Restriction map for
pFN21AE6772
(Linear) (Six-base) MAPSORT of: KIEE6772.seq Check: 2101 from: 1 to: 1839
KIEE6772.orf
With 182 enzymes: SgfI *
July 24, 2018 16:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 509 1839
Size: 335 174 1330
Fragments arranged by size:
1330 335 174
AleI CACnn'nnGTG
Cuts at: 0 985 1839
Size: 985 854
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 350 382 1839
Size: 350 32 1457
Fragments arranged by size:
1457 350 32
AlwNI CAG_nnn'CTG
Cuts at: 0 45 648 1049 1576 1839
Size: 45 603 401 527 263
Fragments arranged by size:
603 527 401 263 45
ApaI G_GGCC'C
Cuts at: 0 1462 1839
Size: 1462 377
ApoI r'AATT_y
Cuts at: 0 293 1839
Size: 293 1546
AvaI C'yCGr_G
Cuts at: 0 81 455 968 1003 1839
Size: 81 374 513 35 836
Fragments arranged by size:
836 513 374 81 35
BamHI G'GATC_C
Cuts at: 0 459 1839
Size: 459 1380
BanI G'GyrC_C
Cuts at: 0 48 241 381 1839
Size: 48 193 140 1458
Fragments arranged by size:
1458 193 140 48
BanII G_rGCy'C
Cuts at: 0 456 1205 1462 1839
Size: 456 749 257 377
Fragments arranged by size:
749 456 377 257
BbeI G_GCGC'C
Cuts at: 0 385 1839
Size: 385 1454
BbvCI CC'TCA_GC
Cuts at: 0 1042 1839
Size: 1042 797
BciVI GTATCCnnnnn_n'
Cuts at: 0 1591 1839
Size: 1591 248
BclI T'GATC_A
Cuts at: 0 1365 1839
Size: 1365 474
BglI GCCn_nnn'nGGC
Cuts at: 0 1428 1839
Size: 1428 411
BglII A'GATC_T
Cuts at: 0 1797 1839
Size: 1797 42
BlpI GC'TnA_GC
Cuts at: 0 1418 1839
Size: 1418 421
Bme1580I G_kGCm'C
Cuts at: 0 53 982 1462 1839
Size: 53 929 480 377
Fragments arranged by size:
929 480 377 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 695 1839
Size: 695 1144
BmtI G_CTAG'C
Cuts at: 0 167 1839
Size: 167 1672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 839 1219 1415 1430 1467 1839
Size: 127 712 380 196 15 37 372
Fragments arranged by size:
712 380 372 196 127 37 15
Bpu10I CC'TnA_GC
Cuts at: 0 497 641 1042 1839
Size: 497 144 401 797
Fragments arranged by size:
797 497 401 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1839
Size: 356 1483
BsaHI Gr'CG_yC
Cuts at: 0 382 1839
Size: 382 1457
BsaWI w'CCGG_w
Cuts at: 0 5 1839
Size: 5 1834
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 350 380 812 842 1614 1644 1839
Size: 350 30 432 30 772 30 195
Fragments arranged by size:
772 432 350 195 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 509 1839
Size: 509 1330
BseYI C'CCAG_C
Cuts at: 0 1633 1839
Size: 1633 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 1839
Size: 1520 319
BsiHKAI G_wGCw'C
Cuts at: 0 954 1361 1839
Size: 954 407 478
Fragments arranged by size:
954 478 407
BsmI GAATG_Cn'
Cuts at: 0 1757 1839
Size: 1757 82
Bsp1286I G_dGCh'C
Cuts at: 0 53 456 954 982 1205 1361 1462 1839
Size: 53 403 498 28 223 156 101 377
Fragments arranged by size:
498 403 377 223 156 101 53 28
BspHI T'CATG_A
Cuts at: 0 1830 1839
Size: 1830 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 656 1839
Size: 656 1183
BsrFI r'CCGG_y
Cuts at: 0 95 1839
Size: 95 1744
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1312 1839
Size: 1312 527
BstYI r'GATC_y
Cuts at: 0 442 459 492 1025 1797 1839
Size: 442 17 33 533 772 42
Fragments arranged by size:
772 533 442 42 33 17
Bsu36I CC'TnA_GG
Cuts at: 0 1702 1839
Size: 1702 137
BtgI C'CryG_G
Cuts at: 0 1055 1839
Size: 1055 784
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 135 1839
Size: 135 1704
BtsI GCAGTG_nn'
Cuts at: 0 568 1131 1839
Size: 568 563 708
Fragments arranged by size:
708 568 563
ClaI AT'CG_AT
Cuts at: 0 191 1839
Size: 191 1648
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1209 1244 1839
Size: 1209 35 595
Fragments arranged by size:
1209 595 35
DraIII CAC_nnn'GTG
Cuts at: 0 1182 1839
Size: 1182 657
DrdI GACnn_nn'nnGTC
Cuts at: 0 556 1714 1839
Size: 556 1158 125
Fragments arranged by size:
1158 556 125
EaeI y'GGCC_r
Cuts at: 0 1217 1839
Size: 1217 622
EarI CTCTTCn'nnn_
Cuts at: 0 826 1003 1839
Size: 826 177 836
Fragments arranged by size:
836 826 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 335 509 839 1219 1415 1430 1467
Size: 127 208 174 330 380 196 15 37
Cuts at: 1467 1839
Size: 372
Fragments arranged by size:
380 372 330 208 196 174 127 37
15
EcoO109I rG'GnC_Cy
Cuts at: 0 645 1459 1839
Size: 645 814 380
Fragments arranged by size:
814 645 380
HaeII r_GCGC'y
Cuts at: 0 385 1839
Size: 385 1454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1588 1620 1839
Size: 1588 32 219
Fragments arranged by size:
1588 219 32
HindIII A'AGCT_T
Cuts at: 0 873 1839
Size: 873 966
KasI G'GCGC_C
Cuts at: 0 381 1839
Size: 381 1458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1095 1839
Size: 1095 744
MscI TGG'CCA
Cuts at: 0 1219 1839
Size: 1219 620
MslI CAynn'nnrTG
Cuts at: 0 985 1300 1839
Size: 985 315 539
Fragments arranged by size:
985 539 315
MspA1I CmG'CkG
Cuts at: 0 424 705 1189 1839
Size: 424 281 484 650
Fragments arranged by size:
650 484 424 281
NarI GG'CG_CC
Cuts at: 0 382 1839
Size: 382 1457
NcoI C'CATG_G
Cuts at: 0 1055 1839
Size: 1055 784
NheI G'CTAG_C
Cuts at: 0 163 1839
Size: 163 1676
NspI r_CATG'y
Cuts at: 0 130 513 1322 1839
Size: 130 383 809 517
Fragments arranged by size:
809 517 383 130
PflMI CCAn_nnn'nTGG
Cuts at: 0 1182 1839
Size: 1182 657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 350 382 1839
Size: 350 32 1457
Fragments arranged by size:
1457 350 32
PpuMI rG'GwC_Cy
Cuts at: 0 645 1839
Size: 645 1194
PspOMI G'GGCC_C
Cuts at: 0 1458 1839
Size: 1458 381
PspXI vC'TCGA_Gb
Cuts at: 0 968 1839
Size: 968 871
PstI C_TGCA'G
Cuts at: 0 438 1839
Size: 438 1401
PvuII CAG'CTG
Cuts at: 0 424 705 1189 1839
Size: 424 281 484 650
Fragments arranged by size:
650 484 424 281
RsrII CG'GwC_CG
Cuts at: 0 62 1839
Size: 62 1777
SfcI C'TryA_G
Cuts at: 0 434 1839
Size: 434 1405
SfoI GGC'GCC
Cuts at: 0 383 1839
Size: 383 1456
SmlI C'TyrA_G
Cuts at: 0 371 968 1839
Size: 371 597 871
Fragments arranged by size:
871 597 371
SphI G_CATG'C
Cuts at: 0 1322 1839
Size: 1322 517
StuI AGG'CCT
Cuts at: 0 17 1612 1839
Size: 17 1595 227
Fragments arranged by size:
1595 227 17
StyI C'CwwG_G
Cuts at: 0 53 930 974 1055 1839
Size: 53 877 44 81 784
Fragments arranged by size:
877 784 81 53 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1290 1839
Size: 1290 549
TatI w'GTAC_w
Cuts at: 0 699 1839
Size: 699 1140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 812 844 1839
Size: 812 32 995
Fragments arranged by size:
995 812 32
Tth111I GACn'n_nGTC
Cuts at: 0 1602 1839
Size: 1602 237
XbaI T'CTAG_A
Cuts at: 0 135 1839
Size: 135 1704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1212 1839
Size: 1212 627
XhoI C'TCGA_G
Cuts at: 0 968 1839
Size: 968 871
XmnI GAAnn'nnTTC
Cuts at: 0 989 1839
Size: 989 850
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrFI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EarI Eco57MI
EcoO109I HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NarI NcoI NheI NspI PflMI PpiI PpuMI
PspOMI PspXI PstI PvuII RsrII SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BbsI BcgI BfrBI BmgBI BplI BsaI BsaAI
BsaBI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I DraI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PsrI PvuI SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XmaI
ZraI