(Circular) (Six-base) MAPSORT of: pFN21AE6739.seq Check: 8309 from: 1 to: 8359 pFN21AE6739 8359 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2748 3176 4169 2748 Size: 428 993 6938 Fragments arranged by size: 6938 993 428 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6169 278 Size: 53 83 186 936 4633 2468 Fragments arranged by size: 4633 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5541 5702 1937 Size: 3604 161 4594 Fragments arranged by size: 4594 3604 161 Acc65I G'GTAC_C Cuts at: 4413 5680 4413 Size: 1267 7092 AclI AA'CG_TT Cuts at: 1563 6486 6859 1563 Size: 4923 373 3063 Fragments arranged by size: 4923 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2534 3789 5007 5079 5210 5487 6422 7806 Size: 902 1255 1218 72 131 277 935 1384 Cuts at: 7806 8074 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1255 1218 935 902 277 268 131 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8162 AflIII A'CryG_T Cuts at: 1203 4046 4277 5041 7258 8235 1203 Size: 2843 231 764 2217 977 1327 Fragments arranged by size: 2843 2217 1327 977 764 231 AgeI A'CCGG_T Cuts at: 4416 4530 7190 4416 Size: 114 2660 5585 Fragments arranged by size: 5585 2660 114 AhdI GACnn_n'nnGTC Cuts at: 7088 7088 Size: 8359 AleI CACnn'nnGTG Cuts at: 1166 4116 4280 4394 4557 4799 1166 Size: 2950 164 114 163 242 4726 Fragments arranged by size: 4726 2950 242 164 163 114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2384 4575 1184 Size: 1200 2191 4968 Fragments arranged by size: 4968 2191 1200 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5417 5449 5417 Size: 32 8327 AlwNI CAG_nnn'CTG Cuts at: 2512 2954 3178 3538 4245 4660 5324 6102 7674 Size: 442 224 360 707 415 664 778 1572 Cuts at: 7674 2512 Size: 3197 Fragments arranged by size: 3197 1572 778 707 664 442 415 360 224 ApaI G_GGCC'C Cuts at: 2277 2684 4335 4449 2277 Size: 407 1651 114 6187 Fragments arranged by size: 6187 1651 407 114 ApaLI G'TGCA_C Cuts at: 2200 4846 6416 7572 2200 Size: 2646 1570 1156 2987 Fragments arranged by size: 2987 2646 1570 1156 ApoI r'AATT_y Cuts at: 1493 3839 4957 5668 5894 6046 8155 1493 Size: 2346 1118 711 226 152 2109 1697 Fragments arranged by size: 2346 2109 1697 1118 711 226 152 AseI AT'TA_AT Cuts at: 160 1032 3357 3894 4008 6913 160 Size: 872 2325 537 114 2905 1606 Fragments arranged by size: 2905 2325 1606 872 537 114 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8359 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2185 2476 2809 3659 4432 5684 Size: 73 155 87 291 333 850 773 1252 Cuts at: 5684 1870 Size: 4545 Fragments arranged by size: 4545 1252 850 773 333 291 155 87 73 BamHI G'GATC_C Cuts at: 5689 6056 5689 Size: 367 7992 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 2174 2760 3164 3614 Size: 325 217 627 250 137 586 404 450 Cuts at: 3614 4157 4388 4413 4559 5277 5680 7135 618 Size: 543 231 25 146 718 403 1455 1842 Fragments arranged by size: 1842 1455 718 627 586 543 450 404 403 325 250 231 217 146 137 25 BanII G_rGCy'C Cuts at: 1391 2277 2684 2841 4335 4449 5228 5678 1391 Size: 886 407 157 1494 114 779 450 4072 Fragments arranged by size: 4072 1494 886 779 450 407 157 114 BbeI G_GCGC'C Cuts at: 2041 2178 2041 Size: 137 8222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3311 4473 4809 4844 928 Size: 971 1412 1162 336 35 4443 Fragments arranged by size: 4443 1412 1162 971 336 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2344 2378 2697 2731 3458 Size: 34 50 34 323 34 319 34 727 Cuts at: 3458 3492 3919 3953 6550 6584 1903 Size: 34 427 34 2597 34 3678 Fragments arranged by size: 3678 2597 727 427 323 319 50 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6251 7461 6251 Size: 1210 7149 BclI T'GATC_A Cuts at: 1548 2484 1548 Size: 936 7423 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2712 2769 6970 Size: 107 122 71 631 605 1040 57 4201 Cuts at: 6970 136 Size: 1525 Fragments arranged by size: 4201 1525 1040 631 605 122 107 71 57 BglII A'GATC_T Cuts at: 2890 8354 2890 Size: 5464 2895 BlpI GC'TnA_GC Cuts at: 3012 5222 5776 3012 Size: 2210 554 5595 Fragments arranged by size: 5595 2210 554 Bme1580I G_kGCm'C Cuts at: 1790 2074 2204 2277 2684 3167 3206 3619 3659 Size: 284 130 73 407 483 39 413 40 Cuts at: 3659 4046 4277 4335 4432 4449 4554 4728 4739 Size: 387 231 58 97 17 105 174 11 Cuts at: 4739 4850 5629 6420 7576 1790 Size: 111 779 791 1156 2573 Fragments arranged by size: 2573 1156 791 779 483 413 407 387 284 231 174 130 111 105 97 73 58 40 39 17 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8359 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2875 3228 3613 Size: 416 53 83 399 24 1458 353 385 Cuts at: 3613 6090 7048 442 Size: 2477 958 1753 Fragments arranged by size: 2477 1753 1458 958 416 399 385 353 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8359 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5202 5202 Size: 8359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4530 4811 7019 1239 Size: 3291 281 2208 2579 Fragments arranged by size: 3291 2579 2208 281 Bpu10I CC'TnA_GC Cuts at: 2045 2290 2724 2045 Size: 245 434 7680 Fragments arranged by size: 7680 434 245 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 2315 6484 7349 7647 2225 Size: 90 4169 865 298 2937 Fragments arranged by size: 4169 2937 865 298 90 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2184 2852 3056 4579 7022 882 Size: 617 685 668 204 1523 2443 2219 Fragments arranged by size: 2443 2219 1523 685 668 617 204 BsaAI yAC'GTr Cuts at: 493 1204 4047 4118 4143 4305 6150 493 Size: 711 2843 71 25 162 1845 2702 Fragments arranged by size: 2843 2702 1845 711 162 71 25 BsaBI GATnn'nnATC Cuts at: 8353 8353 Size: 8359 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2038 2175 3387 6166 Size: 53 83 186 936 505 137 1212 2779 Cuts at: 6166 6548 7183 275 Size: 382 635 1451 Fragments arranged by size: 2779 1451 1212 936 635 505 382 186 137 83 53 BsaWI w'CCGG_w Cuts at: 4416 4530 6792 7190 7464 7611 8022 4416 Size: 114 2262 398 274 147 411 4753 Fragments arranged by size: 4753 2262 411 398 274 147 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 2237 2267 4898 Size: 30 165 30 705 30 99 30 2631 Cuts at: 4898 4928 5417 5447 1178 Size: 30 489 30 4090 Fragments arranged by size: 4090 2631 705 489 165 99 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8359 BseYI C'CCAG_C Cuts at: 2492 3978 4206 4323 4442 4739 7562 8058 2492 Size: 1486 228 117 119 297 2823 496 2793 Fragments arranged by size: 2823 2793 1486 496 297 228 119 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3721 4777 1163 Size: 573 1985 1056 4745 Fragments arranged by size: 4745 1985 1056 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2029 2268 2353 5809 6570 7598 Size: 275 186 28 239 85 3456 761 1028 Cuts at: 7598 1540 Size: 2301 Fragments arranged by size: 3456 2301 1028 761 275 239 186 85 28 BsiHKAI G_wGCw'C Cuts at: 2204 3018 4345 4850 4938 5228 6420 6505 7576 Size: 814 1327 505 88 290 1192 85 1071 Cuts at: 7576 2204 Size: 2987 Fragments arranged by size: 2987 1327 1192 1071 814 505 290 88 85 BsmI GAATG_Cn' Cuts at: 4478 5870 5963 4478 Size: 1392 93 6874 Fragments arranged by size: 6874 1392 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2204 2277 2684 2841 3018 3167 Size: 399 284 130 73 407 157 177 149 Cuts at: 3167 3206 3619 3659 4046 4277 4335 4345 4432 Size: 39 413 40 387 231 58 10 87 Cuts at: 4432 4449 4554 4728 4739 4850 4938 5228 5629 Size: 17 105 174 11 111 88 290 401 Cuts at: 5629 5678 6420 6505 7576 1391 Size: 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 413 407 401 399 387 290 284 231 177 174 157 149 130 111 105 88 87 85 73 58 49 40 39 17 11 10 BspEI T'CCGG_A Cuts at: 8022 8022 Size: 8359 BspHI T'CATG_A Cuts at: 6248 7978 6248 Size: 1730 6629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2748 3176 4115 4169 4658 5714 844 Size: 1904 428 939 54 489 1056 3489 Fragments arranged by size: 3489 1904 1056 939 489 428 54 BsrBI CCG'CTC Cuts at: 1394 1823 6246 1394 Size: 429 4423 3507 Fragments arranged by size: 4423 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3294 6854 7028 65 Size: 1183 2046 3560 174 1396 Fragments arranged by size: 3560 2046 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2496 3104 4416 4530 4831 5524 Size: 110 218 468 608 1312 114 301 693 Cuts at: 5524 7003 7190 1700 Size: 1479 187 2869 Fragments arranged by size: 2869 1479 1312 693 608 468 301 218 187 114 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8359 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8359 BssSI C'ACGA_G Cuts at: 6419 7431 6419 Size: 1012 7347 BstAPI GCAn_nnn'nTGC Cuts at: 794 3681 794 Size: 2887 5472 BstBI TT'CG_AA Cuts at: 7163 7163 Size: 8359 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8359 BstYI r'GATC_y Cuts at: 1972 2118 2550 2890 5247 5689 6056 6441 6458 Size: 146 432 340 2357 442 367 385 17 Cuts at: 6458 7910 7996 8008 8354 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2357 1977 1452 442 432 385 367 346 340 146 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2979 2979 Size: 8359 BtgI C'CryG_G Cuts at: 513 1065 2015 2341 2821 3157 3747 4599 513 Size: 552 950 326 480 336 590 852 4273 Fragments arranged by size: 4273 950 852 590 552 480 336 326 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4987 487 Size: 683 56 3761 3859 Fragments arranged by size: 3859 3761 683 56 BtsI GCAGTG_nn' Cuts at: 2507 2852 3700 4951 5875 6669 6689 2507 Size: 345 848 1251 924 794 20 4177 Fragments arranged by size: 4177 1251 924 848 794 345 20 ClaI AT'CG_AT Cuts at: 1554 3468 3582 3939 5112 1554 Size: 1914 114 357 1173 4801 Fragments arranged by size: 4801 1914 1173 357 114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2071 2106 2884 2919 3781 3816 4135 Size: 35 1507 35 778 35 862 35 319 Cuts at: 4135 4170 529 Size: 35 4718 Fragments arranged by size: 4718 1507 862 778 319 35 35 35 35 35 DraI TTT'AAA Cuts at: 5663 5663 Size: 8359 DraIII CAC_nnn'GTG Cuts at: 4490 4490 Size: 8359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7366 809 Size: 507 6050 1802 Fragments arranged by size: 6050 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2265 2387 Size: 54 1425 50 275 18 196 239 122 Cuts at: 2387 2872 3370 3744 5046 5806 6695 8324 8 Size: 485 498 374 1302 760 889 1629 43 Fragments arranged by size: 1629 1425 1302 889 760 498 485 374 275 239 196 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2026 2265 5806 1537 Size: 275 214 239 3541 4090 Fragments arranged by size: 4090 3541 275 239 214 EarI CTCTTCn'nnn_ Cuts at: 1353 5469 5544 6289 1353 Size: 4116 75 745 3423 Fragments arranged by size: 4116 3423 745 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5308 6946 7320 7466 387 Size: 1064 138 3719 1638 374 146 1280 Fragments arranged by size: 3719 1638 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5226 5226 Size: 8359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2534 3789 4530 4811 5007 5079 5210 Size: 393 902 1255 741 281 196 72 131 Cuts at: 5210 5487 6422 7019 7806 8074 1239 Size: 277 935 597 787 268 1524 Fragments arranged by size: 1524 1255 935 902 787 741 597 393 281 277 268 196 131 72 EcoNI CCTnn'n_nnAGG Cuts at: 2389 2389 Size: 8359 EcoO109I rG'GnC_Cy Cuts at: 2094 2273 2274 2394 2681 2951 2993 3175 3987 Size: 179 1 120 287 270 42 182 812 Cuts at: 3987 4331 2094 Size: 344 6122 Fragments arranged by size: 6122 812 344 287 270 182 179 120 42 1 EcoRI G'AATT_C Cuts at: 5668 6046 5668 Size: 378 7981 FspI TGC'GCA Cuts at: 6865 6865 Size: 8359 HaeII r_GCGC'y Cuts at: 1678 2041 2178 7506 1678 Size: 363 137 5328 2531 Fragments arranged by size: 5328 2531 363 137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2237 