Restriction map for
pFN21AE2273
(Circular) (Six-base) MAPSORT of: pFN21AE2273.seq Check: 9008 from: 1 to: 6382
pFN21AE2273 6382 bp
With 182 enzymes: SgfI *
March 28, 2012 12:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2595 2602 2719 2892 3725 1937
Size: 658 7 117 173 833 4594
Fragments arranged by size:
4594 833 658 173 117 7
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 4509 4882 1563
Size: 2946 373 3063
Fragments arranged by size:
3063 2946 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2679 4445 5829 6097 1632
Size: 1047 1766 1384 268 1917
Fragments arranged by size:
1917 1766 1384 1047 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 3024 5281 6258 1203
Size: 1821 2257 977 1327
Fragments arranged by size:
2257 1821 1327 977
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6382
AlwNI CAG_nnn'CTG
Cuts at: 2133 4125 5697 2133
Size: 1992 1572 2818
Fragments arranged by size:
2818 1992 1572
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 2174 2241 2428 3147 3582 3691 3917 4069
Size: 681 67 187 719 435 109 226 152
Cuts at: 4069 6178 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 719 681 435 226 187 152
109 67
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 3707 1870
Size: 73 1764 4545
Fragments arranged by size:
4545 1764 73
BamHI G'GATC_C
Cuts at: 2088 3712 4079 2088
Size: 1624 367 4391
Fragments arranged by size:
4391 1624 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3077 3703 5158 618
Size: 325 217 627 1290 626 1455 1842
Fragments arranged by size:
1842 1455 1290 627 626 325 217
BanII G_rGCy'C
Cuts at: 1391 2049 3701 1391
Size: 658 1652 4072
Fragments arranged by size:
4072 1652 658
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2378 3222 3410 928
Size: 971 479 844 188 3900
Fragments arranged by size:
3900 971 844 479 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4573 4607 1903
Size: 34 2636 34 3678
Fragments arranged by size:
3678 2636 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 5484 4274
Size: 1210 5172
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6382
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3086 3320 4993
Size: 107 122 71 631 605 1414 234 1673
Cuts at: 4993 136
Size: 1525
Fragments arranged by size:
1673 1525 1414 631 605 234 122 107
71
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 3799 3799
Size: 6382
Bme1580I G_kGCm'C
Cuts at: 1790 4443 5599 1790
Size: 2653 1156 2573
Fragments arranged by size:
2653 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6382
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2244 3411 4113
Size: 416 53 83 399 24 827 1167 702
Cuts at: 4113 5071 442
Size: 958 1753
Fragments arranged by size:
1753 1167 958 827 702 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2748 3474 2748
Size: 726 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2797 3499 3621 5042 1239
Size: 1558 702 122 1421 2579
Fragments arranged by size:
2579 1558 1421 702 122
Bpu10I CC'TnA_GC
Cuts at: 3609 3609
Size: 6382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4507 5372 5670 4507
Size: 865 298 5219
Fragments arranged by size:
5219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5045 882
Size: 617 3546 2219
Fragments arranged by size:
3546 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3025 4173 493
Size: 711 1821 1148 2702
Fragments arranged by size:
2702 1821 1148 711
BsaBI GATnn'nnATC
Cuts at: 6376 6376
Size: 6382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 3596 4189 4571
Size: 53 83 186 936 569 1494 593 382
Cuts at: 4571 5206 275
Size: 635 1451
Fragments arranged by size:
1494 1451 936 635 593 569 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2679 2887 4815 5213 5487 5634 6045 2679
Size: 208 1928 398 274 147 411 3016
Fragments arranged by size:
3016 1928 411 398 274 208 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 3338 3368 3514
Size: 30 165 30 633 30 1272 30 146
Cuts at: 3514 3544 1178
Size: 30 4016
Fragments arranged by size:
4016 1272 633 165 146 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2271 3413 1186
Size: 1085 1142 4155
Fragments arranged by size:
4155 1142 1085
BseYI C'CCAG_C
Cuts at: 2081 2340 2412 3000 3284 3325 5585 6081 2081
Size: 259 72 588 284 41 2260 496 2382
Fragments arranged by size:
2382 2260 588 496 284 259 72 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5809
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3832 4593 5621 1540
Size: 275 186 1831 761 1028 2301
Fragments arranged by size:
2301 1831 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4443 4528 5599 4443
Size: 85 1071 5226
Fragments arranged by size:
5226 1071 85
BsmI GAATG_Cn'
Cuts at: 3893 3986 3893
Size: 93 6289
BsmBI CGTCTCn'nnnn_
Cuts at: 3587 3597 3587
Size: 10 6372
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 3701 4443 4528 5599 1391
Size: 399 259 1652 742 85 1071 2174
Fragments arranged by size:
2174 1652 1071 742 399 259 85
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 4271 6001 4271
Size: 1730 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3737 844
Size: 2893 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4269 1394
Size: 429 2446 3507
Fragments arranged by size:
3507 2446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4877 5051 65
Size: 1183 3629 174 1396
Fragments arranged by size:
3629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 5026 5213 1700
Size: 110 298 2918 187 2869
Fragments arranged by size:
2918 2869 298 187 110
BsrGI T'GTAC_A
Cuts at: 96 2052 96
Size: 1956 4426
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 4442 5454 4442
Size: 1012 5370
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6382
BstBI TT'CG_AA
Cuts at: 2633 5186 2633
Size: 2553 3829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6382
BstYI r'GATC_y
Cuts at: 1972 2088 2130 2741 3712 4079 4464 4481 5933
Size: 116 42 611 971 367 385 17 1452
Cuts at: 5933 6019 6031 6377 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 971 611 385 367 346 116
86 42 17 12
BstZ17I GTA'TAC
Cuts at: 2596 2603 2596
Size: 7 6375
Bsu36I CC'TnA_GG
Cuts at: 3309 3309
Size: 6382
BtgI C'CryG_G
Cuts at: 513 1065 3099 513
Size: 552 2034 3796
Fragments arranged by size:
3796 2034 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2821 3087 487
Size: 683 56 1595 266 3782
Fragments arranged by size:
3782 1595 683 266 56
BtsI GCAGTG_nn'
Cuts at: 3467 3898 4692 4712 3467
Size: 431 794 20 5137
Fragments arranged by size:
5137 794 431 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6347
DraI TTT'AAA
Cuts at: 3686 3686
Size: 6382
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2123 5389 809
Size: 507 807 3266 1802
Fragments arranged by size:
3266 1802 807 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3087 3829 4718
Size: 54 1425 50 275 18 1257 742 889
Cuts at: 4718 6347 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1257 889 742 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3829 1537
Size: 275 2017 4090
Fragments arranged by size:
4090 2017 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4312 1353
Size: 2959 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4969 5343 5489 387
Size: 1064 138 3380 374 146 1280
Fragments arranged by size:
3380 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2679 2797 3499 3621 4445 5042 5829
Size: 393 1047 118 702 122 824 597 787
Cuts at: 5829 6097 1239
Size: 268 1524
Fragments arranged by size:
1524 1047 824 787 702 597 393 268
122 118
EcoO109I rG'GnC_Cy
Cuts at: 2542 3239 3322 3346 3389 2542
Size: 697 83 24 43 5535
Fragments arranged by size:
5535 697 83 43 24
EcoRI G'AATT_C
Cuts at: 3147 3691 4069 3147
Size: 544 378 5460
Fragments arranged by size:
5460 544 378
EcoRV GAT'ATC
Cuts at: 2496 2496
Size: 6382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2885 3158 2728
Size: 157 273 5952
Fragments arranged by size:
5952 273 157
FspI TGC'GCA
Cuts at: 4888 4888
Size: 6382
HaeII r_GCGC'y
Cuts at: 1678 5529 1678
Size: 3851 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3292 3324 3338 3370 3514
Size: 32 135 32 1720 32 14 32 144
Cuts at: 3514 3546 5023 5055 5097 5129 1373
Size: 32 1477 32 42 32 2626
Fragments arranged by size:
2626 1720 1477 144 135 42 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 3972 669
Size: 988 281 1788 246 3079
Fragments arranged by size:
3079 1788 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6382
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 3981 3981
Size: 6382
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2255 5496 5680 1305
Size: 950 3241 184 2007
Fragments arranged by size:
3241 2007 950 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3089 10
Size: 54 1425 343 1257 3303
Fragments arranged by size:
3303 1425 1257 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4340 4699 4858 518
Size: 648 3174 359 159 2042
Fragments arranged by size:
3174 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 2762 3798 4475 5623 5868 1606
Size: 738 418 1036 677 1148 245 2120
Fragments arranged by size:
2120 1148 1036 738 677 418 245
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 6382
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5830
NdeI CA'TA_TG
Cuts at: 387 2468 387
Size: 2081 4301
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 6382
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 3740 5285 1132
Size: 97 2511 1545 2229
Fragments arranged by size:
2511 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 5281 5281
Size: 6382
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2098 2469 2855 1108
Size: 990 371 386 4635
Fragments arranged by size:
4635 990 386 371
PfoI T'CCnGG_A
Cuts at: 2813 2854 3446 2813
Size: 41 592 5749
Fragments arranged by size:
5749 592 41
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 5964 5996 4451
Size: 32 1481 32 4837
Fragments arranged by size:
4837 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2542 3239 3346 2542
Size: 697 107 5578
Fragments arranged by size:
5578 697 107
PshAI GACnn'nnGTC
Cuts at: 1613 2101 1613
Size: 488 5894
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3175 3207 3175
Size: 32 6350
PstI C_TGCA'G
Cuts at: 830 3414 3734 830
Size: 2584 320 3478
Fragments arranged by size:
3478 2584 320
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
PvuII CAG'CTG
Cuts at: 2344 2762 2344
Size: 418 5964
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SanDI GG'GwC_CC
Cuts at: 2542 3346 2542
Size: 804 5578
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SfcI C'TryA_G
Cuts at: 826 1045 3410 3573 3730 4865 5546 5737 826
Size: 219 2365 163 157 1135 681 191 1471
Fragments arranged by size:
2365 1471 1135 681 219 191 163 157
SfiI GGCCn_nnn'nGGCC
Cuts at: 3320 3320
Size: 6382
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 3709 1872
Size: 1837 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4486 5387 5649 820
Size: 197 926 2543 901 262 1553
Fragments arranged by size:
2543 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 4306 5
Size: 47 4254 2081
Fragments arranged by size:
4254 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6382
StyI C'CwwG_G
Cuts at: 513 1065 2527 3821 513
Size: 552 1462 1294 3074
Fragments arranged by size:
3074 1462 1294 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3014 3456 4409 4426 4579 4764 5209
Size: 1255 1434 442 953 17 153 185 445
Cuts at: 5209 325
Size: 1498
Fragments arranged by size:
1498 1434 1255 953 445 442 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 4628 6340
Size: 274 80 33 51 1443 75 2576 1712
Cuts at: 6340 96
Size: 138
Fragments arranged by size:
2576 1712 1443 274 138 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6342 6374 6342
Size: 32 6350
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6382
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 3707 1870
Size: 1837 4545
XmnI GAAnn'nnTTC
Cuts at: 2708 3582 4511 2708
Size: 874 929 4579
Fragments arranged by size:
4579 929 874
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI DraIII EcoICRI EcoNI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI RsrII SacI SacII SapI SexAI SfoI SrfI
SwaI XcmI