################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvaI AviII BamHI BbrPI BciVI BclI BfrI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HgaI HindIII KpnI KspI MamI MluI MroI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 1251 GACGTC GACGT^C AhdI 1822 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 135 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1822 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1084 CCTAGG C^CTAGG BbeI 1076 GGCGCC GGCGC^C BbvCI 1293 CCTCAGC CCTCAGC(-5/-2) BglII 287 AGATCT A^GATCT BlnI 1084 CCTAGG C^CTAGG BlpI 353 GCTTAGC GC^T[ACGT]AGC Bpu1102I 353 GCTTAGC GC^T[ACGT]AGC BsgI 1153 GTGCAG GTGCAG(16/14) BsrDI 1113 CATTGC GCAATG(2/0) Bst1107I 1882 GTATAC GTA^TAC BstZ17I 1882 GTATAC GTA^TAC CelII 353 GCTTAGC GC^T[ACGT]AGC Eam1105I 1822 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 400 ATGCAT ATGCA^T HpaI 793 GTTAAC GTT^AAC KasI 1076 GGCGCC G^GCGCC MfeI 896 CAATTG C^AATTG MspA1I 1273 CAGCTG C[AC]G^C[GT]G MunI 896 CAATTG C^AATTG NarI 1076 GGCGCC GG^CGCC NdeI 1888 CATATG CA^TATG NsiI 400 ATGCAT ATGCA^T NspI 14 GCATGC [GA]CATG^[CT] PstI 881 CTGCAG CTGCA^G PvuII 1273 CAGCTG CAG^CTG SalI 1819 GTCGAC G^TCGAC SexAI 593 ACCTGGT A^CC[AT]GGT SfoI 1076 GGCGCC GGC^GCC SmlI 1509 CTTGAG C^T[CT][GA]AG SphI 14 GCATGC GCATG^C StuI 584 AGGCCT AGG^CCT XcmI 1533 CCATGGCCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 135 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 60 CGCG CG^CG 481 CGCG AcyI 1076 GGCGCC G[GA]^CG[CT]C 1251 GACGTC AfaI 516 GTAC GT^AC 1667 GTAC AlwI 33 GATCC GGATC(4/5) 555 GGATC AspI 1180 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1804 GACTACGTC BanI 238 GGTGCC G^G[CT][GA]CC 1076 GGCGCC BanII 1512 GAGCTC G[GA]GC[CT]^C 1690 GAGCTC BbsI 625 GAAGAC GAAGAC(2/6) 1177 GAAGAC BcgI 1623 CGACTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1448 GCAACCGTGTCG BfaI 722 CTAG C^TAG 1085 CTAG BpmI 1030 CTGGAG CTGGAG(16/14) 1337 CTGGAG BpuAI 625 GAAGAC GAAGAC(2/6) 1177 GAAGAC BsaHI 1076 GGCGCC G[GA]^CG[CT]C 1251 GACGTC BseRI 108 CTCCTC GAGGAG(10/8) 676 GAGGAG BssSI 273 CACGAG CACGAG(-5/-1) 997 CTCGTG BstUI 60 CGCG CG^CG 481 CGCG BstXI 1464 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1527 CCATGGCCATGG BstYI 287 AGATCT [GA]^GATC[CT] 555 GGATCT EcoRV 852 GATATC GAT^ATC 985 GATATC FauI 1118 CCCGC CCCGC(4/6) 945 GCGGG HaeII 1076 GGCGCC [GA]GCGC^[CT] 1523 AGCGCC Hin1I 1076 GGCGCC G[GA]^CG[CT]C 1251 GACGTC HincII 793 GTTAAC GT[CT]^[GA]AC 1819 GTCGAC HindII 793 GTTAAC GT[CT]^[GA]AC 1819 GTCGAC MaeI 722 CTAG C^TAG 1085 CTAG MflI 287 AGATCT [GA]^GATC[CT] 555 GGATCT MvnI 60 CGCG CG^CG 481 CGCG PflFI 1180 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1804 GACTACGTC PflMI 1131 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1750 CCAAGCCCTGG RsaI 516 GTAC GT^AC 1667 GTAC SacI 1512 GAGCTC GAGCT^C 1690 GAGCTC SfiI 1269 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1323 GGCCCCCCTGGCC Tth111I 1180 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1804 GACTACGTC Van91I 1131 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1750 CCAAGCCCTGG XhoII 287 AGATCT [GA]^GATC[CT] 555 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccI AciI AcsI AluI AlwNI ApoI AspHI AvaII BalI BbvI BcnI BglI BmyI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I