################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccIII AclI AflII AhdI Aor51HI AscI Asp700I AspEI AviII AvrII BamHI BbvCI BciVI BfaI BfrI BlnI BsaBI BseAI BseRI BsiWI BsmBI Bsp1407I BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PmeI PshAI Psp1406I PvuI RcaI SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 2408 GGTACC G^GTACC AccI 3535 GTAGAC GT^[AC][GT]AC AocI 973 CCTGAGG CC^T[ACGT]AGG Asp718I 2408 GGTACC G^GTACC AspI 2050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 479 TGATCA T^GATCA BglII 885 AGATCT A^GATCT BsmI 2467 GAATGC GAATGC(1/-1) BsrDI 1290 CATTGC GCAATG(2/0) BstAPI 1670 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 973 CCTGAGG CC^T[ACGT]AGG CpoI 25 CGGTCCG CG^G[AT]CCG DraIII 2480 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 973 CCTGAGG CC^T[ACGT]AGG EcoNI 380 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 479 TGATCA T^GATCA HpaI 1177 GTTAAC GTT^AAC KpnI 2408 GGTACC GGTAC^C KspI 336 CCGCGG CCGC^GG NruI 2046 TCGCGA TCG^CGA PflFI 2050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1167 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsrII 25 CGGTCCG CG^G[AT]CCG SacI 3219 GAGCTC GAGCT^C SacII 336 CCGCGG CCGC^GG StuI 774 AGGCCT AGG^CCT Tth111I 2050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1167 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 1834 AAATTC [GA]^AATT[CT] 2952 GAATTT AgeI 2411 ACCGGT A^CCGGT 2525 ACCGGT Alw44I 195 GTGCAC G^TGCAC 2841 GTGCAC ApaLI 195 GTGCAC G^TGCAC 2841 GTGCAC ApoI 1834 AAATTC [GA]^AATT[CT] 2952 GAATTT BbeI 32 GGCGCC GGCGC^C 169 GGCGCC BglI 701 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 758 GCCAGTATGGC BlpI 1006 GCTGAGC GC^T[ACGT]AGC 3216 GCTGAGC BpmI 2540 CTCCAG CTGGAG(16/14) 2821 CTCCAG Bpu1102I 1006 GCTGAGC GC^T[ACGT]AGC 3216 GCTGAGC BsaWI 2411 ACCGGT [AT]^CCGG[AT] 2525 ACCGGT BsgI 1731 CTGCAC GTGCAG(16/14) 2787 CTGCAC BspLU11I 2272 ACATGT A^CATGT 3036 ACATGT CelII 1006 GCTGAGC GC^T[ACGT]AGC 3216 GCTGAGC EagI 21 CGGCCG C^GGCCG 260 CGGCCG EarI 3469 GAAGAG CTCTTC(1/4) 3544 GAAGAG EclXI 21 CGGCCG C^GGCCG 260 CGGCCG Eco52I 21 CGGCCG C^GGCCG 260 CGGCCG HaeII 32 GGCGCC [GA]GCGC^[CT] 169 GGCGCC KasI 32 GGCGCC G^GCGCC 169 GGCGCC Ksp632I 3469 GAAGAG CTCTTC(1/4) 3544 GAAGAG NaeI 491 GCCGGC GCC^GGC 3519 GCCGGC NarI 32 GGCGCC GG^CGCC 169 GGCGCC NcoI 1742 CCATGG C^CATGG 2594 CCATGG NgoMIV 491 GCCGGC G^CCGGC 3519 GCCGGC PinAI 2411 ACCGGT A^CCGGT 2525 ACCGGT SfoI 32 GGCGCC GGC^GCC 169 GGCGCC SmlI 235 CTCAAG C^T[CT][GA]AG 325 CTCAAG XcmI 2649 CCAGTGCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2781 CCACCCCTGCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcgI BcnI BmyI BpuAI BsaAI BsaHI BsaI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspDI BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI