################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 63 GCGG CCGC(-3/-1) AvaII 175 GGACC G^G[AT]CC BfaI 188 CTAG C^TAG BsaJI 317 CCCAGG C^C[ACGT][ACGT]GG BseRI 27 CTCCTC GAGGAG(10/8) BsmAI 49 GTCTC GTCTC(1/5) BsmBI 48 CGTCTC CGTCTC(1/5) BssKI 318 CCAGG ^CC[ACGT]GG BstNI 318 CCAGG CC^[AT]GG BstYI 288 AGATCC [GA]^GATC[CT] Cfr13I 175 GGACC G^G[ACGT]CC DraII 174 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 16 CTCTTC CTCTTC(1/4) EcoO109I 174 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 318 CCAGG ^CC[AT]GG HaeIII 321 GGCC GG^CC HinfI 3 GATTC G^A[ACGT]TC Ksp632I 16 CTCTTC CTCTTC(1/4) MaeI 188 CTAG C^TAG MaeIII 79 GTTAC ^GT[ACGT]AC MflI 288 AGATCC [GA]^GATC[CT] MvaI 318 CCAGG CC^[AT]GG MwoI 222 GCTTTTTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 258 CATG CATG^ PpuMI 174 AGGACCT [GA]G^G[AT]CC[CT] SapI 15 GCTCTTC GCTCTTC(1/4) Sau96I 175 GGACC G^G[ACGT]CC ScrFI 318 CCAGG CC^[ACGT]GG SfaNI 184 GATGC GCATC(5/9) SmlI 232 CTCAAG C^T[CT][GA]AG TaqI 308 TCGA T^CGA TfiI 3 GATTC G^A[AT]TC TspRI 86 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 308 TCGA T^CGA XhoII 288 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcsI 280 AAATTT [GA]^AATT[CT] 295 AAATTT AlwI 109 GATCC GGATC(4/5) 289 GATCC ApoI 280 AAATTT [GA]^AATT[CT] 295 AAATTT BpmI 170 CTGGAG CTGGAG(16/14) 211 CTGGAG DdeI 51 CTCAG C^T[ACGT]AG 155 CTAAG DpnII 109 GATC ^GATC 289 GATC MboI 109 GATC ^GATC 289 GATC MseI 95 TTAA T^TAA 227 TTAA NdeII 109 GATC ^GATC 289 GATC Sau3AI 109 GATC ^GATC 289 GATC Tru9I 95 TTAA T^TAA 227 TTAA Tsp509I 281 AATT ^AATT 296 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AluI MboII MnlI