(Linear) (Six-base) MAPSORT of: KIEE5666.seq Check: 1137 from: 1 to: 1560 KIEE5666.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 279 1019 1560 Size: 279 740 541 Fragments arranged by size: 740 541 279 AflIII A'CryG_T Cuts at: 0 977 1560 Size: 977 583 AleI CACnn'nnGTG Cuts at: 0 862 1560 Size: 862 698 AlwNI CAG_nnn'CTG Cuts at: 0 1405 1560 Size: 1405 155 ApoI r'AATT_y Cuts at: 0 625 1560 Size: 625 935 AvaI C'yCGr_G Cuts at: 0 104 1340 1560 Size: 104 1236 220 Fragments arranged by size: 1236 220 104 BamHI G'GATC_C Cuts at: 0 172 1214 1560 Size: 172 1042 346 Fragments arranged by size: 1042 346 172 BanI G'GyrC_C Cuts at: 0 1273 1560 Size: 1273 287 BclI T'GATC_A Cuts at: 0 854 1560 Size: 854 706 BmgBI CAC'GTC Cuts at: 0 18 1060 1560 Size: 18 1042 500 Fragments arranged by size: 1042 500 18 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1194 1560 Size: 1194 366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 936 950 1153 1314 1560 Size: 712 224 14 203 161 246 Fragments arranged by size: 712 246 224 203 161 14 Bpu10I CC'TnA_GC Cuts at: 0 1477 1560 Size: 1477 83 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 1560 Size: 225 1335 BsaI GGTCTCn'nnnn_ Cuts at: 0 226 520 543 671 736 1560 Size: 226 294 23 128 65 824 Fragments arranged by size: 824 294 226 128 65 23 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 668 698 1560 Size: 668 30 862 Fragments arranged by size: 862 668 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1183 1560 Size: 1183 377 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1403 1560 Size: 1403 157 BsrBI CCG'CTC Cuts at: 0 657 1298 1560 Size: 657 641 262 Fragments arranged by size: 657 641 262 BssSI C'ACGA_G Cuts at: 0 860 1560 Size: 860 700 BstAPI GCAn_nnn'nTGC Cuts at: 0 11 1560 Size: 11 1549 BstXI CCAn_nnnn'nTGG Cuts at: 0 317 1053 1113 1443 1560 Size: 317 736 60 330 117 Fragments arranged by size: 736 330 317 117 60 BstYI r'GATC_y Cuts at: 0 172 637 1214 1346 1560 Size: 172 465 577 132 214 Fragments arranged by size: 577 465 214 172 132 BtgI C'CryG_G Cuts at: 0 400 1358 1560 Size: 400 958 202 Fragments arranged by size: 958 400 202 BtsI GCAGTG_nn' Cuts at: 0 518 577 1560 Size: 518 59 983 Fragments arranged by size: 983 518 59 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 483 518 1560 Size: 483 35 1042 Fragments arranged by size: 1042 483 35 EarI CTCTTCn'nnn_ Cuts at: 0 416 1560 Size: 416 1144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 650 1560 Size: 650 910 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 279 712 936 950 1019 1153 1314 1560 Size: 279 433 224 14 69 134 161 246 Fragments arranged by size: 433 279 246 224 161 134 69 14 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 834 1560 Size: 834 726 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 117 149 716 748 843 875 1206 1238 Size: 117 32 567 32 95 32 331 32 Cuts at: 1238 1560 Size: 322 Fragments arranged by size: 567 331 322 117 95 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 162 962 1560 Size: 162 800 598 Fragments arranged by size: 800 598 162 MslI CAynn'nnrTG Cuts at: 0 315 439 862 1560 Size: 315 124 423 698 Fragments arranged by size: 698 423 315 124 NcoI C'CATG_G Cuts at: 0 400 1358 1560 Size: 400 958 202 Fragments arranged by size: 958 400 202 NspI r_CATG'y Cuts at: 0 1357 1560 Size: 1357 203 PasI CC'CwG_GG Cuts at: 0 1210 1560 Size: 1210 350 PflMI CCAn_nnn'nTGG Cuts at: 0 1251 1292 1560 Size: 1251 41 268 Fragments arranged by size: 1251 268 41 PsiI TTA'TAA Cuts at: 0 902 1560 Size: 902 658 PstI C_TGCA'G Cuts at: 0 525 1560 Size: 525 1035 ScaI AGT'ACT Cuts at: 0 1177 1560 Size: 1177 383 SfcI C'TryA_G Cuts at: 0 521 1316 1560 Size: 521 795 244 Fragments arranged by size: 795 521 244 SmaI CCC'GGG Cuts at: 0 106 1560 Size: 106 1454 SmlI C'TyrA_G Cuts at: 0 204 1560 Size: 204 1356 SphI G_CATG'C Cuts at: 0 1357 1560 Size: 1357 203 StuI AGG'CCT Cuts at: 0 699 1472 1560 Size: 699 773 88 Fragments arranged by size: 773 699 88 StyI C'CwwG_G Cuts at: 0 165 215 400 672 1106 1358 1560 Size: 165 50 185 272 434 252 202 Fragments arranged by size: 434 272 252 202 185 165 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 695 1560 Size: 695 865 TatI w'GTAC_w Cuts at: 0 1175 1560 Size: 1175 385 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 71 103 668 700 1560 Size: 71 32 565 32 860 Fragments arranged by size: 860 565 71 32 32 Tth111I GACn'n_nGTC Cuts at: 0 19 1560 Size: 19 1541 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1440 1560 Size: 1440 120 XmaI C'CCGG_G Cuts at: 0 104 1560 Size: 104 1456 Enzymes that do cut: AcuI AflIII AleI AlwNI ApoI AvaI BamHI BanI BclI BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsgI BsrBI BssSI BstAPI BstXI BstYI BtgI BtsI CspCI EarI EciI Eco57MI FalI Hin4I MmeI MslI NcoI NspI PasI PflMI PsiI PstI ScaI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI ZraI