Restriction map for
pFN21AE1627
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 198 CCGC CCGC(-3/-1)
AcsI 392 AAATTT [GA]^AATT[CT]
ApoI 392 AAATTT [GA]^AATT[CT]
AseI 175 ATTAAT AT^TAAT
AsnI 175 ATTAAT AT^TAAT
AvaII 83 GGACC G^G[AT]CC
BcgI 274 CGAACCACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 59 CCGGG CC^[GC]GG
BfaI 80 CTAG C^TAG
BmyI 137 GTGCCC G[AGT]GC[ACT]^C
BsgI 130 GTGCAG GTGCAG(16/14)
BsiEI 270 CGGTCG CG[GA][CT]^CG
Bsp1286I 137 GTGCCC G[AGT]GC[ACT]^C
Cac8I 135 GCGTGC GC[ACGT]^[ACGT]GC
DraII 82 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 53 CCGTGG C^C[GA][CT]GG
EarI 346 GAAGAG CTCTTC(1/4)
EcoO109I 82 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 86 CCTTGG C^C[AT][AT]GG
Ksp632I 346 GAAGAG CTCTTC(1/4)
MaeI 80 CTAG C^TAG
MboII 346 GAAGA GAAGA(8/7)
MseI 176 TTAA T^TAA
MwoI 58 GCCGGGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 59 CCGGG CC^[GC]GG
NspI 280 ACATGC [GA]CATG^[CT]
PpuMI 82 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 307 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 175 ATTAAT AT^TAAT
PstI 205 CTGCAG CTGCA^G
ScaI 188 AGTACT AGT^ACT
SfaNI 98 GCATC GCATC(5/9)
StyI 86 CCTTGG C^C[AT][AT]GG
TaqI 273 TCGA T^CGA
Tru9I 176 TTAA T^TAA
Tsp509I 393 AATT ^AATT
TspRI 23 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 273 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 162 GTAC GT^AC
189 GTAC
BsmFI 291 GTCCC GGGAC(10/14)
314 GTCCC
BspMI 91 GCAGGT ACCTGC(4/8)
207 GCAGGT
BstF5I 99 CATCC GGATG(2/0)
183 GGATG
BstNI 141 CCTGG CC^[AT]GG
380 CCTGG
DpnII 231 GATC ^GATC
366 GATC
EaeI 50 TGGCCG [CT]^GGCC[GA]
56 TGGCCG
EcoRII 141 CCTGG ^CC[AT]GG
380 CCTGG
FokI 99 CATCC GGATG(9/13)
183 GGATG
HapII 59 CCGG C^CGG
417 CCGG
HpaII 59 CCGG C^CGG
417 CCGG
HphI 210 GGTGA GGTGA(8/7)
243 GGTGA
MboI 231 GATC ^GATC
366 GATC
MspI 59 CCGG C^CGG
417 CCGG
MvaI 141 CCTGG CC^[AT]GG
380 CCTGG
NdeII 231 GATC ^GATC
366 GATC
RsaI 162 GTAC GT^AC
189 GTAC
Sau3AI 231 GATC ^GATC
366 GATC
SfcI 192 CTACAG C^T[GA][CT]AG
205 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BssKI
Cfr13I Fnu4HI HaeIII ItaI MnlI
NlaIII Sau96I ScrFI TseI