(Circular) (Six-base) MAPSORT of: pFN21AE5278.seq Check: 4767 from: 1 to: 5410 pFN21AE5278 5410 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3220 278 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 1937 Size: 816 4594 Acc65I G'GTAC_C Cuts at: 2185 2731 2185 Size: 546 4864 AclI AA'CG_TT Cuts at: 1563 3537 3910 1563 Size: 1974 373 3063 Fragments arranged by size: 3063 1974 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3473 4857 5125 1632 Size: 1841 1384 268 1917 Fragments arranged by size: 1917 1841 1384 268 AfeI AGC'GCT Cuts at: 2462 2525 2462 Size: 63 5347 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5213 AflIII A'CryG_T Cuts at: 1203 4309 5286 1203 Size: 3106 977 1327 Fragments arranged by size: 3106 1327 977 AgeI A'CCGG_T Cuts at: 4241 4241 Size: 5410 AhdI GACnn_n'nnGTC Cuts at: 4139 4139 Size: 5410 AleI CACnn'nnGTG Cuts at: 1166 2297 1166 Size: 1131 4279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2286 1184 Size: 1102 4308 AlwNI CAG_nnn'CTG Cuts at: 2134 2602 3153 4725 2134 Size: 468 551 1572 2819 Fragments arranged by size: 2819 1572 551 468 ApaLI G'TGCA_C Cuts at: 2261 3467 4623 2261 Size: 1206 1156 3048 Fragments arranged by size: 3048 1206 1156 ApoI r'AATT_y Cuts at: 1493 2719 2945 3097 5206 1493 Size: 1226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 226 152 AseI AT'TA_AT Cuts at: 160 1032 3964 160 Size: 872 2932 1606 Fragments arranged by size: 2932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 AvaI C'yCGr_G Cuts at: 1870 1943 2619 2735 1870 Size: 73 676 116 4545 Fragments arranged by size: 4545 676 116 73 BamHI G'GATC_C Cuts at: 2740 3107 2740 Size: 367 5043 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 2605 2731 4186 618 Size: 325 217 627 398 420 126 1455 1842 Fragments arranged by size: 1842 1455 627 420 398 325 217 126 BanII G_rGCy'C Cuts at: 1391 2477 2540 2729 1391 Size: 1086 63 189 4072 Fragments arranged by size: 4072 1086 189 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2149 2183 3601 3635 1903 Size: 34 212 34 1418 34 3678 Fragments arranged by size: 3678 1418 212 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3302 4512 3302 Size: 1210 4200 BclI T'GATC_A Cuts at: 1548 1548 Size: 5410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2360 2535 4021 Size: 107 122 71 631 605 688 175 1486 Cuts at: 4021 136 Size: 1525 Fragments arranged by size: 1525 1486 688 631 605 175 122 107 71 BglII A'GATC_T Cuts at: 5405 5405 Size: 5410 BlpI GC'TnA_GC Cuts at: 2827 2827 Size: 5410 Bme1580I G_kGCm'C Cuts at: 1790 2265 3471 4627 1790 Size: 475 1206 1156 2573 Fragments arranged by size: 2573 1206 1156 475 BmgBI CAC'GTC Cuts at: 1318 2344 1318 Size: 1026 4384 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3141 4099 442 Size: 416 53 83 399 24 1724 958 1753 Fragments arranged by size: 1753 1724 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 4070 1239 Size: 1235 1596 2579 Fragments arranged by size: 2579 1596 1235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4400 4698 3535 Size: 865 298 4247 Fragments arranged by size: 4247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4073 882 Size: 617 2574 2219 Fragments arranged by size: 2574 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3201 493 Size: 711 1997 2702 Fragments arranged by size: 2702 1997 711 BsaBI GATnn'nnATC Cuts at: 5404 5404 Size: 5410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3217 3599 4234 275 Size: 53 83 186 936 1684 382 635 1451 Fragments arranged by size: 1684 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3843 4241 4515 4662 5073 3843 Size: 398 274 147 411 4180 Fragments arranged by size: 4180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5185 Fragments arranged by size: 5185 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 2504 2507 2545 1186 Size: 1285 33 3 38 4051 Fragments arranged by size: 4051 1285 38 33 3 BseYI C'CCAG_C Cuts at: 2080 2127 2421 4613 5109 2080 Size: 47 294 2192 496 2381 Fragments arranged by size: 2381 2192 496 294 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4837 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2169 2636 2860 3621 4649 1540 Size: 275 186 168 467 224 761 1028 2301 Fragments arranged by size: 2301 1028 761 467 275 224 186 168 BsiHKAI G_wGCw'C Cuts at: 2265 3471 3556 4627 2265 Size: 1206 85 1071 3048 Fragments arranged by size: 3048 1206 1071 85 BsmI GAATG_Cn' Cuts at: 2921 3014 2921 Size: 93 5317 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2477 2540 2729 3471 3556 4627 Size: 399 475 212 63 189 742 85 1071 Cuts at: 4627 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 475 399 212 189 85 63 BspEI T'CCGG_A Cuts at: 5073 5073 Size: 5410 BspHI T'CATG_A Cuts at: 3299 5029 3299 Size: 1730 3680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2765 844 Size: 1921 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3297 1394 Size: 429 1474 3507 Fragments arranged by size: 3507 1474 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2017 3905 4079 65 Size: 1183 769 1888 174 1396 Fragments arranged by size: 1888 1396 1183 769 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2445 2529 2631 4054 4241 1700 Size: 110 635 84 102 1423 187 2869 Fragments arranged by size: 2869 1423 635 187 110 102 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5410 BssHII G'CGCG_C Cuts at: 1925 2500 2502 2651 1925 Size: 575 2 149 4684 Fragments arranged by size: 4684 575 149 2 BssSI C'ACGA_G Cuts at: 3470 4482 3470 Size: 1012 4398 BstAPI GCAn_nnn'nTGC Cuts at: 794 2526 794 Size: 1732 3678 BstBI TT'CG_AA Cuts at: 4214 4214 Size: 5410 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5410 BstXI CCAn_nnnn'nTGG Cuts at: 2071 2071 Size: 5410 BstYI r'GATC_y Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405 Size: 768 367 385 17 1452 86 12 346 Cuts at: 5405 1972 Size: 1977 Fragments arranged by size: 1977 1452 768 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2181 2181 Size: 5410 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3919 Fragments arranged by size: 3919 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2359 487 Size: 683 56 1133 3538 Fragments arranged by size: 3538 1133 683 56 BtsI GCAGTG_nn' Cuts at: 2227 2263 2926 3720 3740 2227 Size: 36 663 794 20 3897 Fragments arranged by size: 3897 794 663 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5375 DraI TTT'AAA Cuts at: 2714 2714 Size: 5410 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4417 809 Size: 507 3101 1802 Fragments arranged by size: 3101 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2633 2857 3746 Size: 54 1425 50 275 18 803 224 889 Cuts at: 3746 5375 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 803 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2633 2857 1537 Size: 275 821 224 4090 Fragments arranged by size: 4090 821 275 224 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 3340 1353 Size: 1053 934 3423 Fragments arranged by size: 3423 1053 934 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2548 3997 4371 4517 387 Size: 1064 138 959 1449 374 146 1280 Fragments arranged by size: 1449 1280 1064 959 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 3473 4070 4857 5125 1239 Size: 393 842 999 597 787 268 1524 Fragments arranged by size: 1524 999 842 787 597 393 268 EcoRI G'AATT_C Cuts at: 2719 3097 2719 Size: 378 5032 EcoRV GAT'ATC Cuts at: 2146 2146 Size: 5410 FspI TGC'GCA Cuts at: 3916 3916 Size: 5410 HaeII r_GCGC'y Cuts at: 1678 2464 2527 2602 4557 1678 Size: 786 63 75 1955 2531 Fragments arranged by size: 2531 1955 786 75 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 4051 4083 4125 Size: 32 135 32 456 32 1991 32 42 Cuts at: 4125 4157 1373 Size: 32 2626 Fragments arranged by size: 2626 1991 456 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2754 3000 669 Size: 988 281 816 246 3079 Fragments arranged by size: 3079 988 816 281 246 HindIII A'AGCT_T Cuts at: 748 2438 748 Size: 1690 3720 HpaI GTT'AAC Cuts at: 3000 3000 Size: 5410 KpnI G_GTAC'C Cuts at: 2189 2735 2189 Size: 546 4864 MfeI C'AATT_G Cuts at: 3009 3009 Size: 5410 MluI A'CGCG_T Cuts at: 5286 5286 Size: 5410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4524 4708 1305 Size: 3219 184 2007 Fragments arranged by size: 3219 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3588 Fragments arranged by size: 3588 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 3368 3727 3886 518 Size: 648 1131 1071 359 159 2042 Fragments arranged by size: 2042 1131 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2452 2515 2638 2644 2826 3503 4651 Size: 474 372 63 123 6 182 677 1148 Cuts at: 4651 4896 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 474 372 245 182 123 63 6 NaeI GCC'GGC Cuts at: 2447 2531 2447 Size: 84 5326 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3919 Fragments arranged by size: 3919 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5410 NgoMIV G'CCGG_C Cuts at: 2445 2529 2445 Size: 84 5326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5410 NotI GC'GGCC_GC Cuts at: 2857 2857 Size: 5410 NspI r_CATG'y Cuts at: 1132 1229 2768 4313 1132 Size: 97 1539 1545 2229 Fragments arranged by size: 2229 1545 1539 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5410 PciI A'CATG_T Cuts at: 4309 4309 Size: 5410 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5410 PfoI T'CCnGG_A Cuts at: 2032 2032 Size: 5410 PmeI GTTT'AAAC Cuts at: 2714 2714 Size: 5410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3479 3511 4992 5024 3479 Size: 32 1481 32 3865 Fragments arranged by size: 3865 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5410 PsiI TTA'TAA Cuts at: 2980 2980 Size: 5410 PstI C_TGCA'G Cuts at: 830 2142 2598 2762 830 Size: 1312 456 164 3478 Fragments arranged by size: 3478 1312 456 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5410 PvuII CAG'CTG Cuts at: 2080 2452 2080 Size: 372 5038 SalI G'TCGA_C Cuts at: 1936 2752 1936 Size: 816 4594 SbfI CC_TGCA'GG Cuts at: 2762 2762 Size: 5410 ScaI AGT'ACT Cuts at: 3658 5370 3658 Size: 1712 3698 SfcI C'TryA_G Cuts at: 826 1045 2138 2594 2758 3893 4574 4765 826 Size: 219 1093 456 164 1135 681 191 1471 Fragments arranged by size: 1471 1135 1093 681 456 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5410 SmaI CCC'GGG Cuts at: 1872 2621 2737 1872 Size: 749 116 4545 Fragments arranged by size: 4545 749 116 SmlI C'TyrA_G Cuts at: 820 1017 1943 3514 4415 4677 820 Size: 197 926 1571 901 262 1553 Fragments arranged by size: 1571 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3201 493 Size: 2708 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5410 SphI G_CATG'C Cuts at: 1132 2768 1132 Size: 1636 3774 SspI AAT'ATT Cuts at: 5 52 3334 5 Size: 47 3282 2081 Fragments arranged by size: 3282 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5410 StyI C'CwwG_G Cuts at: 513 1065 2004 2022 2849 513 Size: 552 939 18 827 3074 Fragments arranged by size: 3074 939 827 552 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3437 3454 3607 3792 4237 325 Size: 1255 1857 17 153 185 445 1498 Fragments arranged by size: 1857 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3656 5368 96 Size: 274 80 33 51 1443 1679 1712 138 Fragments arranged by size: 1712 1679 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5370 5402 5370 Size: 32 5378 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5410 XbaI T'CTAG_A Cuts at: 2746 2746 Size: 5410 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5410 XmaI C'CCGG_G Cuts at: 1870 2619 2735 1870 Size: 749 116 4545 Fragments arranged by size: 4545 749 116 XmnI GAAnn'nnTTC Cuts at: 3539 3539 Size: 5410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3218 276 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI