################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeIII MamI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 413 ACGTGT A^C[GA][CT]GT AlwNI 521 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1039 GGGCCC GGGCC^C BalI 359 TGGCCA TGG^CCA BbsI 739 GAAGAC GAAGAC(2/6) BbvCI 204 GCTGAGG CCTCAGC(-5/-2) BglII 837 AGATCT A^GATCT BlpI 239 GCTCAGC GC^T[ACGT]AGC Bpu1102I 239 GCTCAGC GC^T[ACGT]AGC BpuAI 739 GAAGAC GAAGAC(2/6) BsaI 892 GAGACC GGTCTC(1/5) BsiYI 724 CCACTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 724 CCACTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 892 GAGAC GTCTC(1/5) BsmFI 830 GTCCC GGGAC(10/14) Bsp1407I 2 TGTACA T^GTACA BspHI 32 TCATGA T^CATGA BsrGI 2 TGTACA T^GTACA BstBI 161 TTCGAA TT^CGAA BstXI 362 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 239 GCTCAGC GC^T[ACGT]AGC EaeI 359 TGGCCA [CT]^GGCC[GA] EcoRV 501 GATATC GAT^ATC FauI 587 CCCGC CCCGC(4/6) MluNI 359 TGGCCA TGG^CCA MscI 359 TGGCCA TGG^CCA MseI 542 TTAA T^TAA MwoI 869 GCTTTGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspV 161 TTCGAA TT^CGAA PflMI 724 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 232 GACTC GAGTC(4/5) RcaI 32 TCATGA T^CATGA SfuI 161 TTCGAA TT^CGAA SspBI 2 TGTACA T^GTACA TaqI 162 TCGA T^CGA TfiI 1056 GAATC G^A[AT]TC Tru9I 542 TTAA T^TAA TthHB8I 162 TCGA T^CGA Van91I 724 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 614 GATCC GGATC(4/5) 624 GATCC AvaII 79 GGACC G^G[AT]CC 254 GGTCC BanII 60 GGGCTC G[GA]GC[CT]^C 1039 GGGCCC BmyI 60 GGGCTC G[AGT]GC[ACT]^C 1039 GGGCCC BsaJI 1034 CCCAGG C^C[ACGT][ACGT]GG 1035 CCAGGG BsgI 55 GTGCAG GTGCAG(16/14) 330 GTGCAG Bsp1286I 60 GGGCTC G[AGT]GC[ACT]^C 1039 GGGCCC BspMI 895 ACCTGC ACCTGC(4/8) 931 ACCTGC BsrDI 1003 CATTGC GCAATG(2/0) 437 GCAATG BssKI 756 CCAGG ^CC[ACGT]GG 1035 CCAGG BstNI 756 CCAGG CC^[AT]GG 1035 CCAGG Cac8I 200 GCAGGC GC[ACGT]^[ACGT]GC 572 GCTTGC DraII 1038 GGGGCCC [GA]G^G[ACGT]CC[CT] 1039 GGGCCCC EarI 375 GAAGAG CTCTTC(1/4) 442 GAAGAG EcoO109I 1038 GGGGCCC [GA]G^G[ACGT]CC[CT] 1039 GGGCCCC EcoRII 756 CCAGG ^CC[AT]GG 1035 CCAGG HinfI 232 GACTC G^A[ACGT]TC 1056 GAATC HphI 21 GGTGA GGTGA(8/7) 921 TCACC Ksp632I 375 GAAGAG CTCTTC(1/4) 442 GAAGAG MspA1I 49 CAGCTG C[AC]G^C[GT]G 936 CAGCTG MvaI 756 CCAGG CC^[AT]GG 1035 CCAGG NspI 674 ACATGC [GA]CATG^[CT] 991 GCATGT PstI 861 CTGCAG CTGCA^G 933 CTGCAG PvuII 49 CAGCTG CAG^CTG 936 CAGCTG ScrFI 756 CCAGG CC^[ACGT]GG 1035 CCAGG SfcI 861 CTGCAG C^T[GA][CT]AG 933 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AfaI AluI Asp700I BbvI BfaI BpmI BsrI BstF5I BstYI Cfr13I DdeI DpnII Fnu4HI FokI HaeIII ItaI MaeI MaeII MboI MboII MflI MnlI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I SfaNI TseI Tsp509I TspRI XhoII XmnI