(Circular) (Six-base) MAPSORT of: pFN21AE4302.seq Check: 2822 from: 1 to: 5599 pFN21AE4302 5599 bp With 182 enzymes: SgfI * March 28, 2012 14:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2036 2104 2036 Size: 68 5531 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3409 278 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2942 1937 Size: 1005 4594 Acc65I G'GTAC_C Cuts at: 2920 2920 Size: 5599 AclI AA'CG_TT Cuts at: 1563 3726 4099 1563 Size: 2163 373 3063 Fragments arranged by size: 3063 2163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3662 5046 5314 1632 Size: 2030 1384 268 1917 Fragments arranged by size: 2030 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5402 AflIII A'CryG_T Cuts at: 1203 2538 4498 5475 1203 Size: 1335 1960 977 1327 Fragments arranged by size: 1960 1335 1327 977 AgeI A'CCGG_T Cuts at: 4430 4430 Size: 5599 AhdI GACnn_n'nnGTC Cuts at: 4328 4328 Size: 5599 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2157 2738 1184 Size: 973 581 4045 Fragments arranged by size: 4045 973 581 AlwNI CAG_nnn'CTG Cuts at: 3342 4914 3342 Size: 1572 4027 ApaLI G'TGCA_C Cuts at: 3656 4812 3656 Size: 1156 4443 ApoI r'AATT_y Cuts at: 1493 2462 2908 3134 3286 5395 1493 Size: 969 446 226 152 2109 1697 Fragments arranged by size: 2109 1697 969 446 226 152 AseI AT'TA_AT Cuts at: 160 1032 4153 160 Size: 872 3121 1606 Fragments arranged by size: 3121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 AvaI C'yCGr_G Cuts at: 1870 1943 2924 1870 Size: 73 981 4545 Fragments arranged by size: 4545 981 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2566 2599 2566 Size: 33 5566 BamHI G'GATC_C Cuts at: 2614 2929 3296 2614 Size: 315 367 4917 Fragments arranged by size: 4917 367 315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2920 4375 618 Size: 325 217 627 579 554 1455 1842 Fragments arranged by size: 1842 1455 627 579 554 325 217 BanII G_rGCy'C Cuts at: 1391 2918 1391 Size: 1527 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3790 3824 1903 Size: 34 1853 34 3678 Fragments arranged by size: 3678 1853 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3491 4701 3491 Size: 1210 4389 BclI T'GATC_A Cuts at: 1548 1548 Size: 5599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2375 4210 136 Size: 107 122 71 631 605 703 1835 1525 Fragments arranged by size: 1835 1525 703 631 605 122 107 71 BglII A'GATC_T Cuts at: 2295 2493 5594 2295 Size: 198 3101 2300 Fragments arranged by size: 3101 2300 198 BlpI GC'TnA_GC Cuts at: 3016 3016 Size: 5599 Bme1580I G_kGCm'C Cuts at: 1790 3660 4816 1790 Size: 1870 1156 2573 Fragments arranged by size: 2573 1870 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5599 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2320 3330 4288 Size: 416 53 83 399 24 903 1010 958 Cuts at: 4288 442 Size: 1753 Fragments arranged by size: 1753 1010 958 903 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2469 4259 1239 Size: 1230 1790 2579 Fragments arranged by size: 2579 1790 1230 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3724 4589 4887 3724 Size: 865 298 4436 Fragments arranged by size: 4436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4262 882 Size: 617 2763 2219 Fragments arranged by size: 2763 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3390 493 Size: 711 2186 2702 Fragments arranged by size: 2702 2186 711 BsaBI GATnn'nnATC Cuts at: 5593 5593 Size: 5599 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3406 3788 4423 275 Size: 53 83 186 936 1873 382 635 1451 Fragments arranged by size: 1873 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2611 4032 4430 4704 4851 5262 2611 Size: 1421 398 274 147 411 2948 Fragments arranged by size: 2948 1421 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5374 Fragments arranged by size: 5374 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5599 BseYI C'CCAG_C Cuts at: 4802 5298 4802 Size: 496 5103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2567 1163 Size: 573 402 429 4195 Fragments arranged by size: 4195 573 429 402 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3049 3810 4838 1540 Size: 275 186 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3660 3745 4816 3660 Size: 85 1071 4443 Fragments arranged by size: 4443 1071 85 BsmI GAATG_Cn' Cuts at: 2610 3110 3203 2610 Size: 500 93 5006 Fragments arranged by size: 5006 500 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2918 3660 3745 4816 1391 Size: 399 1128 742 85 1071 2174 Fragments arranged by size: 2174 1128 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2611 5262 2611 Size: 2651 2948 BspHI T'CATG_A Cuts at: 3488 5218 3488 Size: 1730 3869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2036 2104 2954 844 Size: 1192 68 850 3489 Fragments arranged by size: 3489 1192 850 68 BsrBI CCG'CTC Cuts at: 1394 1823 2095 3486 1394 Size: 429 272 1391 3507 Fragments arranged by size: 3507 1391 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 4094 4268 65 Size: 1183 2846 174 1396 Fragments arranged by size: 2846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4243 4430 1700 Size: 110 2433 187 2869 Fragments arranged by size: 2869 2433 187 110 BsrGI T'GTAC_A Cuts at: 96 2754 96 Size: 2658 2941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5599 BssSI C'ACGA_G Cuts at: 2790 3659 4671 2790 Size: 869 1012 3718 Fragments arranged by size: 3718 1012 869 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5599 BstBI TT'CG_AA Cuts at: 4403 4403 Size: 5599 BstEII G'GTnAC_C Cuts at: 1184 2222 1184 Size: 1038 4561 BstYI r'GATC_y Cuts at: 1972 2295 2493 2614 2929 3296 3681 3698 5150 Size: 323 198 121 315 367 385 17 1452 Cuts at: 5150 5236 5248 5594 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 323 315 198 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5047 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4860 Fragments arranged by size: 4860 683 56 BtsI GCAGTG_nn' Cuts at: 2139 2592 2664 3115 3909 3929 2139 Size: 453 72 451 794 20 3809 Fragments arranged by size: 3809 794 453 451 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5564 DraI TTT'AAA Cuts at: 2903 2903 Size: 5599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2177 4606 809 Size: 507 861 2429 1802 Fragments arranged by size: 2429 1802 861 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2229 3046 Size: 54 1425 50 275 18 225 174 817 Cuts at: 3046 3935 5564 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 817 275 225 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3046 1537 Size: 275 1234 4090 Fragments arranged by size: 4090 1234 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3529 1353 Size: 2176 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2794 4186 4560 4706 387 Size: 1064 138 1205 1392 374 146 1280 Fragments arranged by size: 1392 1280 1205 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2469 3662 4259 5046 5314 1239 Size: 393 837 1193 597 787 268 1524 Fragments arranged by size: 1524 1193 837 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2518 2518 Size: 5599 EcoRI G'AATT_C Cuts at: 2462 2908 3286 2462 Size: 446 378 4775 Fragments arranged by size: 4775 446 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2314 2314 Size: 5599 FspI TGC'GCA Cuts at: 4105 4105 Size: 5599 HaeII r_GCGC'y Cuts at: 1678 2087 2338 4746 1678 Size: 409 251 2408 2531 Fragments arranged by size: 2531 2408 409 251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2674 2706 4240 4272 4314 Size: 32 135 32 1102 32 1534 32 42 Cuts at: 4314 4346 1373 Size: 32 2626 Fragments arranged by size: 2626 1534 1102 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2682 2943 3189 669 Size: 988 281 744 261 246 3079 Fragments arranged by size: 3079 988 744 281 261 246 HindIII A'AGCT_T Cuts at: 748 2808 748 Size: 2060 3539 HpaI GTT'AAC Cuts at: 3189 3189 Size: 5599 KpnI G_GTAC'C Cuts at: 2924 2924 Size: 5599 MfeI C'AATT_G Cuts at: 3198 3198 Size: 5599 MluI A'CGCG_T Cuts at: 5475 5475 Size: 5599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4713 4897 1305 Size: 3408 184 2007 Fragments arranged by size: 3408 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2231 10 Size: 54 1425 343 399 3378 Fragments arranged by size: 3378 1425 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2386 2720 3557 3916 4075 518 Size: 648 1220 334 837 359 159 2042 Fragments arranged by size: 2042 1220 837 648 359 334 159 MspA1I CmG'CkG Cuts at: 1606 2018 2432 2643 3015 3692 4840 5085 1606 Size: 412 414 211 372 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 414 412 372 245 211 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5047 NdeI CA'TA_TG Cuts at: 387 387 Size: 5599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5599 NotI GC'GGCC_GC Cuts at: 3046 3046 Size: 5599 NspI r_CATG'y Cuts at: 1132 1229 2126 2957 4502 1132 Size: 97 897 831 1545 2229 Fragments arranged by size: 2229 1545 897 831 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5599 PciI A'CATG_T Cuts at: 4498 4498 Size: 5599 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5599 PmeI GTTT'AAAC Cuts at: 2903 2903 Size: 5599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 5181 5213 3668 Size: 32 1481 32 4054 Fragments arranged by size: 4054 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5599 PsiI TTA'TAA Cuts at: 3169 3169 Size: 5599 PstI C_TGCA'G Cuts at: 830 2115 2146 2951 830 Size: 1285 31 805 3478 Fragments arranged by size: 3478 1285 805 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5599 PvuII CAG'CTG Cuts at: 2432 2643 2432 Size: 211 5388 SalI G'TCGA_C Cuts at: 1936 2941 1936 Size: 1005 4594 SbfI CC_TGCA'GG Cuts at: 2951 2951 Size: 5599 ScaI AGT'ACT Cuts at: 3847 5559 3847 Size: 1712 3887 SfcI C'TryA_G Cuts at: 826 1045 2111 2142 2947 4082 4763 4954 826 Size: 219 1066 31 805 1135 681 191 1471 Fragments arranged by size: 1471 1135 1066 805 681 219 191 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5599 SmaI CCC'GGG Cuts at: 1872 2926 1872 Size: 1054 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3703 4604 4866 820 Size: 197 926 1760 901 262 1553 Fragments arranged by size: 1760 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3390 493 Size: 2897 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5599 SphI G_CATG'C Cuts at: 1132 2126 2957 1132 Size: 994 831 3774 Fragments arranged by size: 3774 994 831 SspI AAT'ATT Cuts at: 5 52 3523 5 Size: 47 3471 2081 Fragments arranged by size: 3471 2081 47 StuI AGG'CCT Cuts at: 1517 2472 1517 Size: 955 4644 StyI C'CwwG_G Cuts at: 513 1065 2031 2190 2527 3038 513 Size: 552 966 159 337 511 3074 Fragments arranged by size: 3074 966 552 511 337 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2294 2840 3626 3643 3796 3981 Size: 1255 690 24 546 786 17 153 185 Cuts at: 3981 4426 325 Size: 445 1498 Fragments arranged by size: 1498 1255 786 690 546 445 185 153 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 2892 3845 Size: 274 80 33 51 1443 777 138 953 Cuts at: 3845 5557 96 Size: 1712 138 Fragments arranged by size: 1712 1443 953 777 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5559 5591 5559 Size: 32 5567 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5599 XbaI T'CTAG_A Cuts at: 2935 2935 Size: 5599 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2038 Size: 5599 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5599 XmaI C'CCGG_G Cuts at: 1870 2924 1870 Size: 1054 4545 XmnI GAAnn'nnTTC Cuts at: 3728 3728 Size: 5599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3407 276 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI