(Circular) (Six-base) MAPSORT of: pFN21AE1836.seq Check: 8060 from: 1 to: 5767 pFN21AE1836 5767 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2945 2945 Size: 5767 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3110 1937 Size: 1173 4594 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2655 3830 5214 5482 1632 Size: 1023 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1175 1023 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 2418 4666 5643 1203 Size: 1215 2248 977 1327 Fragments arranged by size: 2248 1327 1215 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3025 1184 Size: 1841 3926 AlwNI CAG_nnn'CTG Cuts at: 2531 3510 5082 2531 Size: 979 1572 3216 Fragments arranged by size: 3216 1572 979 ApaI G_GGCC'C Cuts at: 3048 3048 Size: 5767 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 3092 1870 Size: 73 1149 4545 Fragments arranged by size: 4545 1149 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2511 2544 2511 Size: 33 5734 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3088 4543 618 Size: 325 217 627 1301 1455 1842 Fragments arranged by size: 1842 1455 1301 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 3048 3086 1391 Size: 678 979 38 4072 Fragments arranged by size: 4072 979 678 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2751 928 Size: 971 852 3944 Fragments arranged by size: 3944 971 852 BbvCI CC'TCA_GC Cuts at: 2210 2210 Size: 5767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4378 136 Size: 107 122 71 631 605 2706 1525 Fragments arranged by size: 2706 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2842 5762 2842 Size: 2920 2847 BlpI GC'TnA_GC Cuts at: 2245 3184 2245 Size: 939 4828 Bme1580I G_kGCm'C Cuts at: 1790 3048 3828 4984 1790 Size: 1258 780 1156 2573 Fragments arranged by size: 2573 1258 1156 780 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 4456 442 Size: 416 53 83 399 24 2081 958 1753 Fragments arranged by size: 2081 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 2744 2915 4427 1239 Size: 814 691 171 1512 2579 Fragments arranged by size: 2579 1512 814 691 171 Bpu10I CC'TnA_GC Cuts at: 2210 2210 Size: 5767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3892 4757 5055 3892 Size: 865 298 4604 Fragments arranged by size: 4604 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2891 4430 882 Size: 617 1392 1539 2219 Fragments arranged by size: 2219 1539 1392 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3574 3956 4591 275 Size: 53 83 186 936 2041 382 635 1451 Fragments arranged by size: 2041 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4200 4598 4872 5019 5430 4200 Size: 398 274 147 411 4537 Fragments arranged by size: 4537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5542 Fragments arranged by size: 5542 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5767 BseYI C'CCAG_C Cuts at: 2374 4970 5466 2374 Size: 2596 496 2675 Fragments arranged by size: 2675 2596 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2356 1163 Size: 573 345 275 4574 Fragments arranged by size: 4574 573 345 275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3828 3913 4984 3828 Size: 85 1071 4611 Fragments arranged by size: 4611 1071 85 BsmI GAATG_Cn' Cuts at: 3278 3371 3278 Size: 93 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 3048 3086 3828 3913 4984 1391 Size: 399 279 979 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 979 742 399 279 85 38 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 2037 3656 5386 2037 Size: 1619 1730 2418 Fragments arranged by size: 2418 1730 1619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2909 2945 3122 844 Size: 2065 36 177 3489 Fragments arranged by size: 3489 2065 177 36 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2449 3007 4262 4436 65 Size: 1183 1201 558 1255 174 1396 Fragments arranged by size: 1396 1255 1201 1183 558 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4411 4598 1700 Size: 110 2601 187 2869 Fragments arranged by size: 2869 2601 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 3856 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 3827 4839 3827 Size: 1012 4755 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 2167 4571 2167 Size: 2404 3363 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstXI CCAn_nnnn'nTGG Cuts at: 2374 2374 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2618 2628 2842 3097 3464 3849 3866 5318 Size: 646 10 214 255 367 385 17 1452 Cuts at: 5318 5404 5416 5762 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 646 385 367 346 255 214 86 17 12 10 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5215 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 3283 4077 4097 3283 Size: 794 20 4953 Fragments arranged by size: 4953 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2251 2286 529 Size: 35 1687 35 4010 Fragments arranged by size: 4010 1687 35 35 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 3214 4103 Size: 54 1425 50 275 18 534 850 889 Cuts at: 4103 5732 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 850 534 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2375 2442 3697 1353 Size: 1022 67 1255 3423 Fragments arranged by size: 3423 1255 1022 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2655 2744 2915 3830 4427 5214 Size: 393 421 602 89 171 915 597 787 Cuts at: 5214 5482 1239 Size: 268 1524 Fragments arranged by size: 1524 915 787 602 597 421 393 268 171 89 EcoO109I rG'GnC_Cy Cuts at: 3044 3045 3044 Size: 1 5766 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 EcoRV GAT'ATC Cuts at: 2508 2508 Size: 5767 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2471 2471 Size: 5767 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 4914 1678 Size: 3236 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 4408 4440 4482 Size: 32 135 32 656 32 2148 32 42 Cuts at: 4482 4514 1373 Size: 32 2626 Fragments arranged by size: 2626 2148 656 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4881 5065 1305 Size: 3576 184 2007 Fragments arranged by size: 3576 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 10 Size: 54 1425 343 534 3411 Fragments arranged by size: 3411 1425 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3725 4084 4243 518 Size: 648 2559 359 159 2042 Fragments arranged by size: 2559 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2943 3183 3860 5008 5253 1606 Size: 450 887 240 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 450 245 240 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5215 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 2683 3000 3125 4670 1132 Size: 97 1454 317 125 1545 2229 Fragments arranged by size: 2229 1545 1454 317 125 97 PasI CC'CwG_GG Cuts at: 1348 3040 1348 Size: 1692 4075 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 2735 1108 Size: 1627 4140 PfoI T'CCnGG_A Cuts at: 2760 2760 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2757 2789 3836 3868 5349 5381 2757 Size: 32 1047 32 1481 32 3143 Fragments arranged by size: 3143 1481 1047 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PspOMI G'GGCC_C Cuts at: 3044 3044 Size: 5767 PstI C_TGCA'G Cuts at: 830 2870 2942 3119 830 Size: 2040 72 177 3478 Fragments arranged by size: 3478 2040 177 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 PvuII CAG'CTG Cuts at: 2056 2943 2056 Size: 887 4880 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2866 2938 3115 4250 4931 5122 826 Size: 219 1821 72 177 1135 681 191 1471 Fragments arranged by size: 1821 1471 1135 681 219 191 177 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 3094 1872 Size: 1222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3871 4772 5034 820 Size: 197 926 1928 901 262 1553 Fragments arranged by size: 1928 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 3206 513 Size: 552 2141 3074 Fragments arranged by size: 3074 2141 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2247 2943 3794 3811 3964 4149 4594 Size: 1255 667 696 851 17 153 185 445 Cuts at: 4594 325 Size: 1498 Fragments arranged by size: 1498 1255 851 696 667 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 4013 5725 Size: 274 80 33 51 1443 30 2006 1712 Cuts at: 5725 96 Size: 138 Fragments arranged by size: 2006 1712 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5727 5759 5727 Size: 32 5735 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 3092 1870 Size: 1222 4545 XmnI GAAnn'nnTTC Cuts at: 2033 2163 2420 3896 2033 Size: 130 257 1476 3904 Fragments arranged by size: 3904 1476 257 130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI