(Linear) (Six-base) MAPSORT of: KIEE2273.seq Check: 8656 from: 1 to: 1677 KIEE2273.orf With 182 enzymes: SgfI * March 22, 2012 16:01 .. AccI GT'mk_AC Cuts at: 0 590 597 714 887 1677 Size: 590 7 117 173 790 Fragments arranged by size: 790 590 173 117 7 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 674 1677 Size: 674 1003 AflIII A'CryG_T Cuts at: 0 1019 1677 Size: 1019 658 AlwNI CAG_nnn'CTG Cuts at: 0 128 1677 Size: 128 1549 ApoI r'AATT_y Cuts at: 0 169 236 423 1142 1577 1677 Size: 169 67 187 719 435 100 Fragments arranged by size: 719 435 187 169 100 67 BamHI G'GATC_C Cuts at: 0 83 1677 Size: 83 1594 BanI G'GyrC_C Cuts at: 0 1072 1677 Size: 1072 605 BanII G_rGCy'C Cuts at: 0 44 1677 Size: 44 1633 BbsI GAAGACnn'nnnn_ Cuts at: 0 373 1217 1405 1677 Size: 373 844 188 272 Fragments arranged by size: 844 373 272 188 BglI GCCn_nnn'nGGC Cuts at: 0 1081 1315 1677 Size: 1081 234 362 Fragments arranged by size: 1081 362 234 BmrI ACTGGGnnnn_n' Cuts at: 0 239 1406 1677 Size: 239 1167 271 Fragments arranged by size: 1167 271 239 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 743 1469 1677 Size: 743 726 208 Fragments arranged by size: 743 726 208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 792 1494 1616 1677 Size: 792 702 122 61 Fragments arranged by size: 792 702 122 61 Bpu10I CC'TnA_GC Cuts at: 0 1604 1677 Size: 1604 73 BsaAI yAC'GTr Cuts at: 0 1020 1677 Size: 1020 657 BsaHI Gr'CG_yC Cuts at: 0 97 1591 1677 Size: 97 1494 86 Fragments arranged by size: 1494 97 86 BsaWI w'CCGG_w Cuts at: 0 674 882 1677 Size: 674 208 795 Fragments arranged by size: 795 674 208 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 31 61 1333 1363 1509 1539 1677 Size: 31 30 1272 30 146 30 138 Fragments arranged by size: 1272 146 138 31 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 266 1408 1677 Size: 266 1142 269 Fragments arranged by size: 1142 269 266 BseYI C'CCAG_C Cuts at: 0 76 335 407 995 1279 1320 1677 Size: 76 259 72 588 284 41 357 Fragments arranged by size: 588 357 284 259 76 72 41 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1582 1592 1677 Size: 1582 10 85 Fragments arranged by size: 1582 85 10 Bsp1286I G_dGCh'C Cuts at: 0 44 1677 Size: 44 1633 BsrFI r'CCGG_y Cuts at: 0 103 1677 Size: 103 1574 BsrGI T'GTAC_A Cuts at: 0 47 1677 Size: 47 1630 BstBI TT'CG_AA Cuts at: 0 628 1677 Size: 628 1049 BstYI r'GATC_y Cuts at: 0 83 125 736 1677 Size: 83 42 611 941 Fragments arranged by size: 941 611 83 42 BstZ17I GTA'TAC Cuts at: 0 591 598 1677 Size: 591 7 1079 Fragments arranged by size: 1079 591 7 Bsu36I CC'TnA_GG Cuts at: 0 1304 1677 Size: 1304 373 BtgI C'CryG_G Cuts at: 0 1094 1677 Size: 1094 583 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 816 1082 1677 Size: 816 266 595 Fragments arranged by size: 816 595 266 BtsI GCAGTG_nn' Cuts at: 0 1462 1677 Size: 1462 215 DrdI GACnn_nn'nnGTC Cuts at: 0 118 1677 Size: 118 1559 EaeI y'GGCC_r Cuts at: 0 1082 1677 Size: 1082 595 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 674 792 1494 1616 1677 Size: 674 118 702 122 61 Fragments arranged by size: 702 674 122 118 61 EcoO109I rG'GnC_Cy Cuts at: 0 537 1234 1317 1341 1384 1677 Size: 537 697 83 24 43 293 Fragments arranged by size: 697 537 293 83 43 24 EcoRI G'AATT_C Cuts at: 0 1142 1677 Size: 1142 535 EcoRV GAT'ATC Cuts at: 0 491 1677 Size: 491 1186 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 723 880 1153 1677 Size: 723 157 273 524 Fragments arranged by size: 723 524 273 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1287 1319 1333 1365 1509 1541 1677 Size: 1287 32 14 32 144 32 136 Fragments arranged by size: 1287 144 136 32 32 32 14 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 250 1677 Size: 250 1427 MscI TGG'CCA Cuts at: 0 1084 1677 Size: 1084 593 MspA1I CmG'CkG Cuts at: 0 339 757 1677 Size: 339 418 920 Fragments arranged by size: 920 418 339 NaeI GCC'GGC Cuts at: 0 105 1677 Size: 105 1572 NdeI CA'TA_TG Cuts at: 0 463 1677 Size: 463 1214 NgoMIV G'CCGG_C Cuts at: 0 103 1677 Size: 103 1574 PflMI CCAn_nnn'nTGG Cuts at: 0 93 464 850 1677 Size: 93 371 386 827 Fragments arranged by size: 827 386 371 93 PfoI T'CCnGG_A Cuts at: 0 808 849 1441 1677 Size: 808 41 592 236 Fragments arranged by size: 808 592 236 41 PpuMI rG'GwC_Cy Cuts at: 0 537 1234 1341 1677 Size: 537 697 107 336 Fragments arranged by size: 697 537 336 107 PshAI GACnn'nnGTC Cuts at: 0 96 1677 Size: 96 1581 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1170 1202 1677 Size: 1170 32 475 Fragments arranged by size: 1170 475 32 PstI C_TGCA'G Cuts at: 0 1409 1677 Size: 1409 268 PvuII CAG'CTG Cuts at: 0 339 757 1677 Size: 339 418 920 Fragments arranged by size: 920 418 339 SanDI GG'GwC_CC Cuts at: 0 537 1341 1677 Size: 537 804 336 Fragments arranged by size: 804 537 336 SfcI C'TryA_G Cuts at: 0 1405 1568 1677 Size: 1405 163 109 Fragments arranged by size: 1405 163 109 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1315 1677 Size: 1315 362 StyI C'CwwG_G Cuts at: 0 522 1677 Size: 522 1155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1009 1451 1677 Size: 1009 442 226 Fragments arranged by size: 1009 442 226 TatI w'GTAC_w Cuts at: 0 47 1677 Size: 47 1630 XmnI GAAnn'nnTTC Cuts at: 0 703 1577 1677 Size: 703 874 100 Fragments arranged by size: 874 703 100 Enzymes that do cut: AccI AcuI AflIII AlwNI ApoI BamHI BanI BanII BbsI BglI BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsmBI Bsp1286I BsrFI BsrGI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DrdI EaeI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I MmeI MscI MspA1I NaeI NdeI NgoMIV PflMI PfoI PpuMI PshAI PsrI PstI PvuII SanDI SfcI SfiI StyI TaqII TatI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaI BsaBI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstEII BstXI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MslI NarI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI