################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspHI BspLU11I BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 214 CGCG CG^CG AccIII 606 TCCGGA T^CCGGA AcsI 457 GAATTC [GA]^AATT[CT] AflIII 533 ACGTGT A^C[GA][CT]GT ApoI 457 GAATTC [GA]^AATT[CT] BalI 224 TGGCCA TGG^CCA BamHI 609 GGATCC G^GATCC BanI 361 GGTGCC G^G[CT][GA]CC BcnI 671 CCGGG CC^[GC]GG BfaI 421 CTAG C^TAG BglI 364 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 443 CTGGAG CTGGAG(16/14) BsaWI 606 TCCGGA [AT]^CCGG[AT] BseAI 606 TCCGGA T^CCGGA BsmI 599 GAATGC GAATGC(1/-1) Bsp1407I 749 TGTACA T^GTACA BspEI 606 TCCGGA T^CCGGA BsrBI 88 GAGCGG CCGCTC(-3/-3) BsrGI 749 TGTACA T^GTACA BsrI 321 CCAGT ACTGG(1/-1) BssSI 785 CTCGTG CACGAG(-5/-1) BstEII 217 GGTAACC G^GT[ACGT]ACC BstPI 217 GGTAACC G^GT[ACGT]ACC BstUI 214 CGCG CG^CG DdeI 835 CTCAG C^T[ACGT]AG DraII 512 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 166 GACTGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 512 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 217 GGTAACC G^GT[ACGT]ACC EcoRI 457 GAATTC G^AATTC FauI 90 GCGGG CCCGC(4/6) HincII 675 GTTGAC GT[CT]^[GA]AC HindII 675 GTTGAC GT[CT]^[GA]AC HindIII 803 AAGCTT A^AGCTT HinfI 345 GAATC G^A[ACGT]TC HphI 472 GGTGA GGTGA(8/7) MaeI 421 CTAG C^TAG MaeII 533 ACGT A^CGT MluNI 224 TGGCCA TGG^CCA MroI 606 TCCGGA T^CCGGA MscI 224 TGGCCA TGG^CCA MvnI 214 CGCG CG^CG NciI 671 CCGGG CC^[GC]GG NspI 117 GCATGC [GA]CATG^[CT] SphI 117 GCATGC GCATG^C SspBI 749 TGTACA T^GTACA StuI 465 AGGCCT AGG^CCT TaqI 86 TCGA T^CGA TfiI 345 GAATC G^A[AT]TC Tsp509I 458 AATT ^AATT TthHB8I 86 TCGA T^CGA XcmI 26 CCAAGGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 610 GATCC GGATC(4/5) 609 GGATC AvaII 174 GGTCC G^G[AT]CC 824 GGTCC BglII 290 AGATCT A^GATCT 488 AGATCT BsgI 112 GTGCAG GTGCAG(16/14) 541 GTGCAG BspMI 40 GCAGGT ACCTGC(4/8) 108 GCAGGT BstF5I 377 CATCC GGATG(2/0) 641 GGATG EaeI 50 TGGCCG [CT]^GGCC[GA] 224 TGGCCA FokI 377 CATCC GGATG(9/13) 641 GGATG HaeII 78 AGCGCC [GA]GCGC^[CT] 329 GGCGCT HapII 607 CCGG C^CGG 671 CCGG HpaII 607 CCGG C^CGG 671 CCGG MboII 777 GAAGA GAAGA(8/7) 293 TCTTC MslI 377 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 711 CATGATGATG MspI 607 CCGG C^CGG 671 CCGG PstI 106 CTGCAG CTGCA^G 137 CTGCAG PvuII 425 CAGCTG CAG^CTG 636 CAGCTG SfcI 106 CTGCAG C^T[GA][CT]AG 137 CTGCAG Tsp45I 473 GTGAC ^GT[GC]AC 658 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BsiYI BslI BssKI BstNI BstYI Cac8I CfoI Cfr13I DpnII EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I ItaI MaeIII MboI MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI TspRI XhoII