Restriction map for
pFN21AE5666
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BalI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 625 AAATTT [GA]^AATT[CT]
AfaI 1176 GTAC GT^AC
AflIII 977 ACACGT A^C[GA][CT]GT
AlwNI 1400 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 625 AAATTT [GA]^AATT[CT]
AspI 16 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1273 GGCACC G^G[CT][GA]CC
BclI 854 TGATCA T^GATCA
BseRI 1168 GAGGAG GAGGAG(10/8)
BsgI 1418 CTGCAC GTGCAG(16/14)
BssSI 860 CTCGTG CACGAG(-5/-1)
BstAPI 5 GCAACAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI 421 GAAGAG CTCTTC(1/4)
FbaI 854 TGATCA T^GATCA
HgaI 258 GCGTC GACGC(5/10)
HphI 1430 TCACC GGTGA(8/7)
Ksp632I 421 GAAGAG CTCTTC(1/4)
MaeIII 517 GTCAC ^GT[ACGT]AC
MseI 152 TTAA T^TAA
NspI 1353 GCATGC [GA]CATG^[CT]
PflFI 16 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 521 CTGCAG CTGCA^G
RsaI 1176 GTAC GT^AC
ScaI 1175 AGTACT AGT^ACT
SmaI 104 CCCGGG CCC^GGG
SmlI 204 CTTGAG C^T[CT][GA]AG
SphI 1353 GCATGC GCATG^C
TaqI 598 TCGA T^CGA
Tru9I 152 TTAA T^TAA
Tsp45I 517 GTCAC ^GT[GC]AC
Tth111I 16 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 598 TCGA T^CGA
XcmI 1433 CCACCACCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 104 CCCGGG C^CCGGG
XmaI 104 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 104 CCCGGG C^[CT]CG[GA]G
1340 CCCGAG
BamHI 172 GGATCC G^GATCC
1214 GGATCC
BfaI 223 CTAG C^TAG
1123 CTAG
BsoBI 104 CCCGGG C^[CT]CG[GA]G
1340 CCCGAG
BsrBI 655 GAGCGG CCGCTC(-3/-3)
1296 GAGCGG
DsaI 400 CCATGG C^C[GA][CT]GG
1358 CCATGG
HapII 105 CCGG C^CGG
1325 CCGG
HpaII 105 CCGG C^CGG
1325 CCGG
MaeI 223 CTAG C^TAG
1123 CTAG
MspI 105 CCGG C^CGG
1325 CCGG
NcoI 400 CCATGG C^CATGG
1358 CCATGG
PflMI 1245 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1286 CCACACACTGG
SfaNI 394 GATGC GCATC(5/9)
38 GCATC
SfcI 521 CTGCAG C^T[GA][CT]AG
1316 CTGTAG
StuI 697 AGGCCT AGG^CCT
1470 AGGCCT
Tsp509I 327 AATT ^AATT
626 AATT
Van91I 1245 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1286 CCACACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BpmI BsaI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MaeII MboI MboII MflI MnlI
MslI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI StyI TfiI TseI TspRI
XhoII