Restriction map for pFN21AB7743
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AgeI        Alw44I      ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AviII       AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BfaI        BlnI        
BsaWI       BseAI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CpoI        DraI        
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HindIII     HpaI        KpnI        MaeI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    RcaI        RsrII       SacI        SbfI        
ScaI        SexAI       SfiI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp509I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          451  GTCGAC               GT^[AC][GT]AC
AflII         856  CTTAAG               C^TTAAG
AflIII        739  ACGTGT               A^C[GA][CT]GT
AhdI         1114  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       410  AGCGCT               AGC^GCT
ApaI           14  GGGCCC               GGGCC^C
AspEI        1114  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          442  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          927  GAAGAC               GAAGAC(2/6)
BclI          791  TGATCA               T^GATCA
BfrI          856  CTTAAG               C^TTAAG
BglI          130  GCCCCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         848  AGATCT               A^GATCT
BlpI          426  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      426  GCTGAGC              GC^T[ACGT]AGC
BpuAI         927  GAAGAC               GAAGAC(2/6)
BsaAI         733  TACGTG               [CT]AC^GT[GA]
BsgI          484  GTGCAG               GTGCAG(16/14)
BspDI         877  ATCGAT               AT^CGAT
BspMI         185  ACCTGC               ACCTGC(4/8)
BssSI        1216  CACGAG               CACGAG(-5/-1)
BstAPI        763  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         426  GCTGAGC              GC^T[ACGT]AGC
ClaI          877  ATCGAT               AT^CGAT
DraIII        224  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          442  GACGGTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1114  GACCGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      410  AGCGCT               AGC^GCT
EcoT14I         5  CCTTGG               C^C[AT][AT]GG
FbaI          791  TGATCA               T^GATCA
MseI          857  TTAA                 T^TAA
NspI         1155  GCATGT               [GA]CATG^[CT]
PaeR7I       1243  CTCGAG               C^TCGAG
PflFI         442  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI          875  CGATCG               CGAT^CG
SalI          451  GTCGAC               G^TCGAC
SapI          192  GCTCTTC              GCTCTTC(1/4)
SmaI          363  CCCGGG               CCC^GGG
StyI            5  CCTTGG               C^C[AT][AT]GG
TfiI          396  GAATC                G^A[AT]TC
Tru9I         857  TTAA                 T^TAA
Tth111I       442  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          407  CCAAGCGCTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1243  CTCGAG               C^TCGAG
XmaCI         363  CCCGGG               C^CCGGG
XmaI          363  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AfaI          297  GTAC                 GT^AC
              680  GTAC                
AlwI         1033  GATCC                GGATC(4/5)
              914  GGATC               
AocI          895  CCTGAGG              CC^T[ACGT]AGG
             1186  CCTGAGG             
BanI          156  GGCGCC               G^G[CT][GA]CC
              210  GGCGCC              
BanII          14  GGGCCC               G[GA]GC[CT]^C
             1097  GGGCTC              
BbeI          156  GGCGCC               GGCGC^C
              210  GGCGCC              
BsaBI          96  GATCGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              669  GATGAATATC          
BsrDI         537  CATTGC               GCAATG(2/0)
              759  CATTGC              
BssHII         35  GCGCGC               G^CGCGC
               68  GCGCGC              
BstYI         848  AGATCT               [GA]^GATC[CT]
             1032  AGATCC              
Bsu36I        895  CCTGAGG              CC^T[ACGT]AGG
             1186  CCTGAGG             
EagI          249  CGGCCG               C^GGCCG
             1136  CGGCCG              
EclXI         249  CGGCCG               C^GGCCG
             1136  CGGCCG              
Eco52I        249  CGGCCG               C^GGCCG
             1136  CGGCCG              
Eco81I        895  CCTGAGG              CC^T[ACGT]AGG
             1186  CCTGAGG             
EcoNI        1182  CCTCCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1242  CCTCGAGGAGG         
HgaI          121  GACGC                GACGC(5/10)
              593  GCGTC               
HincII        451  GTCGAC               GT[CT]^[GA]AC
              615  GTTGAC              
HindII        451  GTCGAC               GT[CT]^[GA]AC
              615  GTTGAC              
KasI          156  GGCGCC               G^GCGCC
              210  GGCGCC              
KspI          152  CCGCGG               CCGC^GG
              235  CCGCGG              
MamI           96  GATCGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              669  GATGAATATC          
MflI          848  AGATCT               [GA]^GATC[CT]
             1032  AGATCC              
MslI          582  CACCACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              984  CACCATAGTG          
NarI          156  GGCGCC               GG^CGCC
              210  GGCGCC              
NlaIII         32  CATG                 CATG^
             1156  CATG                
PstI          637  CTGCAG               CTGCA^G
              770  CTGCAG              
RsaI          297  GTAC                 GT^AC
              680  GTAC                
SacII         152  CCGCGG               CCGC^GG
              235  CCGCGG              
SfcI          637  CTGCAG               C^T[GA][CT]AG
              770  CTGCAG              
SfoI          156  GGCGCC               GGC^GCC
              210  GGCGCC              
SgrAI          38  CGCCGGCG             C[GA]^CCGG[CT]G
              257  CGCCGGTG            
XhoII         848  AGATCT               [GA]^GATC[CT]
             1032  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
AvaI        AvaII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsiEI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIV       PflMI       
PleI        PpuMI       PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      
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