Restriction map for
pFN21AB7743
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BfaI BlnI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CpoI DraI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI MaeI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SacI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 451 GTCGAC GT^[AC][GT]AC
AflII 856 CTTAAG C^TTAAG
AflIII 739 ACGTGT A^C[GA][CT]GT
AhdI 1114 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 410 AGCGCT AGC^GCT
ApaI 14 GGGCCC GGGCC^C
AspEI 1114 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 442 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 927 GAAGAC GAAGAC(2/6)
BclI 791 TGATCA T^GATCA
BfrI 856 CTTAAG C^TTAAG
BglI 130 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 848 AGATCT A^GATCT
BlpI 426 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 426 GCTGAGC GC^T[ACGT]AGC
BpuAI 927 GAAGAC GAAGAC(2/6)
BsaAI 733 TACGTG [CT]AC^GT[GA]
BsgI 484 GTGCAG GTGCAG(16/14)
BspDI 877 ATCGAT AT^CGAT
BspMI 185 ACCTGC ACCTGC(4/8)
BssSI 1216 CACGAG CACGAG(-5/-1)
BstAPI 763 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 426 GCTGAGC GC^T[ACGT]AGC
ClaI 877 ATCGAT AT^CGAT
DraIII 224 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 442 GACGGTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1114 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 410 AGCGCT AGC^GCT
EcoT14I 5 CCTTGG C^C[AT][AT]GG
FbaI 791 TGATCA T^GATCA
MseI 857 TTAA T^TAA
NspI 1155 GCATGT [GA]CATG^[CT]
PaeR7I 1243 CTCGAG C^TCGAG
PflFI 442 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 875 CGATCG CGAT^CG
SalI 451 GTCGAC G^TCGAC
SapI 192 GCTCTTC GCTCTTC(1/4)
SmaI 363 CCCGGG CCC^GGG
StyI 5 CCTTGG C^C[AT][AT]GG
TfiI 396 GAATC G^A[AT]TC
Tru9I 857 TTAA T^TAA
Tth111I 442 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 407 CCAAGCGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1243 CTCGAG C^TCGAG
XmaCI 363 CCCGGG C^CCGGG
XmaI 363 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 297 GTAC GT^AC
680 GTAC
AlwI 1033 GATCC GGATC(4/5)
914 GGATC
AocI 895 CCTGAGG CC^T[ACGT]AGG
1186 CCTGAGG
BanI 156 GGCGCC G^G[CT][GA]CC
210 GGCGCC
BanII 14 GGGCCC G[GA]GC[CT]^C
1097 GGGCTC
BbeI 156 GGCGCC GGCGC^C
210 GGCGCC
BsaBI 96 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
669 GATGAATATC
BsrDI 537 CATTGC GCAATG(2/0)
759 CATTGC
BssHII 35 GCGCGC G^CGCGC
68 GCGCGC
BstYI 848 AGATCT [GA]^GATC[CT]
1032 AGATCC
Bsu36I 895 CCTGAGG CC^T[ACGT]AGG
1186 CCTGAGG
EagI 249 CGGCCG C^GGCCG
1136 CGGCCG
EclXI 249 CGGCCG C^GGCCG
1136 CGGCCG
Eco52I 249 CGGCCG C^GGCCG
1136 CGGCCG
Eco81I 895 CCTGAGG CC^T[ACGT]AGG
1186 CCTGAGG
EcoNI 1182 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1242 CCTCGAGGAGG
HgaI 121 GACGC GACGC(5/10)
593 GCGTC
HincII 451 GTCGAC GT[CT]^[GA]AC
615 GTTGAC
HindII 451 GTCGAC GT[CT]^[GA]AC
615 GTTGAC
KasI 156 GGCGCC G^GCGCC
210 GGCGCC
KspI 152 CCGCGG CCGC^GG
235 CCGCGG
MamI 96 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
669 GATGAATATC
MflI 848 AGATCT [GA]^GATC[CT]
1032 AGATCC
MslI 582 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
984 CACCATAGTG
NarI 156 GGCGCC GG^CGCC
210 GGCGCC
NlaIII 32 CATG CATG^
1156 CATG
PstI 637 CTGCAG CTGCA^G
770 CTGCAG
RsaI 297 GTAC GT^AC
680 GTAC
SacII 152 CCGCGG CCGC^GG
235 CCGCGG
SfcI 637 CTGCAG C^T[GA][CT]AG
770 CTGCAG
SfoI 156 GGCGCC GGC^GCC
210 GGCGCC
SgrAI 38 CGCCGGCG C[GA]^CCGG[CT]G
257 CGCCGGTG
XhoII 848 AGATCT [GA]^GATC[CT]
1032 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIV PflMI
PleI PpuMI PvuII Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TseI
Tsp45I TspRI TthHB8I Van91I