(Linear) (Six-base) MAPSORT of: KIEE5634.seq Check: 8520 from: 1 to: 1752 KIEE5634.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AccI GT'mk_AC Cuts at: 0 112 143 1752 Size: 112 31 1609 Fragments arranged by size: 1609 112 31 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 935 1752 Size: 935 817 AflII C'TTAA_G Cuts at: 0 961 1752 Size: 961 791 AflIII A'CryG_T Cuts at: 0 77 1752 Size: 77 1675 AhdI GACnn_n'nnGTC Cuts at: 0 78 963 1509 1752 Size: 78 885 546 243 Fragments arranged by size: 885 546 243 78 AlwNI CAG_nnn'CTG Cuts at: 0 1456 1752 Size: 1456 296 ApaLI G'TGCA_C Cuts at: 0 1572 1752 Size: 1572 180 ApoI r'AATT_y Cuts at: 0 586 1014 1752 Size: 586 428 738 Fragments arranged by size: 738 586 428 AvaI C'yCGr_G Cuts at: 0 190 256 1752 Size: 190 66 1496 Fragments arranged by size: 1496 190 66 BamHI G'GATC_C Cuts at: 0 504 1752 Size: 504 1248 BanI G'GyrC_C Cuts at: 0 261 618 1081 1752 Size: 261 357 463 671 Fragments arranged by size: 671 463 357 261 BanII G_rGCy'C Cuts at: 0 1495 1752 Size: 1495 257 BbsI GAAGACnn'nnnn_ Cuts at: 0 41 926 1752 Size: 41 885 826 Fragments arranged by size: 885 826 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1246 1280 1587 1621 1752 Size: 1246 34 307 34 131 Fragments arranged by size: 1246 307 131 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1734 1752 Size: 1734 18 BglI GCCn_nnn'nGGC Cuts at: 0 649 1752 Size: 649 1103 Bme1580I G_kGCm'C Cuts at: 0 467 1043 1576 1752 Size: 467 576 533 176 Fragments arranged by size: 576 533 467 176 BmrI ACTGGGnnnn_n' Cuts at: 0 1563 1714 1752 Size: 1563 151 38 Fragments arranged by size: 1563 151 38 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1512 1752 Size: 1512 240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 368 555 823 1389 1752 Size: 368 187 268 566 363 Fragments arranged by size: 566 368 363 268 187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1688 1752 Size: 1688 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 317 988 1626 1752 Size: 317 671 638 126 Fragments arranged by size: 671 638 317 126 BsaWI w'CCGG_w Cuts at: 0 319 420 1752 Size: 319 101 1332 Fragments arranged by size: 1332 319 101 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 557 1189 1485 1752 Size: 557 632 296 267 Fragments arranged by size: 632 557 296 267 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1253 1752 Size: 1253 499 BsiHKAI G_wGCw'C Cuts at: 0 1473 1576 1752 Size: 1473 103 176 Fragments arranged by size: 1473 176 103 Bsp1286I G_dGCh'C Cuts at: 0 467 1043 1473 1495 1576 1752 Size: 467 576 430 22 81 176 Fragments arranged by size: 576 467 430 176 81 22 BspHI T'CATG_A Cuts at: 0 860 1752 Size: 860 892 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1077 1752 Size: 1077 675 BstAPI GCAn_nnn'nTGC Cuts at: 0 268 1752 Size: 268 1484 BstEII G'GTnAC_C Cuts at: 0 4 1752 Size: 4 1748 BstXI CCAn_nnnn'nTGG Cuts at: 0 1006 1752 Size: 1006 746 BstYI r'GATC_y Cuts at: 0 504 1752 Size: 504 1248 BtgI C'CryG_G Cuts at: 0 1586 1752 Size: 1586 166 BtsI GCAGTG_nn' Cuts at: 0 1603 1752 Size: 1603 149 DraI TTT'AAA Cuts at: 0 873 1671 1752 Size: 873 798 81 Fragments arranged by size: 873 798 81 EaeI y'GGCC_r Cuts at: 0 176 1752 Size: 176 1576 EarI CTCTTCn'nnn_ Cuts at: 0 1531 1752 Size: 1531 221 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 368 555 823 935 1389 1752 Size: 368 187 268 112 454 363 Fragments arranged by size: 454 368 363 268 187 112 EcoNI CCTnn'n_nnAGG Cuts at: 0 308 527 1752 Size: 308 219 1225 Fragments arranged by size: 1225 308 219 EcoO109I rG'GnC_Cy Cuts at: 0 486 789 912 1099 1752 Size: 486 303 123 187 653 Fragments arranged by size: 653 486 303 187 123 EcoRI G'AATT_C Cuts at: 0 1014 1752 Size: 1014 738 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 282 314 355 387 949 981 Size: 64 32 186 32 41 32 562 32 Cuts at: 981 1495 1527 1585 1617 1708 1740 1752 Size: 514 32 58 32 91 32 12 Fragments arranged by size: 562 514 186 91 64 58 41 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 0 113 144 732 1752 Size: 113 31 588 1020 Fragments arranged by size: 1020 588 113 31 HindIII A'AGCT_T Cuts at: 0 358 1752 Size: 358 1394 MluI A'CGCG_T Cuts at: 0 77 1752 Size: 77 1675 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1676 1752 Size: 1676 76 MscI TGG'CCA Cuts at: 0 178 1752 Size: 178 1574 MspA1I CmG'CkG Cuts at: 0 268 430 955 1409 1752 Size: 268 162 525 454 343 Fragments arranged by size: 525 454 343 268 162 PasI CC'CwG_GG Cuts at: 0 460 784 1752 Size: 460 324 968 Fragments arranged by size: 968 460 324 PpuMI rG'GwC_Cy Cuts at: 0 486 789 912 1099 1752 Size: 486 303 123 187 653 Fragments arranged by size: 653 486 303 187 123 PshAI GACnn'nnGTC Cuts at: 0 1347 1752 Size: 1347 405 PsiI TTA'TAA Cuts at: 0 1210 1752 Size: 1210 542 PstI C_TGCA'G Cuts at: 0 717 1610 1752 Size: 717 893 142 Fragments arranged by size: 893 717 142 PvuII CAG'CTG Cuts at: 0 268 430 955 1409 1752 Size: 268 162 525 454 343 Fragments arranged by size: 525 454 343 268 162 SalI G'TCGA_C Cuts at: 0 111 142 1752 Size: 111 31 1610 Fragments arranged by size: 1610 111 31 SanDI GG'GwC_CC Cuts at: 0 789 1752 Size: 789 963 SapI GCTCTTCn'nnn_ Cuts at: 0 1531 1752 Size: 1531 221 SbfI CC_TGCA'GG Cuts at: 0 717 1752 Size: 717 1035 SfcI C'TryA_G Cuts at: 0 713 836 1606 1752 Size: 713 123 770 146 Fragments arranged by size: 770 713 146 123 SmlI C'TyrA_G Cuts at: 0 961 1703 1752 Size: 961 742 49 Fragments arranged by size: 961 742 49 StuI AGG'CCT Cuts at: 0 1676 1752 Size: 1676 76 StyI C'CwwG_G Cuts at: 0 345 563 999 1085 1217 1656 1752 Size: 345 218 436 86 132 439 96 Fragments arranged by size: 439 436 345 218 132 96 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 957 1390 1752 Size: 957 433 362 Fragments arranged by size: 957 433 362 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1033 1065 1752 Size: 1033 32 687 Fragments arranged by size: 1033 687 32 Tth111I GACn'n_nGTC Cuts at: 0 484 1156 1752 Size: 484 672 596 Fragments arranged by size: 672 596 484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 407 1752 Size: 407 1345 Enzymes that do cut: AccI AcuI AflII AflIII AhdI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BcgI BciVI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BstAPI BstEII BstXI BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII MluI MmeI MscI MspA1I PasI PpuMI PshAI PsiI PstI PvuII SalI SanDI SapI SbfI SfcI SmlI StuI StyI TaqII TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI SacII ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI ZraI