(Circular) (Six-base) MAPSORT of: pFN21AB9520.seq Check: 7549 from: 1 to: 6040 pFN21AB9520 6040 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4103 5487 5755 1632 Size: 2471 1384 268 1917 Fragments arranged by size: 2471 1917 1384 268 AfeI AGC'GCT Cuts at: 3044 3044 Size: 6040 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3783 5355 3783 Size: 1572 4468 ApaI G_GGCC'C Cuts at: 2437 3121 2437 Size: 684 5356 ApaLI G'TGCA_C Cuts at: 2356 4097 5253 2356 Size: 1741 1156 3143 Fragments arranged by size: 3143 1741 1156 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2191 2415 2555 2712 2729 2741 2876 Size: 73 248 224 140 157 17 12 135 Cuts at: 2876 2935 3057 3365 1870 Size: 59 122 308 4545 Fragments arranged by size: 4545 308 248 224 157 140 135 122 73 59 17 12 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2255 3361 4816 618 Size: 325 217 627 468 1106 1455 1842 Fragments arranged by size: 1842 1455 1106 627 468 325 217 BanII G_rGCy'C Cuts at: 1391 2437 2713 2736 2975 3111 3121 3359 1391 Size: 1046 276 23 239 136 10 238 4072 Fragments arranged by size: 4072 1046 276 239 238 136 23 10 BbeI G_GCGC'C Cuts at: 2259 2259 Size: 6040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5069 BbvCI CC'TCA_GC Cuts at: 2639 2660 2856 2639 Size: 21 196 5823 Fragments arranged by size: 5823 196 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 3138 1548 Size: 1590 4450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 2183 2867 Size: 107 122 71 631 605 354 157 684 Cuts at: 2867 3218 4651 136 Size: 351 1433 1525 Fragments arranged by size: 1525 1433 684 631 605 354 351 157 122 107 71 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2360 2437 2802 2945 3121 4101 5257 1790 Size: 570 77 365 143 176 980 1156 2573 Fragments arranged by size: 2573 1156 980 570 365 176 143 77 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 4729 442 Size: 416 53 83 399 24 2354 958 1753 Fragments arranged by size: 2354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2639 2902 3023 3344 4700 1239 Size: 1400 263 121 321 1356 2579 Fragments arranged by size: 2579 1400 1356 321 263 121 Bpu10I CC'TnA_GC Cuts at: 2519 2639 2660 2856 2519 Size: 120 21 196 5703 Fragments arranged by size: 5703 196 120 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2948 4165 5030 5328 2948 Size: 1217 865 298 3660 Fragments arranged by size: 3660 1217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2428 4703 882 Size: 617 929 2275 2219 Fragments arranged by size: 2275 2219 929 617 BsaAI yAC'GTr Cuts at: 493 1204 2287 3831 493 Size: 711 1083 1544 2702 Fragments arranged by size: 2702 1544 1083 711 BsaBI GATnn'nnATC Cuts at: 3140 6034 3140 Size: 2894 3146 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 2987 3847 4229 Size: 53 83 186 936 723 731 860 382 Cuts at: 4229 4864 275 Size: 635 1451 Fragments arranged by size: 1451 936 860 731 723 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2549 3176 3206 1178 Size: 30 165 30 1116 30 627 30 4012 Fragments arranged by size: 4012 1116 627 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2353 2923 1186 Size: 1167 570 4303 Fragments arranged by size: 4303 1167 570 BseYI C'CCAG_C Cuts at: 2152 2436 2523 2748 2778 3039 3114 5243 5739 Size: 284 87 225 30 261 75 2129 496 Cuts at: 5739 2152 Size: 2453 Fragments arranged by size: 2453 2129 496 284 261 225 87 75 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2804 1163 Size: 573 1068 4399 Fragments arranged by size: 4399 1068 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2314 2398 2847 3490 4251 5279 Size: 275 186 313 84 449 643 761 1028 Cuts at: 5279 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 643 449 313 275 186 84 BsiHKAI G_wGCw'C Cuts at: 2360 2385 3330 4101 4186 5257 2360 Size: 25 945 771 85 1071 3143 Fragments arranged by size: 3143 1071 945 771 85 25 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2360 2385 2437 2713 2736 2802 2945 Size: 399 570 25 52 276 23 66 143 Cuts at: 2945 2975 3111 3121 3330 3359 4101 4186 5257 Size: 30 136 10 209 29 742 85 1071 Cuts at: 5257 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 570 399 276 209 143 136 85 66 52 30 29 25 23 10 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 3395 844 Size: 1514 1037 3489 Fragments arranged by size: 3489 1514 1037 BsrBI CCG'CTC Cuts at: 1394 1823 2420 3927 1394 Size: 429 597 1507 3507 Fragments arranged by size: 3507 1507 597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2723 4684 4871 1700 Size: 110 913 1961 187 2869 Fragments arranged by size: 2869 1961 913 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 2011 2013 2081 1925 Size: 86 2 68 5884 Fragments arranged by size: 5884 86 68 2 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 2410 1184 Size: 1226 4814 BstXI CCAn_nnnn'nTGG Cuts at: 2152 3082 2152 Size: 930 5110 BstYI r'GATC_y Cuts at: 1972 3370 3737 4122 4139 5591 5677 5689 6035 Size: 1398 367 385 17 1452 86 12 346 Cuts at: 6035 1972 Size: 1977 Fragments arranged by size: 1977 1452 1398 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2106 2145 2901 3157 513 Size: 552 939 102 39 756 256 3396 Fragments arranged by size: 3396 939 756 552 256 102 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2466 2563 3163 3328 3556 4350 4370 2466 Size: 97 600 165 228 794 20 4136 Fragments arranged by size: 4136 794 600 228 165 97 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2249 5047 809 Size: 507 933 2798 1802 Fragments arranged by size: 2798 1802 933 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2142 2184 Size: 54 1425 50 275 18 285 27 42 Cuts at: 2184 2311 2844 3072 3084 3160 3487 4376 6005 Size: 127 533 228 12 76 327 889 1629 Cuts at: 6005 8 Size: 43 Fragments arranged by size: 1629 1425 889 533 327 285 275 228 127 76 54 50 43 42 27 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2311 2844 3487 1537 Size: 275 499 533 643 4090 Fragments arranged by size: 4090 643 533 499 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3970 1353 Size: 2617 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3251 4627 5001 5147 387 Size: 1064 138 1662 1376 374 146 1280 Fragments arranged by size: 1662 1376 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2639 2902 3023 3344 4103 4700 5487 Size: 393 1007 263 121 321 759 597 787 Cuts at: 5487 5755 1239 Size: 268 1524 Fragments arranged by size: 1524 1007 787 759 597 393 321 268 263 121 EcoO109I rG'GnC_Cy Cuts at: 2317 3118 2317 Size: 801 5239 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 2051 2132 2259 2856 3046 5187 1678 Size: 373 81 127 597 190 2141 2531 Fragments arranged by size: 2531 2141 597 373 190 127 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2499 2519 2531 2551 4681 Size: 32 135 32 927 20 12 20 2130 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2130 927 135 42 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3226 3384 3630 669 Size: 988 281 1288 158 246 3079 Fragments arranged by size: 3079 1288 988 281 246 158 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KasI G'GCGC_C Cuts at: 2255 2255 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5154 5338 1305 Size: 960 2889 184 2007 Fragments arranged by size: 2889 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 3074 3162 10 Size: 54 1425 343 312 930 88 2888 Fragments arranged by size: 2888 1425 930 343 312 88 54 MslI CAynn'nnrTG Cuts at: 518 1166 2568 2826 3017 3998 4357 4516 518 Size: 648 1402 258 191 981 359 159 2042 Fragments arranged by size: 2042 1402 981 648 359 258 191 159 MspA1I CmG'CkG Cuts at: 1606 2032 2108 2301 2818 3456 4133 5281 5526 Size: 426 76 193 517 638 677 1148 245 Cuts at: 5526 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 638 517 426 245 193 76 NaeI GCC'GGC Cuts at: 2725 2725 Size: 6040 NarI GG'CG_CC Cuts at: 2256 2256 Size: 6040 NcoI C'CATG_G Cuts at: 513 1065 2004 3157 513 Size: 552 939 1153 3396 Fragments arranged by size: 3396 1153 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NgoMIV G'CCGG_C Cuts at: 2723 2723 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3398 4943 1132 Size: 97 2169 1545 2229 Fragments arranged by size: 2229 2169 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2771 1108 Size: 1663 4377 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PspOMI G'GGCC_C Cuts at: 2433 3117 2433 Size: 684 5356 PspXI vC'TCGA_Gb Cuts at: 2191 2935 2191 Size: 744 5296 PstI C_TGCA'G Cuts at: 830 3392 830 Size: 2562 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 SacII CC_GC'GG Cuts at: 2109 2109 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 3388 4523 5204 5395 826 Size: 219 2343 1135 681 191 1471 Fragments arranged by size: 2343 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2257 2257 Size: 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2191 2712 2935 2963 4144 5045 Size: 197 926 248 521 223 28 1181 901 Cuts at: 5045 5307 820 Size: 262 1553 Fragments arranged by size: 1553 1181 926 901 521 262 248 223 197 28 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2004 3157 3479 513 Size: 552 939 1153 322 3074 Fragments arranged by size: 3074 1153 939 552 322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 2398 2473 2825 2926 3053 4067 Size: 1255 639 179 75 352 101 127 1014 Cuts at: 4067 4084 4237 4422 4867 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1014 639 445 352 185 179 153 127 101 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4286 5998 96 Size: 274 80 33 51 1443 2309 1712 138 Fragments arranged by size: 2309 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 2191 2712 2935 1943 Size: 248 521 223 5048 Fragments arranged by size: 5048 521 248 223 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 2213 4169 2213 Size: 1956 4084 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI