################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII ItaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI StyI SwaI TseI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 133 GTCTAC GT^[AC][GT]AC AflIII 582 ACGTGT A^C[GA][CT]GT AseI 1089 ATTAAT AT^TAAT AsnI 1089 ATTAAT AT^TAAT AvaII 722 GGACC G^G[AT]CC BalI 413 TGGCCA TGG^CCA BamHI 488 GGATCC G^GATCC BglII 335 AGATCT A^GATCT BsiYI 58 CCCAGGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 58 CCCAGGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 349 GCATTC GAATGC(1/-1) Bsp1407I 268 TGTACA T^GTACA BspMI 559 ACCTGC ACCTGC(4/8) BsrDI 68 GCAATG GCAATG(2/0) BsrGI 268 TGTACA T^GTACA BsrI 308 ACTGG ACTGG(1/-1) CfoI 825 GCGC GCG^C Cfr13I 722 GGACC G^G[ACGT]CC DraI 280 TTTAAA TTT^AAA DraIII 885 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 413 TGGCCA [CT]^GGCC[GA] EcoRI 459 GAATTC G^AATTC HhaI 825 GCGC GCG^C HinP1I 825 GCGC G^CGC HincII 541 GTCAAC GT[CT]^[GA]AC HindII 541 GTCAAC GT[CT]^[GA]AC MaeII 582 ACGT A^CGT MluNI 413 TGGCCA TGG^CCA MscI 413 TGGCCA TGG^CCA MwoI 563 GCTTTGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 760 CATATG CA^TATG NspI 374 GCATGC [GA]CATG^[CT] PleI 991 GAGTC GAGTC(4/5) PshBI 1089 ATTAAT AT^TAAT SapI 668 GCTCTTC GCTCTTC(1/4) Sau96I 722 GGACC G^G[ACGT]CC ScaI 738 AGTACT AGT^ACT SfcI 135 CTACAG C^T[GA][CT]AG SphI 374 GCATGC GCATG^C SspBI 268 TGTACA T^GTACA TaqI 432 TCGA T^CGA TthHB8I 432 TCGA T^CGA XcmI 306 CCACTGGCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 42 AAATTC [GA]^AATT[CT] 459 GAATTC ApoI 42 AAATTC [GA]^AATT[CT] 459 GAATTC Asp700I 946 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 957 GAAGTCCTTC BbsI 591 GAAGAC GAAGAC(2/6) 187 GTCTTC BclI 749 TGATCA T^GATCA 1062 TGATCA BpuAI 591 GAAGAC GAAGAC(2/6) 187 GTCTTC BsaJI 58 CCCAGG C^C[ACGT][ACGT]GG 818 CCCTGG BsmFI 721 GGGAC GGGAC(10/14) 993 GTCCC BstYI 335 AGATCT [GA]^GATC[CT] 488 GGATCC FbaI 749 TGATCA T^GATCA 1062 TGATCA HaeIII 311 GGCC GG^CC 414 GGCC MaeIII 5 GTGAC ^GT[ACGT]AC 320 GTGAC MflI 335 AGATCT [GA]^GATC[CT] 488 GGATCC MslI 201 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 981 CATCAGAATG NlaIV 488 GGATCC GG[ACGT]^[ACGT]CC 721 GGGACC Tsp45I 5 GTGAC ^GT[GC]AC 320 GTGAC XhoII 335 AGATCT [GA]^GATC[CT] 488 GGATCC XmnI 946 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 957 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AfaI AluI AlwI BfaI BsmAI BssKI BstNI Cac8I DdeI DpnII EarI EcoRII HinfI HphI Ksp632I MaeI MboI MboII MnlI MseI MvaI NdeII NlaIII RsaI Sau3AI ScrFI SspI TfiI Tru9I Tsp509I TspRI