(Circular) (Six-base) MAPSORT of: pFN21AE9401.seq Check: 5398 from: 1 to: 6229 pFN21AE9401 6229 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2024 2185 2024 Size: 161 6068 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2012 3105 4039 278 Size: 53 83 186 936 476 1093 934 2468 Fragments arranged by size: 2468 1093 936 934 476 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3295 3550 3295 Size: 255 5974 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2876 3431 3507 4292 5676 5944 1632 Size: 1244 555 76 785 1384 268 1917 Fragments arranged by size: 1917 1384 1244 785 555 268 76 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 2052 5128 6105 1203 Size: 849 3076 977 1327 Fragments arranged by size: 3076 1327 977 849 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 2279 1166 Size: 1113 5116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2171 1184 Size: 987 5242 AlwNI CAG_nnn'CTG Cuts at: 2191 3972 5544 2191 Size: 1781 1572 2876 Fragments arranged by size: 2876 1781 1572 ApaI G_GGCC'C Cuts at: 3246 3246 Size: 6229 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 3538 3764 3916 6025 1493 Size: 2045 226 152 2109 1697 Fragments arranged by size: 2109 2045 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 3554 1870 Size: 73 1611 4545 Fragments arranged by size: 4545 1611 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3279 3287 3312 3320 3279 Size: 8 25 8 6188 Fragments arranged by size: 6188 25 8 8 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 3295 3550 5005 618 Size: 325 217 627 678 830 255 1455 1842 Fragments arranged by size: 1842 1455 830 678 627 325 255 217 BanII G_rGCy'C Cuts at: 1391 3246 3548 1391 Size: 1855 302 4072 Fragments arranged by size: 4072 1855 302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3081 928 Size: 971 1182 4076 Fragments arranged by size: 4076 1182 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4420 4454 1903 Size: 34 2483 34 3678 Fragments arranged by size: 3678 2483 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 3309 1548 Size: 1761 4468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4840 136 Size: 107 122 71 631 605 3168 1525 Fragments arranged by size: 3168 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6224 6224 Size: 6229 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 3246 4290 5446 1790 Size: 1456 1044 1156 2573 Fragments arranged by size: 2573 1456 1156 1044 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 3295 3960 Size: 416 53 83 399 24 1715 163 665 Cuts at: 3960 4918 442 Size: 958 1753 Fragments arranged by size: 1753 1715 958 665 416 399 163 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2514 2514 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4889 1239 Size: 3650 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4354 5219 5517 4354 Size: 865 298 5066 Fragments arranged by size: 5066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4892 882 Size: 617 3393 2219 Fragments arranged by size: 3393 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2055 4020 493 Size: 711 851 1965 2702 Fragments arranged by size: 2702 1965 851 711 BsaBI GATnn'nnATC Cuts at: 3209 6223 3209 Size: 3014 3215 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3102 4036 4418 Size: 53 83 186 936 476 1093 934 382 Cuts at: 4418 5053 275 Size: 635 1451 Fragments arranged by size: 1451 1093 936 934 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2399 2429 1178 Size: 30 165 30 996 30 4978 Fragments arranged by size: 4978 996 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2447 2487 2780 2930 3002 3156 1186 Size: 1027 234 40 293 150 72 154 4259 Fragments arranged by size: 4259 1027 293 234 154 150 72 40 BseYI C'CCAG_C Cuts at: 2436 2511 2573 5432 5928 2436 Size: 75 62 2859 496 2737 Fragments arranged by size: 2859 2737 496 75 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2002 2299 2676 3055 1163 Size: 573 266 297 377 379 4337 Fragments arranged by size: 4337 573 379 377 297 266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 4440 5468 1540 Size: 275 186 1678 761 1028 2301 Fragments arranged by size: 2301 1678 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2495 4290 4375 5446 2495 Size: 1795 85 1071 3278 Fragments arranged by size: 3278 1795 1071 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2495 3246 3548 4290 4375 5446 1391 Size: 399 705 751 302 742 85 1071 2174 Fragments arranged by size: 2174 1071 751 742 705 399 302 85 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 2625 4118 5848 2625 Size: 1493 1730 3006 Fragments arranged by size: 3006 1730 1493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2024 2185 2244 2460 3471 3584 844 Size: 1180 161 59 216 1011 113 3489 Fragments arranged by size: 3489 1180 1011 216 161 113 59 BsrBI CCG'CTC Cuts at: 1394 1823 2106 4116 1394 Size: 429 283 2010 3507 Fragments arranged by size: 3507 2010 429 283 BsrDI GCAATG_nn' Cuts at: 65 1248 2545 4724 4898 65 Size: 1183 1297 2179 174 1396 Fragments arranged by size: 2179 1396 1297 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4873 5060 1700 Size: 110 3063 187 2869 Fragments arranged by size: 3063 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2682 96 Size: 2586 3643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 2252 4289 5301 2252 Size: 2037 1012 3180 Fragments arranged by size: 3180 2037 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2191 2677 794 Size: 1397 486 4346 Fragments arranged by size: 4346 1397 486 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 2197 2447 1184 Size: 1013 250 4966 Fragments arranged by size: 4966 1013 250 BstXI CCAn_nnnn'nTGG Cuts at: 2086 2374 2086 Size: 288 5941 BstYI r'GATC_y Cuts at: 1972 3559 3926 4311 4328 5780 5866 5878 6224 Size: 1587 367 385 17 1452 86 12 346 Cuts at: 6224 1972 Size: 1977 Fragments arranged by size: 1977 1587 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2092 2443 2643 513 Size: 552 1027 351 200 4099 Fragments arranged by size: 4099 1027 552 351 200 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 3117 3347 3745 4539 4559 3117 Size: 230 398 794 20 4787 Fragments arranged by size: 4787 794 398 230 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2478 2940 Size: 54 1425 50 275 18 441 207 462 Cuts at: 2940 3267 3676 4565 6194 8 Size: 327 409 889 1629 43 Fragments arranged by size: 1629 1425 889 462 441 409 327 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2294 2739 4159 1353 Size: 941 445 1420 3423 Fragments arranged by size: 3423 1420 941 445 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2876 3431 3507 4292 4889 5676 5944 Size: 393 1244 555 76 785 597 787 268 Cuts at: 5944 1239 Size: 1524 Fragments arranged by size: 1524 1244 787 785 597 555 393 268 76 EcoO109I rG'GnC_Cy Cuts at: 3242 3243 3242 Size: 1 6228 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3436 3436 Size: 6229 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2165 2443 5376 1678 Size: 487 278 2933 2531 Fragments arranged by size: 2933 2531 487 278 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373 Size: 32 135 32 3298 32 42 32 2626 Fragments arranged by size: 3298 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 3028 3573 3819 669 Size: 988 281 424 666 545 246 3079 Fragments arranged by size: 3079 988 666 545 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KpnI G_GTAC'C Cuts at: 3299 3554 3299 Size: 255 5974 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5343 5527 1305 Size: 4038 184 2007 Fragments arranged by size: 4038 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 2480 2942 10 Size: 54 1425 343 441 207 462 3297 Fragments arranged by size: 3297 1425 462 441 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2279 2372 2805 3357 4187 4546 Size: 648 891 222 93 433 552 830 359 Cuts at: 4546 4705 518 Size: 159 2042 Fragments arranged by size: 2042 891 830 648 552 433 359 222 159 93 MspA1I CmG'CkG Cuts at: 1606 2188 2445 3645 4322 5470 5715 1606 Size: 582 257 1200 677 1148 245 2120 Fragments arranged by size: 2120 1200 1148 677 582 257 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5677 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 2062 2675 3376 3409 3587 5132 1132 Size: 97 833 613 701 33 178 1545 2229 Fragments arranged by size: 2229 1545 833 701 613 178 97 33 PasI CC'CwG_GG Cuts at: 1348 2262 3238 1348 Size: 914 976 4339 Fragments arranged by size: 4339 976 914 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 2559 1108 Size: 1451 4778 PfoI T'CCnGG_A Cuts at: 2772 3120 3399 2772 Size: 348 279 5602 Fragments arranged by size: 5602 348 279 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PmlI CAC'GTG Cuts at: 2055 2055 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3012 1613 Size: 1399 4830 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 3242 3242 Size: 6229 PstI C_TGCA'G Cuts at: 830 2196 2244 2490 3342 3581 830 Size: 1366 48 246 852 239 3478 Fragments arranged by size: 3478 1366 852 246 239 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2188 2188 Size: 6229 SacII CC_GC'GG Cuts at: 2446 2446 Size: 6229 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SfcI C'TryA_G Cuts at: 826 1045 2192 2240 2486 3338 3577 4712 5393 Size: 219 1147 48 246 852 239 1135 681 Cuts at: 5393 5584 826 Size: 191 1471 Fragments arranged by size: 1471 1147 1135 852 681 246 239 219 191 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4333 5234 5496 820 Size: 197 926 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 2062 2675 3587 1132 Size: 930 613 912 3774 Fragments arranged by size: 3774 930 912 613 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6229 StyI C'CwwG_G Cuts at: 513 1065 2820 2841 2862 3668 513 Size: 552 1755 21 21 806 3074 Fragments arranged by size: 3074 1755 806 552 21 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 2351 3038 3151 3265 3295 3471 Size: 1255 674 97 687 113 114 30 176 Cuts at: 3471 4256 4273 4426 4611 5056 325 Size: 785 17 153 185 445 1498 Fragments arranged by size: 1498 1255 785 687 674 445 185 176 153 114 113 97 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2682 4475 6187 Size: 274 80 33 51 1443 705 1793 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1793 1712 1443 705 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2399 2431 6189 6221 2399 Size: 32 3758 32 2407 Fragments arranged by size: 3758 2407 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2508 2508 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 3416 3419 4358 3416 Size: 3 939 5287 Fragments arranged by size: 5287 939 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2010 3103 4037 276 Size: 53 83 186 936 476 1093 934 2468 Fragments arranged by size: 2468 1093 936 934 476 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI