(Circular) (Six-base) MAPSORT of: pFN21AB9741.seq Check: 732 from: 1 to: 6256 pFN21AB9741 6256 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2169 2169 Size: 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 3599 1937 Size: 349 1313 4594 Fragments arranged by size: 4594 1313 349 Acc65I G'GTAC_C Cuts at: 2549 3577 2549 Size: 1028 5228 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3100 4319 5703 5971 1632 Size: 1468 1219 1384 268 1917 Fragments arranged by size: 1917 1468 1384 1219 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 2389 4985 2389 Size: 2596 3660 AleI CACnn'nnGTG Cuts at: 1166 2734 2940 3155 1166 Size: 1568 206 215 4267 Fragments arranged by size: 4267 1568 215 206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2201 2958 1184 Size: 1017 757 4482 Fragments arranged by size: 4482 1017 757 AlwNI CAG_nnn'CTG Cuts at: 2419 2608 3339 3999 5571 2419 Size: 189 731 660 1572 3104 Fragments arranged by size: 3104 1572 731 660 189 ApaI G_GGCC'C Cuts at: 2358 2358 Size: 6256 ApaLI G'TGCA_C Cuts at: 2727 4313 5469 2727 Size: 1586 1156 3514 Fragments arranged by size: 3514 1586 1156 ApoI r'AATT_y Cuts at: 1493 2422 2450 3107 3565 3791 3943 6052 1493 Size: 929 28 657 458 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 657 458 226 152 28 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2293 3470 3581 1870 Size: 73 176 174 1177 111 4545 Fragments arranged by size: 4545 1177 176 174 111 73 BamHI G'GATC_C Cuts at: 2214 3586 3953 2214 Size: 1372 367 4517 Fragments arranged by size: 4517 1372 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2181 2549 2981 3092 3157 Size: 325 217 627 394 368 432 111 65 Cuts at: 3157 3310 3577 5032 618 Size: 153 267 1455 1842 Fragments arranged by size: 1842 1455 627 432 394 368 325 267 217 153 111 65 BanII G_rGCy'C Cuts at: 1391 2358 2922 3058 3216 3243 3290 3399 3477 Size: 967 564 136 158 27 47 109 78 Cuts at: 3477 3575 1391 Size: 98 4072 Fragments arranged by size: 4072 967 564 158 136 109 98 78 47 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 2871 1548 Size: 1323 4933 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2121 2231 2720 Size: 107 122 71 631 605 449 110 489 Cuts at: 2720 4867 136 Size: 2147 1525 Fragments arranged by size: 2147 1525 631 605 489 449 122 110 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3182 3673 3182 Size: 491 5765 Bme1580I G_kGCm'C Cuts at: 1790 2126 2358 2731 2772 4317 5473 1790 Size: 336 232 373 41 1545 1156 2573 Fragments arranged by size: 2573 1545 1156 373 336 232 41 BmgBI CAC'GTC Cuts at: 1318 3199 3262 3319 1318 Size: 1881 63 57 4255 Fragments arranged by size: 4255 1881 63 57 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3987 4945 442 Size: 416 53 83 399 24 2570 958 1753 Fragments arranged by size: 2570 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2326 2428 3161 3230 3521 4916 1239 Size: 1087 102 733 69 291 1395 2579 Fragments arranged by size: 2579 1395 1087 733 291 102 69 Bpu10I CC'TnA_GC Cuts at: 2060 2060 Size: 6256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 4381 5246 5544 3227 Size: 1154 865 298 3939 Fragments arranged by size: 3939 1154 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2653 2686 4919 882 Size: 617 1154 33 2233 2219 Fragments arranged by size: 2233 2219 1154 617 33 BsaAI yAC'GTr Cuts at: 493 1204 4047 493 Size: 711 2843 2702 Fragments arranged by size: 2843 2702 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3465 3486 4063 4445 Size: 53 83 186 936 1932 21 577 382 Cuts at: 4445 5080 275 Size: 635 1451 Fragments arranged by size: 1932 1451 936 635 577 382 186 83 53 21 BsaWI w'CCGG_w Cuts at: 3154 4689 5087 5361 5508 5919 3154 Size: 1535 398 274 147 411 3491 Fragments arranged by size: 3491 1535 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2848 2878 1178 Size: 30 165 30 1445 30 4556 Fragments arranged by size: 4556 1445 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6256 BseYI C'CCAG_C Cuts at: 2545 2928 5459 5955 2545 Size: 383 2531 496 2846 Fragments arranged by size: 2846 2531 496 383 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2824 2849 2918 1163 Size: 573 1088 25 69 4501 Fragments arranged by size: 4501 1088 573 69 25 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3452 3706 4467 5495 1540 Size: 275 186 1451 254 761 1028 2301 Fragments arranged by size: 2301 1451 1028 761 275 254 186 BsiHKAI G_wGCw'C Cuts at: 2151 2731 3216 4317 4402 5473 2151 Size: 580 485 1101 85 1071 2934 Fragments arranged by size: 2934 1101 1071 580 485 85 BsmI GAATG_Cn' Cuts at: 2945 3767 3860 2945 Size: 822 93 5341 Fragments arranged by size: 5341 822 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2402 2402 Size: 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2151 2358 2731 2772 2922 3058 Size: 399 336 25 207 373 41 150 136 Cuts at: 3058 3216 3243 3290 3399 3477 3575 4317 4402 Size: 158 27 47 109 78 98 742 85 Cuts at: 4402 5473 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 373 336 207 158 150 136 109 98 85 78 47 41 27 25 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 3611 844 Size: 1325 1442 3489 Fragments arranged by size: 3489 1442 1325 BsrBI CCG'CTC Cuts at: 1394 1823 3186 4143 1394 Size: 429 1363 957 3507 Fragments arranged by size: 3507 1363 957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2849 3145 4900 5087 1700 Size: 110 1039 296 1755 187 2869 Fragments arranged by size: 2869 1755 1039 296 187 110 BsrGI T'GTAC_A Cuts at: 96 2472 3279 96 Size: 2376 807 3073 Fragments arranged by size: 3073 2376 807 BssHII G'CGCG_C Cuts at: 1925 3352 3385 1925 Size: 1427 33 4796 Fragments arranged by size: 4796 1427 33 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2845 3455 1184 Size: 1661 610 3985 Fragments arranged by size: 3985 1661 610 BstXI CCAn_nnnn'nTGG Cuts at: 3379 3379 Size: 6256 BstYI r'GATC_y Cuts at: 1972 2214 3586 3953 4338 4355 5807 5893 5905 Size: 242 1372 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1372 385 367 346 242 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2358 3217 3290 513 Size: 552 939 354 859 73 3479 Fragments arranged by size: 3479 939 859 552 354 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 3772 4566 4586 3772 Size: 794 20 5442 Fragments arranged by size: 5442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2721 3201 3449 Size: 54 1425 50 275 18 891 480 248 Cuts at: 3449 3703 4592 6221 8 Size: 254 889 1629 43 Fragments arranged by size: 1629 1425 891 889 480 275 254 248 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3449 3703 1537 Size: 275 1637 254 4090 Fragments arranged by size: 4090 1637 275 254 EarI CTCTTCn'nnn_ Cuts at: 1353 2660 3404 3548 4186 1353 Size: 1307 744 144 638 3423 Fragments arranged by size: 3423 1307 744 638 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2125 4843 5217 5363 387 Size: 1064 138 536 2718 374 146 1280 Fragments arranged by size: 2718 1280 1064 536 374 146 138 EcoICRI GAG'CTC Cuts at: 3214 3214 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2326 2428 3100 3161 3230 3521 4319 Size: 393 694 102 672 61 69 291 798 Cuts at: 4319 4916 5703 5971 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 798 787 694 672 597 393 291 268 102 69 61 EcoNI CCTnn'n_nnAGG Cuts at: 2570 2570 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 2297 2354 2712 2297 Size: 57 358 5841 Fragments arranged by size: 5841 358 57 EcoRI G'AATT_C Cuts at: 2450 3565 3943 2450 Size: 1115 378 4763 Fragments arranged by size: 4763 1115 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2281 Size: 6256 FspI TGC'GCA Cuts at: 2177 4762 2177 Size: 2585 3671 HaeII r_GCGC'y Cuts at: 1678 2403 3346 5403 1678 Size: 725 943 2057 2531 Fragments arranged by size: 2531 2057 943 725 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 2756 2788 3496 Size: 32 135 32 803 32 349 32 708 Cuts at: 3496 3528 4897 4929 4971 5003 1373 Size: 32 1369 32 42 32 2626 Fragments arranged by size: 2626 1369 803 708 349 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KpnI G_GTAC'C Cuts at: 2553 3581 2553 Size: 1028 5228 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2723 3203 10 Size: 54 1425 343 891 480 3063 Fragments arranged by size: 3063 1425 891 480 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2734 2940 3155 4214 4573 4732 518 Size: 648 1568 206 215 1059 359 159 2042 Fragments arranged by size: 2042 1568 1059 648 359 215 206 159 MspA1I CmG'CkG Cuts at: 1606 2089 2932 3292 3672 4349 5497 5742 1606 Size: 483 843 360 380 677 1148 245 2120 Fragments arranged by size: 2120 1148 843 677 483 380 360 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2225 3614 5159 1132 Size: 97 996 1389 1545 2229 Fragments arranged by size: 2229 1545 1389 996 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6256 PfoI T'CCnGG_A Cuts at: 3498 3498 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2246 2278 4325 4357 5838 5870 2246 Size: 32 2047 32 1481 32 2632 Fragments arranged by size: 2632 2047 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 2354 2354 Size: 6256 PstI C_TGCA'G Cuts at: 830 2070 2094 2190 3335 3608 830 Size: 1240 24 96 1145 273 3478 Fragments arranged by size: 3478 1240 1145 273 96 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2089 2932 2089 Size: 843 5413 SacI G_AGCT'C Cuts at: 3216 3216 Size: 6256 SacII CC_GC'GG Cuts at: 3293 3293 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SapI GCTCTTCn'nnn_ Cuts at: 3404 3404 Size: 6256 SbfI CC_TGCA'GG Cuts at: 2070 3608 2070 Size: 1538 4718 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SexAI A'CCwGG_T Cuts at: 2132 2132 Size: 6256 SfcI C'TryA_G Cuts at: 826 1045 2066 2090 2186 3331 3604 4739 5420 Size: 219 1021 24 96 1145 273 1135 681 Cuts at: 5420 5611 826 Size: 191 1471 Fragments arranged by size: 1471 1145 1135 1021 681 273 219 191 96 24 SfiI GGCCn_nnn'nGGCC Cuts at: 2720 2720 Size: 6256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2121 3583 1872 Size: 249 1462 4545 Fragments arranged by size: 4545 1462 249 SmlI C'TyrA_G Cuts at: 820 1017 1943 3242 4360 5261 5523 820 Size: 197 926 1299 1118 901 262 1553 Fragments arranged by size: 1553 1299 1118 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 2225 3614 1132 Size: 1093 1389 3774 Fragments arranged by size: 3774 1389 1093 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 3026 1517 Size: 1509 4747 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 2779 3039 3695 513 Size: 552 939 222 553 260 656 3074 Fragments arranged by size: 3074 939 656 553 552 260 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4283 4300 4453 4638 5083 325 Size: 1255 2703 17 153 185 445 1498 Fragments arranged by size: 2703 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 3279 4502 Size: 274 80 33 51 1443 495 807 1223 Cuts at: 4502 6214 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1223 807 495 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2848 2880 6216 6248 2848 Size: 32 3336 32 2856 Fragments arranged by size: 3336 2856 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2134 1316 Size: 818 5438 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2762 3007 2762 Size: 245 6011 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 2119 3581 1870 Size: 249 1462 4545 Fragments arranged by size: 4545 1462 249 XmnI GAAnn'nnTTC Cuts at: 2261 4385 2261 Size: 2124 4132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SfoI SrfI SwaI