################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrFI BssHII Bst1107I BstBI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HindIII HpaI KasI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 1290 GGTACC G^GTACC AflIII 47 ACACGT A^C[GA][CT]GT AlwI 381 GGATC GGATC(4/5) AlwNI 181 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1237 GGGCCC GGGCC^C Asp718I 1290 GGTACC G^GTACC AspHI 486 GAGCAC G[AT]GC[AT]^C AvaII 875 GGACC G^G[AT]CC BanII 1237 GGGCCC G[GA]GC[CT]^C BbrPI 48 CACGTG CAC^GTG BbsI 1069 GAAGAC GAAGAC(2/6) BclI 1304 TGATCA T^GATCA BfaI 160 CTAG C^TAG BpuAI 1069 GAAGAC GAAGAC(2/6) BsaAI 48 CACGTG [CT]AC^GT[GA] BsaBI 1200 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 486 GAGCAC G[AT]GC[AT]^C BsmAI 690 GAGAC GTCTC(1/5) Bsp1407I 677 TGTACA T^GTACA BspHI 620 TCATGA T^CATGA BsrBI 99 CCGCTC CCGCTC(-3/-3) BsrDI 533 GCAATG GCAATG(2/0) BsrGI 677 TGTACA T^GTACA BssSI 247 CTCGTG CACGAG(-5/-1) DdeI 583 CTCAG C^T[ACGT]AG FbaI 1304 TGATCA T^GATCA HgaI 353 GCGTC GACGC(5/10) KpnI 1290 GGTACC GGTAC^C KspI 438 CCGCGG CCGC^GG MaeI 160 CTAG C^TAG MamI 1200 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflMI 548 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 710 GAGTC GAGTC(4/5) PmaCI 48 CACGTG CAC^GTG PmlI 48 CACGTG CAC^GTG PshAI 1003 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 181 CAGCTG CAG^CTG RcaI 620 TCATGA T^CATGA SacII 438 CCGCGG CCGC^GG SspBI 677 TGTACA T^GTACA TfiI 1507 GATTC G^A[AT]TC Tsp509I 1081 AATT ^AATT Van91I 548 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 496 CCACTCTACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 3 GACGTC GACGT^C 1096 GACGTC AcyI 3 GACGTC G[GA]^CG[CT]C 1096 GACGTC AfaI 678 GTAC GT^AC 1291 GTAC Asp700I 1407 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1410 GAAGTTCTTC BanI 460 GGCACC G^G[CT][GA]CC 1290 GGTACC BmyI 486 GAGCAC G[AGT]GC[ACT]^C 1237 GGGCCC BsaHI 3 GACGTC G[GA]^CG[CT]C 1096 GACGTC Bsp1286I 486 GAGCAC G[AGT]GC[ACT]^C 1237 GGGCCC BstAPI 180 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 666 GCATGCTCTGC BstEII 192 GGTGACC G^GT[ACGT]ACC 442 GGTTACC BstPI 192 GGTGACC G^GT[ACGT]ACC 442 GGTTACC BstXI 74 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 362 CCATTGTCGTGG DraII 1236 GGGGCCC [GA]G^G[ACGT]CC[CT] 1237 GGGCCCT EarI 283 CTCTTC CTCTTC(1/4) 739 GAAGAG EcoO109I 1236 GGGGCCC [GA]G^G[ACGT]CC[CT] 1237 GGGCCCT EcoO65I 192 GGTGACC G^GT[ACGT]ACC 442 GGTTACC HaeII 156 GGCGCT [GA]GCGC^[CT] 434 AGCGCC Hin1I 3 GACGTC G[GA]^CG[CT]C 1096 GACGTC HincII 355 GTCAAC GT[CT]^[GA]AC 1021 GTCAAC HindII 355 GTCAAC GT[CT]^[GA]AC 1021 GTCAAC HinfI 710 GAGTC G^A[ACGT]TC 1507 GATTC Ksp632I 283 CTCTTC CTCTTC(1/4) 739 GAAGAG MseI 212 TTAA T^TAA 1382 TTAA MspA1I 181 CAGCTG C[AC]G^C[GT]G 438 CCGCGG RsaI 678 GTAC GT^AC 1291 GTAC SfaNI 92 GCATC GCATC(5/9) 1343 GCATC SphI 53 GCATGC GCATG^C 666 GCATGC TaqI 1100 TCGA T^CGA 1280 TCGA Tru9I 212 TTAA T^TAA 1382 TTAA Tsp45I 193 GTGAC ^GT[GC]AC 223 GTCAC TthHB8I 1100 TCGA T^CGA 1280 TCGA XmnI 1407 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1410 GAAGTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AluI BalI BbvI BcnI BsaJI BseRI BsgI BsiYI BslI BsmFI BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PstI Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI