Restriction map for
pFN21AB7743
(Linear) (Six-base) MAPSORT of: KIBB7743.seq Check: 9116 from: 1 to: 1311
KIBB7743.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 452 1311
Size: 452 859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1311
Size: 499 812
AfeI AGC'GCT
Cuts at: 0 412 1311
Size: 412 899
AflII C'TTAA_G
Cuts at: 0 856 1311
Size: 856 455
AflIII A'CryG_T
Cuts at: 0 739 1311
Size: 739 572
AhdI GACnn_n'nnGTC
Cuts at: 0 1119 1311
Size: 1119 192
AleI CACnn'nnGTG
Cuts at: 0 586 988 1311
Size: 586 402 323
Fragments arranged by size:
586 402 323
AlwNI CAG_nnn'CTG
Cuts at: 0 300 570 657 726 769 1311
Size: 300 270 87 69 43 542
Fragments arranged by size:
542 300 270 87 69 43
ApaI G_GGCC'C
Cuts at: 0 18 1311
Size: 18 1293
AvaI C'yCGr_G
Cuts at: 0 58 323 363 1243 1311
Size: 58 265 40 880 68
Fragments arranged by size:
880 265 68 58 40
BanI G'GyrC_C
Cuts at: 0 156 210 1311
Size: 156 54 1101
Fragments arranged by size:
1101 156 54
BanII G_rGCy'C
Cuts at: 0 18 1101 1311
Size: 18 1083 210
Fragments arranged by size:
1083 210 18
BbeI G_GCGC'C
Cuts at: 0 160 214 1311
Size: 160 54 1097
Fragments arranged by size:
1097 160 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 934 1311
Size: 934 377
BclI T'GATC_A
Cuts at: 0 791 1311
Size: 791 520
BglI GCCn_nnn'nGGC
Cuts at: 0 136 1311
Size: 136 1175
BglII A'GATC_T
Cuts at: 0 848 1311
Size: 848 463
BlpI GC'TnA_GC
Cuts at: 0 427 1311
Size: 427 884
Bme1580I G_kGCm'C
Cuts at: 0 18 895 1311
Size: 18 877 416
Fragments arranged by size:
877 416 18
BmgBI CAC'GTC
Cuts at: 0 740 1311
Size: 740 571
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 471 1311
Size: 221 250 840
Fragments arranged by size:
840 250 221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 1311
Size: 1188 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 439 1078 1171 1311
Size: 67 372 639 93 140
Fragments arranged by size:
639 372 140 93 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 628 1311
Size: 541 87 683
Fragments arranged by size:
683 541 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 802 895 1259 1311
Size: 379 423 93 364 52
Fragments arranged by size:
423 379 364 93 52
BsaAI yAC'GTr
Cuts at: 0 735 1311
Size: 735 576
BsaBI GATnn'nnATC
Cuts at: 0 100 673 1311
Size: 100 573 638
Fragments arranged by size:
638 573 100
BsaHI Gr'CG_yC
Cuts at: 0 122 157 211 593 1311
Size: 122 35 54 382 718
Fragments arranged by size:
718 382 122 54 35
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 913 970 973 1171 1204 1261 1311
Size: 78 835 57 3 198 33 57 50
Fragments arranged by size:
835 198 78 57 57 50 33 3
BseYI C'CCAG_C
Cuts at: 0 570 812 1036 1311
Size: 570 242 224 275
Fragments arranged by size:
570 275 242 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1311
Size: 505 806
BsiEI CG_ry'CG
Cuts at: 0 252 878 1139 1311
Size: 252 626 261 172
Fragments arranged by size:
626 261 252 172
Bsp1286I G_dGCh'C
Cuts at: 0 18 895 1101 1311
Size: 18 877 206 210
Fragments arranged by size:
877 210 206 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 1311
Size: 194 1117
BsrDI GCAATG_nn'
Cuts at: 0 536 758 1311
Size: 536 222 553
Fragments arranged by size:
553 536 222
BsrFI r'CCGG_y
Cuts at: 0 39 64 135 145 166 258 1311
Size: 39 25 71 10 21 92 1053
Fragments arranged by size:
1053 92 71 39 25 21 10
BssHII G'CGCG_C
Cuts at: 0 35 68 1311
Size: 35 33 1243
Fragments arranged by size:
1243 35 33
BssSI C'ACGA_G
Cuts at: 0 1216 1311
Size: 1216 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 769 1311
Size: 769 542
BstYI r'GATC_y
Cuts at: 0 848 1032 1311
Size: 848 184 279
Fragments arranged by size:
848 279 184
Bsu36I CC'TnA_GG
Cuts at: 0 896 1187 1311
Size: 896 291 124
Fragments arranged by size:
896 291 124
BtgI C'CryG_G
Cuts at: 0 152 235 824 1103 1311
Size: 152 83 589 279 208
Fragments arranged by size:
589 279 208 152 83
BtsI GCAGTG_nn'
Cuts at: 0 318 1311
Size: 318 993
ClaI AT'CG_AT
Cuts at: 0 878 1311
Size: 878 433
DraIII CAC_nnn'GTG
Cuts at: 0 229 1311
Size: 229 1082
DrdI GACnn_nn'nnGTC
Cuts at: 0 448 1311
Size: 448 863
EaeI y'GGCC_r
Cuts at: 0 8 249 302 1136 1311
Size: 8 241 53 834 175
Fragments arranged by size:
834 241 175 53 8
EagI C'GGCC_G
Cuts at: 0 249 1136 1311
Size: 249 887 175
Fragments arranged by size:
887 249 175
EarI CTCTTCn'nnn_
Cuts at: 0 199 837 1276 1311
Size: 199 638 439 35
Fragments arranged by size:
638 439 199 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 99 614 1311
Size: 99 515 697
Fragments arranged by size:
697 515 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 439 499 1078 1171 1311
Size: 67 372 60 579 93 140
Fragments arranged by size:
579 372 140 93 67 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 1246 1311
Size: 1186 60 65
Fragments arranged by size:
1186 65 60
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 51 624 747 1221 1290 1311
Size: 14 1 36 573 123 474 69 21
Fragments arranged by size:
573 474 123 69 36 21 14 1
HaeII r_GCGC'y
Cuts at: 0 46 131 160 214 414 726 1311
Size: 46 85 29 54 200 312 585
Fragments arranged by size:
585 312 200 85 54 46 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1311
Size: 1105 32 174
Fragments arranged by size:
1105 174 32
HincII GTy'rAC
Cuts at: 0 453 617 1311
Size: 453 164 694
Fragments arranged by size:
694 453 164
KasI G'GCGC_C
Cuts at: 0 156 210 1311
Size: 156 54 1101
Fragments arranged by size:
1101 156 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1311
Size: 654 657
MslI CAynn'nnrTG
Cuts at: 0 586 988 1311
Size: 586 402 323
Fragments arranged by size:
586 402 323
MspA1I CmG'CkG
Cuts at: 0 154 237 636 657 690 1071 1128 1311
Size: 154 83 399 21 33 381 57 183
Fragments arranged by size:
399 381 183 154 83 57 33 21
NaeI GCC'GGC
Cuts at: 0 41 66 137 168 1311
Size: 41 25 71 31 1143
Fragments arranged by size:
1143 71 41 31 25
NarI GG'CG_CC
Cuts at: 0 157 211 1311
Size: 157 54 1100
Fragments arranged by size:
1100 157 54
NgoMIV G'CCGG_C
Cuts at: 0 39 64 135 166 1311
Size: 39 25 71 31 1145
Fragments arranged by size:
1145 71 39 31 25
NspI r_CATG'y
Cuts at: 0 1159 1311
Size: 1159 152
PasI CC'CwG_GG
Cuts at: 0 922 1311
Size: 922 389
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 726 825 1311
Size: 570 156 99 486
Fragments arranged by size:
570 486 156 99
PfoI T'CCnGG_A
Cuts at: 0 342 416 1078 1148 1311
Size: 342 74 662 70 163
Fragments arranged by size:
662 342 163 74 70
PpuMI rG'GwC_Cy
Cuts at: 0 624 747 1290 1311
Size: 624 123 543 21
Fragments arranged by size:
624 543 123 21
PspOMI G'GGCC_C
Cuts at: 0 14 1311
Size: 14 1297
PspXI vC'TCGA_Gb
Cuts at: 0 1243 1311
Size: 1243 68
PstI C_TGCA'G
Cuts at: 0 641 774 1311
Size: 641 133 537
Fragments arranged by size:
641 537 133
PvuI CG_AT'CG
Cuts at: 0 878 1311
Size: 878 433
PvuII CAG'CTG
Cuts at: 0 636 657 690 1311
Size: 636 21 33 621
Fragments arranged by size:
636 621 33 21
SacII CC_GC'GG
Cuts at: 0 155 238 1311
Size: 155 83 1073
Fragments arranged by size:
1073 155 83
SalI G'TCGA_C
Cuts at: 0 451 1311
Size: 451 860
SapI GCTCTTCn'nnn_
Cuts at: 0 199 1311
Size: 199 1112
SfcI C'TryA_G
Cuts at: 0 637 770 1311
Size: 637 133 541
Fragments arranged by size:
637 541 133
SfoI GGC'GCC
Cuts at: 0 158 212 1311
Size: 158 54 1099
Fragments arranged by size:
1099 158 54
SgrAI Cr'CCGG_yG
Cuts at: 0 39 258 1311
Size: 39 219 1053
Fragments arranged by size:
1053 219 39
SmaI CCC'GGG
Cuts at: 0 365 1311
Size: 365 946
SmlI C'TyrA_G
Cuts at: 0 520 607 856 1243 1311
Size: 520 87 249 387 68
Fragments arranged by size:
520 387 249 87 68
StyI C'CwwG_G
Cuts at: 0 5 1311
Size: 5 1306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 1311
Size: 240 1071
TatI w'GTAC_w
Cuts at: 0 679 1311
Size: 679 632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 1017 1049 1311
Size: 338 32 647 32 262
Fragments arranged by size:
647 338 262 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 445 1311
Size: 445 866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 1311
Size: 414 897
XhoI C'TCGA_G
Cuts at: 0 1243 1311
Size: 1243 68
XmaI C'CCGG_G
Cuts at: 0 363 1311
Size: 363 948
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AhdI AleI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI
Bsp1286I BspMI BsrDI BsrFI BssHII BssSI BstAPI BstYI
Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuI
PvuII SacII SalI SapI SfcI SfoI SgrAI SmaI
SmlI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BfrBI BmtI Bpu10I BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BstBI
BstEII BstXI BstZ17I BtgZI CspCI DraI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI RsrII SacI SanDI SbfI ScaI SexAI SfiI
SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XmnI ZraI