(Linear) (Six-base) MAPSORT of: KIEE2309.seq Check: 372 from: 1 to: 3771 KIEE2309.orf With 182 enzymes: SgfI * July 3, 2012 17:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1253 1648 2268 3771 Size: 1253 395 620 1503 Fragments arranged by size: 1503 1253 620 395 AccI GT'mk_AC Cuts at: 0 3143 3771 Size: 3143 628 Acc65I G'GTAC_C Cuts at: 0 488 3771 Size: 488 3283 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1394 1849 2558 2612 3771 Size: 1394 455 709 54 1159 Fragments arranged by size: 1394 1159 709 455 54 AflIII A'CryG_T Cuts at: 0 2336 3771 Size: 2336 1435 AhdI GACnn_n'nnGTC Cuts at: 0 198 3771 Size: 198 3573 AleI CACnn'nnGTG Cuts at: 0 811 3712 3771 Size: 811 2901 59 Fragments arranged by size: 2901 811 59 AlwNI CAG_nnn'CTG Cuts at: 0 837 3457 3771 Size: 837 2620 314 Fragments arranged by size: 2620 837 314 AvaI C'yCGr_G Cuts at: 0 108 298 595 1096 1548 1586 2048 3322 Size: 108 190 297 501 452 38 462 1274 Cuts at: 3322 3429 3771 Size: 107 342 Fragments arranged by size: 1274 501 462 452 342 297 190 108 107 38 BamHI G'GATC_C Cuts at: 0 1582 1795 3771 Size: 1582 213 1976 Fragments arranged by size: 1976 1582 213 BanI G'GyrC_C Cuts at: 0 403 488 1042 1076 1089 1331 1651 3663 Size: 403 85 554 34 13 242 320 2012 Cuts at: 3663 3771 Size: 108 Fragments arranged by size: 2012 554 403 320 242 108 85 34 13 BanII G_rGCy'C Cuts at: 0 920 1014 1362 1483 1501 1519 1603 1618 Size: 920 94 348 121 18 18 84 15 Cuts at: 1618 2254 2419 2452 2642 3534 3567 3771 Size: 636 165 33 190 892 33 204 Fragments arranged by size: 920 892 636 348 204 190 165 121 94 84 33 33 18 18 15 BbeI G_GCGC'C Cuts at: 0 407 1046 3771 Size: 407 639 2725 Fragments arranged by size: 2725 639 407 BbsI GAAGACnn'nnnn_ Cuts at: 0 45 1175 1242 1361 3252 3771 Size: 45 1130 67 119 1891 519 Fragments arranged by size: 1891 1130 519 119 67 45 BbvCI CC'TCA_GC Cuts at: 0 3345 3388 3771 Size: 3345 43 383 Fragments arranged by size: 3345 383 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1020 1054 3771 Size: 1020 34 2717 Fragments arranged by size: 2717 1020 34 BclI T'GATC_A Cuts at: 0 440 3771 Size: 440 3331 BfrBI ATG'CAT Cuts at: 0 960 3771 Size: 960 2811 BglI GCCn_nnn'nGGC Cuts at: 0 376 617 658 1254 2109 3771 Size: 376 241 41 596 855 1662 Fragments arranged by size: 1662 855 596 376 241 41 BglII A'GATC_T Cuts at: 0 2928 3771 Size: 2928 843 BlpI GC'TnA_GC Cuts at: 0 580 748 1305 3771 Size: 580 168 557 2466 Fragments arranged by size: 2466 580 557 168 Bme1580I G_kGCm'C Cuts at: 0 275 737 1654 1667 3771 Size: 275 462 917 13 2104 Fragments arranged by size: 2104 917 462 275 13 BmgBI CAC'GTC Cuts at: 0 1324 3736 3771 Size: 1324 2412 35 Fragments arranged by size: 2412 1324 35 BmrI ACTGGGnnnn_n' Cuts at: 0 2553 3771 Size: 2553 1218 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1129 2322 3771 Size: 1129 1193 1449 Fragments arranged by size: 1449 1193 1129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1209 1518 1712 1748 1940 2124 2372 2621 Size: 1209 309 194 36 192 184 248 249 Cuts at: 2621 3581 3771 Size: 960 190 Fragments arranged by size: 1209 960 309 249 248 194 192 190 184 36 Bpu10I CC'TnA_GC Cuts at: 0 838 1482 1500 1617 1707 1743 1779 3345 Size: 838 644 18 117 90 36 36 1566 Cuts at: 3345 3388 3771 Size: 43 383 Fragments arranged by size: 1566 838 644 383 117 90 43 36 36 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2798 3771 Size: 2798 973 BsaI GGTCTCn'nnnn_ Cuts at: 0 1680 2655 3323 3771 Size: 1680 975 668 448 Fragments arranged by size: 1680 975 668 448 BsaAI yAC'GTr Cuts at: 0 2100 2950 3714 3771 Size: 2100 850 764 57 Fragments arranged by size: 2100 850 764 57 BsaHI Gr'CG_yC Cuts at: 0 311 404 1043 3249 3771 Size: 311 93 639 2206 522 Fragments arranged by size: 2206 639 522 311 93 BsaWI w'CCGG_w Cuts at: 0 3636 3771 Size: 3636 135 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 775 805 2787 2817 3771 Size: 775 30 1982 30 954 Fragments arranged by size: 1982 954 775 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 223 796 1889 1892 1997 2153 3771 Size: 223 573 1093 3 105 156 1618 Fragments arranged by size: 1618 1093 573 223 156 105 3 BseYI C'CCAG_C Cuts at: 0 315 729 1204 1312 1317 3184 3771 Size: 315 414 475 108 5 1867 587 Fragments arranged by size: 1867 587 475 414 315 108 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 1016 1225 2063 3771 Size: 71 945 209 838 1708 Fragments arranged by size: 1708 945 838 209 71 BsiEI CG_ry'CG Cuts at: 0 122 395 653 3771 Size: 122 273 258 3118 Fragments arranged by size: 3118 273 258 122 BsiHKAI G_wGCw'C Cuts at: 0 293 920 1362 1636 2419 2452 3771 Size: 293 627 442 274 783 33 1319 Fragments arranged by size: 1319 783 627 442 293 274 33 BsmI GAATG_Cn' Cuts at: 0 2002 3771 Size: 2002 1769 Bsp1286I G_dGCh'C Cuts at: 0 275 293 737 920 1014 1362 1483 1501 Size: 275 18 444 183 94 348 121 18 Cuts at: 1501 1519 1603 1618 1636 1654 1667 2254 2419 Size: 18 84 15 18 18 13 587 165 Cuts at: 2419 2452 2642 3534 3567 3771 Size: 33 190 892 33 204 Fragments arranged by size: 892 587 444 348 275 204 190 183 165 121 94 84 33 33 18 18 18 18 18 15 13 BspEI T'CCGG_A Cuts at: 0 3636 3771 Size: 3636 135 BspHI T'CATG_A Cuts at: 0 2634 3771 Size: 2634 1137 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 224 260 696 881 918 1250 1253 1648 Size: 224 36 436 185 37 332 3 395 Cuts at: 1648 2268 3771 Size: 620 1503 Fragments arranged by size: 1503 620 436 395 332 224 185 37 36 3 BsrBI CCG'CTC Cuts at: 0 420 752 1095 1273 3634 3771 Size: 420 332 343 178 2361 137 Fragments arranged by size: 2361 420 343 332 178 137 BsrDI GCAATG_nn' Cuts at: 0 500 3027 3077 3771 Size: 500 2527 50 694 Fragments arranged by size: 2527 694 500 50 BsrFI r'CCGG_y Cuts at: 0 648 1284 2356 3544 3771 Size: 648 636 1072 1188 227 Fragments arranged by size: 1188 1072 648 636 227 BssHII G'CGCG_C Cuts at: 0 629 3771 Size: 629 3142 BstAPI GCAn_nnn'nTGC Cuts at: 0 3586 3771 Size: 3586 185 BstEII G'GTnAC_C Cuts at: 0 87 3771 Size: 87 3684 BstXI CCAn_nnnn'nTGG Cuts at: 0 729 1690 1726 2831 3771 Size: 729 961 36 1105 940 Fragments arranged by size: 1105 961 940 729 36 BstYI r'GATC_y Cuts at: 0 1582 1795 1837 2030 2928 3771 Size: 1582 213 42 193 898 843 Fragments arranged by size: 1582 898 843 213 193 42 Bsu36I CC'TnA_GG Cuts at: 0 1147 2387 3771 Size: 1147 1240 1384 Fragments arranged by size: 1384 1240 1147 BtgI C'CryG_G Cuts at: 0 170 371 430 1737 3083 3771 Size: 170 201 59 1307 1346 688 Fragments arranged by size: 1346 1307 688 201 170 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 547 3771 Size: 547 3224 BtsI GCAGTG_nn' Cuts at: 0 89 3771 Size: 89 3682 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2112 2147 3771 Size: 2112 35 1624 Fragments arranged by size: 2112 1624 35 DrdI GACnn_nn'nnGTC Cuts at: 0 141 220 784 2530 3771 Size: 141 79 564 1746 1241 Fragments arranged by size: 1746 1241 564 141 79 EaeI y'GGCC_r Cuts at: 0 173 392 539 650 815 1286 1491 1509 Size: 173 219 147 111 165 471 205 18 Cuts at: 1509 1524 1608 1644 1680 1716 1734 2120 2983 Size: 15 84 36 36 36 18 386 863 Cuts at: 2983 3771 Size: 788 Fragments arranged by size: 863 788 471 386 219 205 173 165 147 111 84 36 36 36 18 18 15 EagI C'GGCC_G Cuts at: 0 392 650 3771 Size: 392 258 3121 Fragments arranged by size: 3121 392 258 EarI CTCTTCn'nnn_ Cuts at: 0 200 2403 3029 3227 3771 Size: 200 2203 626 198 544 Fragments arranged by size: 2203 626 544 200 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 691 3459 3771 Size: 691 2768 312 Fragments arranged by size: 2768 691 312 EcoICRI GAG'CTC Cuts at: 0 918 1360 2417 2450 3771 Size: 918 442 1057 33 1321 Fragments arranged by size: 1321 1057 918 442 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1209 1394 1518 1712 1748 1849 1940 2124 Size: 1209 185 124 194 36 101 91 184 Cuts at: 2124 2372 2558 2612 2621 3581 3771 Size: 248 186 54 9 960 190 Fragments arranged by size: 1209 960 248 194 190 186 185 184 124 101 91 54 36 9 EcoO109I rG'GnC_Cy Cuts at: 0 213 1121 1723 2053 2517 2547 3771 Size: 213 908 602 330 464 30 1224 Fragments arranged by size: 1224 908 602 464 330 213 30 HaeII r_GCGC'y Cuts at: 0 282 407 609 1046 1194 2193 3771 Size: 282 125 202 437 148 999 1578 Fragments arranged by size: 1578 999 437 282 202 148 125 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 184 216 535 567 831 863 2596 2628 Size: 184 32 319 32 264 32 1733 32 Cuts at: 2628 2684 2716 3281 3313 3457 3489 3771 Size: 56 32 565 32 144 32 282 Fragments arranged by size: 1733 565 319 282 264 184 144 56 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1055 1977 2766 3144 3771 Size: 1055 922 789 378 627 Fragments arranged by size: 1055 922 789 627 378 HpaI GTT'AAC Cuts at: 0 1055 3771 Size: 1055 2716 KasI G'GCGC_C Cuts at: 0 403 1042 3771 Size: 403 639 2729 Fragments arranged by size: 2729 639 403 KpnI G_GTAC'C Cuts at: 0 492 3771 Size: 492 3279 MfeI C'AATT_G Cuts at: 0 1473 3771 Size: 1473 2298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 533 2392 3334 3771 Size: 533 1859 942 437 Fragments arranged by size: 1859 942 533 437 MscI TGG'CCA Cuts at: 0 817 1511 1526 1610 1646 1682 1718 1736 Size: 817 694 15 84 36 36 36 18 Cuts at: 1736 2122 2985 3771 Size: 386 863 786 Fragments arranged by size: 863 817 786 694 386 84 36 36 36 18 15 MslI CAynn'nnrTG Cuts at: 0 811 1465 2233 2341 3712 3771 Size: 811 654 768 108 1371 59 Fragments arranged by size: 1371 811 768 654 108 59 MspA1I CmG'CkG Cuts at: 0 58 67 373 388 432 614 670 1016 Size: 58 9 306 15 44 182 56 346 Cuts at: 1016 1918 3295 3454 3482 3622 3628 3771 Size: 902 1377 159 28 140 6 143 Fragments arranged by size: 1377 902 346 306 182 159 143 140 58 56 44 28 15 9 6 NaeI GCC'GGC Cuts at: 0 650 1286 3771 Size: 650 636 2485 Fragments arranged by size: 2485 650 636 NarI GG'CG_CC Cuts at: 0 404 1043 3771 Size: 404 639 2728 Fragments arranged by size: 2728 639 404 NcoI C'CATG_G Cuts at: 0 1737 3083 3771 Size: 1737 1346 688 Fragments arranged by size: 1737 1346 688 NgoMIV G'CCGG_C Cuts at: 0 648 1284 3771 Size: 648 636 2487 Fragments arranged by size: 2487 648 636 NotI GC'GGCC_GC Cuts at: 0 392 3771 Size: 392 3379 NruI TCG'CGA Cuts at: 0 23 532 3771 Size: 23 509 3239 Fragments arranged by size: 3239 509 23 NsiI A_TGCA'T Cuts at: 0 962 3771 Size: 962 2809 NspI r_CATG'y Cuts at: 0 960 1464 2340 3771 Size: 960 504 876 1431 Fragments arranged by size: 1431 960 876 504 PasI CC'CwG_GG Cuts at: 0 722 1422 3771 Size: 722 700 2349 Fragments arranged by size: 2349 722 700 PciI A'CATG_T Cuts at: 0 2336 3771 Size: 2336 1435 PflMI CCAn_nnn'nTGG Cuts at: 0 1918 2411 3771 Size: 1918 493 1360 Fragments arranged by size: 1918 1360 493 PfoI T'CCnGG_A Cuts at: 0 150 909 2349 3771 Size: 150 759 1440 1422 Fragments arranged by size: 1440 1422 759 150 PmlI CAC'GTG Cuts at: 0 2950 3714 3771 Size: 2950 764 57 Fragments arranged by size: 2950 764 57 PpuMI rG'GwC_Cy Cuts at: 0 213 1121 1723 2053 2547 3771 Size: 213 908 602 330 494 1224 Fragments arranged by size: 1224 908 602 494 330 213 PspXI vC'TCGA_Gb Cuts at: 0 1096 2048 3771 Size: 1096 952 1723 Fragments arranged by size: 1723 1096 952 PstI C_TGCA'G Cuts at: 0 384 1341 3227 3372 3627 3771 Size: 384 957 1886 145 255 144 Fragments arranged by size: 1886 957 384 255 145 144 PvuII CAG'CTG Cuts at: 0 1918 3454 3628 3771 Size: 1918 1536 174 143 Fragments arranged by size: 1918 1536 174 143 RsrII CG'GwC_CG Cuts at: 0 907 3771 Size: 907 2864 SacI G_AGCT'C Cuts at: 0 920 1362 2419 2452 3771 Size: 920 442 1057 33 1319 Fragments arranged by size: 1319 1057 920 442 33 SacII CC_GC'GG Cuts at: 0 374 433 3771 Size: 374 59 3338 Fragments arranged by size: 3338 374 59 SalI G'TCGA_C Cuts at: 0 3142 3771 Size: 3142 629 SanDI GG'GwC_CC Cuts at: 0 2547 3771 Size: 2547 1224 SapI GCTCTTCn'nnn_ Cuts at: 0 2403 3227 3771 Size: 2403 824 544 Fragments arranged by size: 2403 824 544 SfcI C'TryA_G Cuts at: 0 380 1026 1337 3011 3209 3223 3368 3623 Size: 380 646 311 1674 198 14 145 255 Cuts at: 3623 3771 Size: 148 Fragments arranged by size: 1674 646 380 311 255 198 148 145 14 SfoI GGC'GCC Cuts at: 0 405 1044 3771 Size: 405 639 2727 Fragments arranged by size: 2727 639 405 SmaI CCC'GGG Cuts at: 0 3431 3771 Size: 3431 340 SmlI C'TyrA_G Cuts at: 0 298 595 1096 2048 2813 3322 3771 Size: 298 297 501 952 765 509 449 Fragments arranged by size: 952 765 509 501 449 298 297 StyI C'CwwG_G Cuts at: 0 1527 1737 3083 3771 Size: 1527 210 1346 688 Fragments arranged by size: 1527 1346 688 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 529 895 1218 1326 3771 Size: 529 366 323 108 2445 Fragments arranged by size: 2445 529 366 323 108 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2185 2217 3771 Size: 2185 32 1554 Fragments arranged by size: 2185 1554 32 Tth111I GACn'n_nGTC Cuts at: 0 138 2761 3771 Size: 138 2623 1010 Fragments arranged by size: 2623 1010 138 XbaI T'CTAG_A Cuts at: 0 1636 3771 Size: 1636 2135 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1212 1519 1744 3771 Size: 1212 307 225 2027 Fragments arranged by size: 2027 1212 307 225 XhoI C'TCGA_G Cuts at: 0 298 595 1096 2048 3322 3771 Size: 298 297 501 952 1274 449 Fragments arranged by size: 1274 952 501 449 298 297 XmaI C'CCGG_G Cuts at: 0 3429 3771 Size: 3429 342 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AleI AlwNI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspXI PstI PvuII RsrII SacI SacII SalI SanDI SapI SfcI SfoI SmaI SmlI StyI TaqII TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI BmtI BsaBI BsiWI BsmBI BsrGI BssSI BstBI BstZ17I ClaI DraI DraIII EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII MluI NdeI NheI PacI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XmnI ZraI