(Circular) (Six-base) MAPSORT of: pFN21AE9513.seq Check: 5270 from: 1 to: 5800 pFN21AE9513 5800 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2393 3033 2393 Size: 640 5160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2950 3610 278 Size: 53 83 186 936 1414 660 2468 Fragments arranged by size: 2468 1414 936 660 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2587 2598 3863 5247 5515 1632 Size: 955 11 1265 1384 268 1917 Fragments arranged by size: 1917 1384 1265 955 268 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 2223 4699 5676 1203 Size: 1020 2476 977 1327 Fragments arranged by size: 2476 1327 1020 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2411 1184 Size: 1227 4573 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 2090 Size: 32 5768 AlwNI CAG_nnn'CTG Cuts at: 2057 2705 3072 3543 5115 2057 Size: 648 367 471 1572 2742 Fragments arranged by size: 2742 1572 648 471 367 ApaLI G'TGCA_C Cuts at: 2396 2597 3857 5013 2396 Size: 201 1260 1156 3183 Fragments arranged by size: 3183 1260 1156 201 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3125 1870 Size: 73 1182 4545 Fragments arranged by size: 4545 1182 73 AvrII C'CTAG_G Cuts at: 2038 3083 2038 Size: 1045 4755 BamHI G'GATC_C Cuts at: 2908 3130 3497 2908 Size: 222 367 5211 Fragments arranged by size: 5211 367 222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3121 4576 618 Size: 325 217 627 1334 1455 1842 Fragments arranged by size: 1842 1455 1334 627 325 217 BanII G_rGCy'C Cuts at: 1391 3119 1391 Size: 1728 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2709 928 Size: 971 810 4019 Fragments arranged by size: 4019 971 810 BbvCI CC'TCA_GC Cuts at: 2045 2045 Size: 5800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2460 4411 136 Size: 107 122 71 631 605 788 1951 1525 Fragments arranged by size: 1951 1525 788 631 605 122 107 71 BglII A'GATC_T Cuts at: 2322 5795 2322 Size: 3473 2327 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2400 2434 2511 2601 2640 3861 5017 1790 Size: 610 34 77 90 39 1221 1156 2573 Fragments arranged by size: 2573 1221 1156 610 90 77 39 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2940 3531 4489 Size: 416 53 83 399 24 1523 591 958 Cuts at: 4489 442 Size: 1753 Fragments arranged by size: 1753 1523 958 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4460 1239 Size: 3221 2579 Bpu10I CC'TnA_GC Cuts at: 2045 2045 Size: 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2947 3607 3989 4624 Size: 53 83 186 936 1414 660 382 635 Cuts at: 4624 275 Size: 1451 Fragments arranged by size: 1451 1414 936 660 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2937 2967 1178 Size: 30 165 30 1534 30 4011 Fragments arranged by size: 4011 1534 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5800 BseYI C'CCAG_C Cuts at: 2146 2439 2982 5003 5499 2146 Size: 293 543 2021 496 2447 Fragments arranged by size: 2447 2021 543 496 293 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2691 1163 Size: 573 955 4272 Fragments arranged by size: 4272 955 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 2601 2919 3861 3946 5017 2400 Size: 201 318 942 85 1071 3183 Fragments arranged by size: 3183 1071 942 318 201 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2400 2434 2511 2601 2640 2919 3119 Size: 399 610 34 77 90 39 279 200 Cuts at: 3119 3861 3946 5017 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 610 399 279 200 90 85 77 39 34 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2393 3033 3155 844 Size: 1549 640 122 3489 Fragments arranged by size: 3489 1549 640 122 BsrBI CCG'CTC Cuts at: 1394 1823 2730 3687 1394 Size: 429 907 957 3507 Fragments arranged by size: 3507 957 907 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2495 4444 4631 1700 Size: 110 685 1949 187 2869 Fragments arranged by size: 2869 1949 685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 2504 3860 4872 2504 Size: 1356 1012 3432 Fragments arranged by size: 3432 1356 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2175 2749 2824 2175 Size: 574 75 5151 Fragments arranged by size: 5151 574 75 BstYI r'GATC_y Cuts at: 1972 2033 2322 2908 3130 3497 3882 3899 5351 Size: 61 289 586 222 367 385 17 1452 Cuts at: 5351 5437 5449 5795 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 586 385 367 346 289 222 86 61 17 12 Bsu36I CC'TnA_GG Cuts at: 3027 3027 Size: 5800 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 2071 2374 3316 4110 4130 2071 Size: 303 942 794 20 3741 Fragments arranged by size: 3741 942 794 303 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DraIII CAC_nnn'GTG Cuts at: 2596 2596 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2214 2814 Size: 54 1425 50 275 18 177 207 600 Cuts at: 2814 2886 3051 3247 4136 5765 8 Size: 72 165 196 889 1629 43 Fragments arranged by size: 1629 1425 889 600 275 207 196 177 165 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2574 3007 3730 1353 Size: 833 388 433 723 3423 Fragments arranged by size: 3423 833 723 433 388 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2180 4387 4761 4907 387 Size: 1064 138 591 2207 374 146 1280 Fragments arranged by size: 2207 1280 1064 591 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2587 2598 3863 4460 5247 5515 1239 Size: 393 955 11 1265 597 787 268 1524 Fragments arranged by size: 1524 1265 955 787 597 393 268 11 EcoNI CCTnn'n_nnAGG Cuts at: 3026 3026 Size: 5800 EcoO109I rG'GnC_Cy Cuts at: 2054 2054 Size: 5800 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 4947 1678 Size: 3269 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2937 2969 4441 4473 4515 Size: 32 135 32 1365 32 1472 32 42 Cuts at: 4515 4547 1373 Size: 32 2626 Fragments arranged by size: 2626 1472 1365 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2857 3144 3390 669 Size: 988 281 919 287 246 3079 Fragments arranged by size: 3079 988 919 287 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2221 2805 4914 5098 1305 Size: 916 584 2109 184 2007 Fragments arranged by size: 2109 2007 916 584 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2816 2888 10 Size: 54 1425 343 177 807 72 2922 Fragments arranged by size: 2922 1425 807 343 177 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2393 2747 3758 4117 4276 518 Size: 648 1056 171 354 1011 359 159 2042 Fragments arranged by size: 2042 1056 1011 648 359 354 171 159 MspA1I CmG'CkG Cuts at: 1606 2901 3039 3216 3893 5041 5286 1606 Size: 1295 138 177 677 1148 245 2120 Fragments arranged by size: 2120 1295 1148 677 245 177 138 NaeI GCC'GGC Cuts at: 2497 2497 Size: 5800 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NgoMIV G'CCGG_C Cuts at: 2495 2495 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2227 3158 4703 1132 Size: 97 998 931 1545 2229 Fragments arranged by size: 2229 1545 998 931 97 PasI CC'CwG_GG Cuts at: 1348 2202 1348 Size: 854 4946 PciI A'CATG_T Cuts at: 2223 4699 2223 Size: 2476 3324 PflMI CCAn_nnn'nTGG Cuts at: 1108 2057 1108 Size: 949 4851 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2054 2054 Size: 5800 PshAI GACnn'nnGTC Cuts at: 1613 2549 1613 Size: 936 4864 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 2369 3044 3152 830 Size: 1539 675 108 3478 Fragments arranged by size: 3478 1539 675 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2901 3039 2901 Size: 138 5662 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SapI GCTCTTCn'nnn_ Cuts at: 2186 2186 Size: 5800 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 2844 4048 5760 2844 Size: 1204 1712 2884 Fragments arranged by size: 2884 1712 1204 SfcI C'TryA_G Cuts at: 826 1045 2365 3040 3148 4283 4964 5155 826 Size: 219 1320 675 108 1135 681 191 1471 Fragments arranged by size: 1471 1320 1135 681 675 219 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3904 4805 5067 820 Size: 197 926 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 3013 152 Size: 2861 2939 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2043 2463 1517 Size: 526 420 4854 Fragments arranged by size: 4854 526 420 StyI C'CwwG_G Cuts at: 513 1065 2004 2038 3083 3239 513 Size: 552 939 34 1045 156 3074 Fragments arranged by size: 3074 1045 939 552 156 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2436 3827 3844 3997 4182 4627 325 Size: 1255 856 1391 17 153 185 445 1498 Fragments arranged by size: 1498 1391 1255 856 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2012 2842 4046 Size: 274 80 33 51 1443 35 830 1204 Cuts at: 4046 5758 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1204 830 274 138 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2309 2809 2902 2309 Size: 500 93 5207 Fragments arranged by size: 5207 500 93 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2948 3608 276 Size: 53 83 186 936 1414 660 2468 Fragments arranged by size: 2468 1414 936 660 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI