(Linear) (Six-base) MAPSORT of: KIEE1946.seq Check: 5864 from: 1 to: 1503 KIEE1946.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1399 1503 Size: 1399 104 Acc65I G'GTAC_C Cuts at: 0 35 1503 Size: 35 1468 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 156 457 808 1503 Size: 156 301 351 695 Fragments arranged by size: 695 351 301 156 AflII C'TTAA_G Cuts at: 0 1186 1503 Size: 1186 317 AflIII A'CryG_T Cuts at: 0 665 1503 Size: 665 838 AhdI GACnn_n'nnGTC Cuts at: 0 1374 1503 Size: 1374 129 AlwNI CAG_nnn'CTG Cuts at: 0 415 1353 1503 Size: 415 938 150 Fragments arranged by size: 938 415 150 ApaI G_GGCC'C Cuts at: 0 461 1146 1503 Size: 461 685 357 Fragments arranged by size: 685 461 357 ApoI r'AATT_y Cuts at: 0 58 1503 Size: 58 1445 AvaI C'yCGr_G Cuts at: 0 454 1372 1503 Size: 454 918 131 Fragments arranged by size: 918 454 131 BanI G'GyrC_C Cuts at: 0 35 79 235 396 1503 Size: 35 44 156 161 1107 Fragments arranged by size: 1107 161 156 44 35 BanII G_rGCy'C Cuts at: 0 455 461 473 1146 1328 1503 Size: 455 6 12 673 182 175 Fragments arranged by size: 673 455 182 175 12 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 162 1503 Size: 162 1341 BglI GCCn_nnn'nGGC Cuts at: 0 809 1150 1407 1503 Size: 809 341 257 96 Fragments arranged by size: 809 341 257 96 Bme1580I G_kGCm'C Cuts at: 0 84 461 684 1146 1403 1503 Size: 84 377 223 462 257 100 Fragments arranged by size: 462 377 257 223 100 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 952 958 1342 1503 Size: 706 246 6 384 161 Fragments arranged by size: 706 384 246 161 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1285 1503 Size: 1285 218 BsaI GGTCTCn'nnnn_ Cuts at: 0 93 1503 Size: 93 1410 BsaAI yAC'GTr Cuts at: 0 666 1503 Size: 666 837 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 1503 Size: 7 1496 BseYI C'CCAG_C Cuts at: 0 421 702 782 805 869 920 1174 1238 Size: 421 281 80 23 64 51 254 64 Cuts at: 1238 1503 Size: 265 Fragments arranged by size: 421 281 265 254 80 64 64 51 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 1503 Size: 51 1452 BsiEI CG_ry'CG Cuts at: 0 813 990 1503 Size: 813 177 513 Fragments arranged by size: 813 513 177 Bsp1286I G_dGCh'C Cuts at: 0 84 455 461 473 684 1146 1328 1403 Size: 84 371 6 12 211 462 182 75 Cuts at: 1403 1503 Size: 100 Fragments arranged by size: 462 371 211 182 100 84 75 12 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 993 1399 1503 Size: 993 406 104 Fragments arranged by size: 993 406 104 BsrBI CCG'CTC Cuts at: 0 1082 1503 Size: 1082 421 BsrDI GCAATG_nn' Cuts at: 0 1306 1503 Size: 1306 197 BsrFI r'CCGG_y Cuts at: 0 812 819 1503 Size: 812 7 684 Fragments arranged by size: 812 684 7 BsrGI T'GTAC_A Cuts at: 0 527 1503 Size: 527 976 BssSI C'ACGA_G Cuts at: 0 464 508 1009 1503 Size: 464 44 501 494 Fragments arranged by size: 501 494 464 44 BstXI CCAn_nnnn'nTGG Cuts at: 0 1452 1503 Size: 1452 51 BstYI r'GATC_y Cuts at: 0 412 719 862 1503 Size: 412 307 143 641 Fragments arranged by size: 641 412 307 143 BtgI C'CryG_G Cuts at: 0 644 962 972 1364 1503 Size: 644 318 10 392 139 Fragments arranged by size: 644 392 318 139 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1239 1503 Size: 1239 264 BtsI GCAGTG_nn' Cuts at: 0 1210 1281 1503 Size: 1210 71 222 Fragments arranged by size: 1210 222 71 EaeI y'GGCC_r Cuts at: 0 125 593 746 810 1035 1086 1151 1503 Size: 125 468 153 64 225 51 65 352 Fragments arranged by size: 468 352 225 153 125 65 64 51 EagI C'GGCC_G Cuts at: 0 810 1503 Size: 810 693 EarI CTCTTCn'nnn_ Cuts at: 0 901 1435 1503 Size: 901 534 68 Fragments arranged by size: 901 534 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 156 457 706 808 952 958 1342 1503 Size: 156 301 249 102 144 6 384 161 Fragments arranged by size: 384 301 249 161 156 144 102 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 688 1503 Size: 688 815 EcoO109I rG'GnC_Cy Cuts at: 0 149 458 640 1017 1053 1143 1503 Size: 149 309 182 377 36 90 360 Fragments arranged by size: 377 360 309 182 149 90 36 EcoRI G'AATT_C Cuts at: 0 58 1503 Size: 58 1445 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 397 1503 Size: 397 1106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 826 858 1360 1392 1503 Size: 826 32 502 32 111 Fragments arranged by size: 826 502 111 32 32 HincII GTy'rAC Cuts at: 0 1221 1503 Size: 1221 282 KpnI G_GTAC'C Cuts at: 0 39 1503 Size: 39 1464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 560 1183 1503 Size: 560 623 320 Fragments arranged by size: 623 560 320 MscI TGG'CCA Cuts at: 0 127 595 1088 1153 1503 Size: 127 468 493 65 350 Fragments arranged by size: 493 468 350 127 65 MslI CAynn'nnrTG Cuts at: 0 191 1480 1503 Size: 191 1289 23 Fragments arranged by size: 1289 191 23 MspA1I CmG'CkG Cuts at: 0 757 786 800 809 924 1128 1503 Size: 757 29 14 9 115 204 375 Fragments arranged by size: 757 375 204 115 29 14 9 NaeI GCC'GGC Cuts at: 0 814 821 1503 Size: 814 7 682 Fragments arranged by size: 814 682 7 NcoI C'CATG_G Cuts at: 0 1364 1503 Size: 1364 139 NgoMIV G'CCGG_C Cuts at: 0 812 819 1503 Size: 812 7 684 Fragments arranged by size: 812 684 7 NspI r_CATG'y Cuts at: 0 519 1219 1503 Size: 519 700 284 Fragments arranged by size: 700 519 284 PasI CC'CwG_GG Cuts at: 0 105 1503 Size: 105 1398 PfoI T'CCnGG_A Cuts at: 0 384 1503 Size: 384 1119 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1102 1134 1503 Size: 1102 32 369 Fragments arranged by size: 1102 369 32 PpuMI rG'GwC_Cy Cuts at: 0 640 1053 1503 Size: 640 413 450 Fragments arranged by size: 640 450 413 PshAI GACnn'nnGTC Cuts at: 0 638 1503 Size: 638 865 PspOMI G'GGCC_C Cuts at: 0 457 1142 1503 Size: 457 685 361 Fragments arranged by size: 685 457 361 PstI C_TGCA'G Cuts at: 0 1201 1501 1503 Size: 1201 300 2 Fragments arranged by size: 1201 300 2 PvuII CAG'CTG Cuts at: 0 786 800 924 1503 Size: 786 14 124 579 Fragments arranged by size: 786 579 124 14 SapI GCTCTTCn'nnn_ Cuts at: 0 901 1503 Size: 901 602 SfcI C'TryA_G Cuts at: 0 350 1197 1497 1503 Size: 350 847 300 6 Fragments arranged by size: 847 350 300 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1150 1503 Size: 1150 353 SmaI CCC'GGG Cuts at: 0 456 1503 Size: 456 1047 SmlI C'TyrA_G Cuts at: 0 1186 1264 1503 Size: 1186 78 239 Fragments arranged by size: 1186 239 78 SrfI GCCC'GGGC Cuts at: 0 456 1503 Size: 456 1047 StuI AGG'CCT Cuts at: 0 254 854 943 1503 Size: 254 600 89 560 Fragments arranged by size: 600 560 254 89 StyI C'CwwG_G Cuts at: 0 140 473 1364 1503 Size: 140 333 891 139 Fragments arranged by size: 891 333 140 139 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 67 650 1401 1503 Size: 67 583 751 102 Fragments arranged by size: 751 583 102 67 TatI w'GTAC_w Cuts at: 0 512 527 1503 Size: 512 15 976 Fragments arranged by size: 976 512 15 Tth111I GACn'n_nGTC Cuts at: 0 537 1477 1503 Size: 537 940 26 Fragments arranged by size: 940 537 26 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 120 1503 Size: 120 1383 XmaI C'CCGG_G Cuts at: 0 454 1503 Size: 454 1049 XmnI GAAnn'nnTTC Cuts at: 0 169 895 1503 Size: 169 726 608 Fragments arranged by size: 726 608 169 Enzymes that do cut: AarI Acc65I AcuI AflII AflIII AhdI AlwNI ApaI ApoI AvaI BanI BanII BbsI BglI Bme1580I BpmI BpuEI BsaI BsaAI BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SapI SfcI SfiI SmaI SmlI SrfI StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI XbaI XhoI ZraI