################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AlwI AocI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DrdI DsaI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KspI MaeI MaeIII MamI MboI MfeI MflI MluI MroI MseI MunI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI Sau3AI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 612 GGTACC G^GTACC AflIII 205 ACGTGT A^C[GA][CT]GT Asp718I 612 GGTACC G^GTACC AvaII 727 GGTCC G^G[AT]CC BalI 845 TGGCCA TGG^CCA BanII 787 GAGCTC G[GA]GC[CT]^C BsiEI 178 CGGCCG CG[GA][CT]^CG BsmFI 116 GGGAC GGGAC(10/14) BstF5I 851 GGATG GGATG(2/0) EagI 178 CGGCCG C^GGCCG EarI 555 CTCTTC CTCTTC(1/4) EclXI 178 CGGCCG C^GGCCG Eco52I 178 CGGCCG C^GGCCG EcoT14I 736 CCAAGG C^C[AT][AT]GG FokI 851 GGATG GGATG(9/13) KpnI 612 GGTACC GGTAC^C Ksp632I 555 CTCTTC CTCTTC(1/4) MluNI 845 TGGCCA TGG^CCA MscI 845 TGGCCA TGG^CCA NlaIII 565 CATG CATG^ PaeR7I 706 CTCGAG C^TCGAG SacI 787 GAGCTC GAGCT^C SapI 554 GCTCTTC GCTCTTC(1/4) SexAI 399 ACCTGGT A^CC[AT]GGT SfiI 702 GGCCCTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 706 CTCGAG C^T[CT][GA]AG StuI 21 AGGCCT AGG^CCT StyI 736 CCAAGG C^C[AT][AT]GG XhoI 706 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 79 GTAC GT^AC 613 GTAC Alw44I 576 GTGCAC G^TGCAC 895 GTGCAC Aor51HI 257 AGCGCT AGC^GCT 881 AGCGCT ApaLI 576 GTGCAC G^TGCAC 895 GTGCAC BbvCI 600 CCTCAGC CCTCAGC(-5/-2) 622 GCTGAGG BsmAI 124 GAGAC GTCTC(1/5) 191 GAGAC BsrBI 133 GAGCGG CCGCTC(-3/-3) 865 GAGCGG BsrI 589 ACTGG ACTGG(1/-1) 150 CCAGT DraII 701 AGGCCCT [GA]G^G[ACGT]CC[CT] 710 AGGCCCC EaeI 178 CGGCCG [CT]^GGCC[GA] 845 TGGCCA Eco47III 257 AGCGCT AGC^GCT 881 AGCGCT EcoO109I 701 AGGCCCT [GA]G^G[ACGT]CC[CT] 710 AGGCCCC MaeII 205 ACGT A^CGT 275 ACGT MboII 356 TCTTC GAAGA(8/7) 556 TCTTC MslI 165 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 325 CACAACCGTG NaeI 72 GCCGGC GCC^GGC 406 GCCGGC NgoMIV 72 GCCGGC G^CCGGC 406 GCCGGC PvuII 582 CAGCTG CAG^CTG 760 CAGCTG RsaI 79 GTAC GT^AC 613 GTAC SfaNI 852 GATGC GCATC(5/9) 388 GCATC SmaI 420 CCCGGG CCC^GGG 856 CCCGGG TaqI 647 TCGA T^CGA 707 TCGA TthHB8I 647 TCGA T^CGA 707 TCGA XmaCI 420 CCCGGG C^CCGGG 856 CCCGGG XmaI 420 CCCGGG C^CCGGG 856 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI AvaI BanI BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraIII EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PstI Sau96I ScrFI SfcI TseI TspRI