(Linear) (Six-base) MAPSORT of: KIEE5490.seq Check: 7011 from: 1 to: 1029 KIEE5490.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 794 1029 Size: 794 235 AleI CACnn'nnGTG Cuts at: 0 149 631 1029 Size: 149 482 398 Fragments arranged by size: 482 398 149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 262 1029 Size: 262 767 BamHI G'GATC_C Cuts at: 0 523 1029 Size: 523 506 BciVI GTATCCnnnnn_n' Cuts at: 0 517 1029 Size: 517 512 BglII A'GATC_T Cuts at: 0 293 1029 Size: 293 736 BlpI GC'TnA_GC Cuts at: 0 637 1029 Size: 637 392 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 172 1029 Size: 172 857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 1029 Size: 310 719 BsaWI w'CCGG_w Cuts at: 0 737 1029 Size: 737 292 BseYI C'CCAG_C Cuts at: 0 1021 1029 Size: 1021 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 633 1029 Size: 232 401 396 Fragments arranged by size: 401 396 232 BsmI GAATG_Cn' Cuts at: 0 767 1029 Size: 767 262 BspEI T'CCGG_A Cuts at: 0 737 1029 Size: 737 292 BspHI T'CATG_A Cuts at: 0 488 1029 Size: 488 541 BsrDI GCAATG_nn' Cuts at: 0 440 719 1029 Size: 440 279 310 Fragments arranged by size: 440 310 279 BstXI CCAn_nnnn'nTGG Cuts at: 0 403 1029 Size: 403 626 BstYI r'GATC_y Cuts at: 0 293 523 602 1029 Size: 293 230 79 427 Fragments arranged by size: 427 293 230 79 BtgI C'CryG_G Cuts at: 0 362 1029 Size: 362 667 BtsI GCAGTG_nn' Cuts at: 0 846 1029 Size: 846 183 EaeI y'GGCC_r Cuts at: 0 86 437 1029 Size: 86 351 592 Fragments arranged by size: 592 351 86 EarI CTCTTCn'nnn_ Cuts at: 0 127 346 1000 1029 Size: 127 219 654 29 Fragments arranged by size: 654 219 127 29 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 794 1029 Size: 310 484 235 Fragments arranged by size: 484 310 235 FspI TGC'GCA Cuts at: 0 143 1029 Size: 143 886 FspAI rTGC'GCAy Cuts at: 0 143 1029 Size: 143 886 HincII GTy'rAC Cuts at: 0 83 1029 Size: 83 946 MfeI C'AATT_G Cuts at: 0 899 1029 Size: 899 130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 501 620 1029 Size: 501 119 409 Fragments arranged by size: 501 409 119 MscI TGG'CCA Cuts at: 0 439 1029 Size: 439 590 MslI CAynn'nnrTG Cuts at: 0 149 232 361 631 1029 Size: 149 83 129 270 398 Fragments arranged by size: 398 270 149 129 83 MspA1I CmG'CkG Cuts at: 0 513 694 772 1029 Size: 513 181 78 257 Fragments arranged by size: 513 257 181 78 NspI r_CATG'y Cuts at: 0 142 1029 Size: 142 887 PfoI T'CCnGG_A Cuts at: 0 287 1029 Size: 287 742 PstI C_TGCA'G Cuts at: 0 383 1029 Size: 383 646 PvuII CAG'CTG Cuts at: 0 513 772 1029 Size: 513 259 257 Fragments arranged by size: 513 259 257 SapI GCTCTTCn'nnn_ Cuts at: 0 346 1029 Size: 346 683 SfcI C'TryA_G Cuts at: 0 379 948 1029 Size: 379 569 81 Fragments arranged by size: 569 379 81 SphI G_CATG'C Cuts at: 0 142 1029 Size: 142 887 SspI AAT'ATT Cuts at: 0 10 1029 Size: 10 1019 StuI AGG'CCT Cuts at: 0 790 1029 Size: 790 239 TatI w'GTAC_w Cuts at: 0 386 1029 Size: 386 643 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 517 1029 Size: 517 512 Enzymes that do cut: AcuI AleI AlfI BamHI BciVI BglII BlpI BplI BpmI BsaWI BseYI BsgI BsmI BspEI BspHI BsrDI BstXI BstYI BtgI BtsI EaeI EarI Eco57MI FspI FspAI HincII MfeI MmeI MscI MslI MspA1I NspI PfoI PstI PvuII SapI SfcI SphI SspI StuI TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I HindIII HpaI KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StyI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI