuts at: 0 575 2193 Size: 575 1618 Enzymes that do cut: AccI AcuI AfeI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BglI Bme1580I BmtI BpmI BpuEI BsaHI BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrFI BstBI BstYI BtgI BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NspI PfoI PpuMI PshAI PspOMI PvuII SfcI SfiI SfoI SgrAI SmaI SmlI SpeI SspI StuI StyI TaqII TatI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI BaeI BbvCI BclI BfrBI BglII BlpI BmgBI BmrI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SgfI SnaBI SphI SrfI SwaI TstI XhoI ZraI