2269 4578 Size: 32 135 32 536 32 97 32 2309 Cuts at: 4578 4610 4799 4831 4898 4930 7000 7032 7074 Size: 32 189 32 67 32 2070 32 42 Cuts at: 7074 7106 1373 Size: 32 2626 Fragments arranged by size: 2626 2309 2070 536 189 135 97 67 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3184 3949 4288 5703 5949 669 Size: 988 281 1246 765 339 1415 246 3079 Fragments arranged by size: 3079 1415 1246 988 765 339 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8359 HpaI GTT'AAC Cuts at: 3184 5949 3184 Size: 2765 5594 KasI G'GCGC_C Cuts at: 2037 2174 2037 Size: 137 8222 KpnI G_GTAC'C Cuts at: 4417 5684 4417 Size: 1267 7092 MfeI C'AATT_G Cuts at: 5958 5958 Size: 8359 MluI A'CGCG_T Cuts at: 8235 8235 Size: 8359 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 3780 3816 7473 7657 1305 Size: 789 1686 36 3657 184 2007 Fragments arranged by size: 3657 2007 1686 789 184 36 MscI TGG'CCA Cuts at: 10 64 1489 1832 3372 3746 5048 10 Size: 54 1425 343 1540 374 1302 3321 Fragments arranged by size: 3321 1540 1425 1302 374 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4116 4280 4394 4557 4598 4799 6317 Size: 648 2950 164 114 163 41 201 1518 Cuts at: 6317 6676 6835 518 Size: 359 159 2042 Fragments arranged by size: 2950 2042 1518 648 359 201 164 163 159 114 41 MspA1I CmG'CkG Cuts at: 1606 2343 3054 3574 3735 4386 4743 4985 5359 Size: 737 711 520 161 651 357 242 374 Cuts at: 5359 5775 6452 7600 7845 1606 Size: 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 711 677 651 520 416 374 357 245 242 161 NaeI GCC'GGC Cuts at: 2498 5526 2498 Size: 3028 5331 NarI GG'CG_CC Cuts at: 2038 2175 2038 Size: 137 8222 NcoI C'CATG_G Cuts at: 513 1065 3747 4599 513 Size: 552 2682 852 4273 Fragments arranged by size: 4273 2682 852 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8359 NgoMIV G'CCGG_C Cuts at: 2496 5524 2496 Size: 3028 5331 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8359 NotI GC'GGCC_GC Cuts at: 5806 5806 Size: 8359 NruI TCG'CGA Cuts at: 4053 4053 Size: 8359 NspI r_CATG'y Cuts at: 1132 1229 3117 4195 4281 5045 5717 7262 1132 Size: 97 1888 1078 86 764 672 1545 2229 Fragments arranged by size: 2229 1888 1545 1078 764 672 97 86 PasI CC'CwG_GG Cuts at: 1348 3070 1348 Size: 1722 6637 PciI A'CATG_T Cuts at: 4277 5041 7258 4277 Size: 764 2217 5378 Fragments arranged by size: 5378 2217 764 PflMI CCAn_nnn'nTGG Cuts at: 1108 3178 1108 Size: 2070 6289 PfoI T'CCnGG_A Cuts at: 4764 4764 Size: 8359 PmeI GTTT'AAAC Cuts at: 5663 5663 Size: 8359 PmlI CAC'GTG Cuts at: 4047 4118 4143 4047 Size: 71 25 8263 Fragments arranged by size: 8263 71 25 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5417 5449 6428 6460 7941 7973 5417 Size: 32 979 32 1481 32 5803 Fragments arranged by size: 5803 1481 979 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2394 2993 3175 2394 Size: 599 182 7578 Fragments arranged by size: 7578 599 182 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8359 PsiI TTA'TAA Cuts at: 5929 5929 Size: 8359 PspOMI G'GGCC_C Cuts at: 2273 2680 4331 4445 2273 Size: 407 1651 114 6187 Fragments arranged by size: 6187 1651 407 114 PstI C_TGCA'G Cuts at: 830 2154 2282 2720 2759 4019 4364 4457 5711 Size: 1324 128 438 39 1260 345 93 1254 Cuts at: 5711 830 Size: 3478 Fragments arranged by size: 3478 1324 1260 1254 438 345 128 93 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8359 PvuII CAG'CTG Cuts at: 3054 3574 3735 5359 3054 Size: 520 161 1624 6054 Fragments arranged by size: 6054 1624 520 161 RsrII CG'GwC_CG Cuts at: 2031 2031 Size: 8359 SacI G_AGCT'C Cuts at: 5228 5228 Size: 8359 SacII CC_GC'GG Cuts at: 2344 2344 Size: 8359 SalI G'TCGA_C Cuts at: 1936 5701 1936 Size: 3765 4594 SbfI CC_TGCA'GG Cuts at: 5711 5711 Size: 8359 ScaI AGT'ACT Cuts at: 6607 8319 6607 Size: 1712 6647 SexAI A'CCwGG_T Cuts at: 3177 4056 4918 3177 Size: 879 862 6618 Fragments arranged by size: 6618 879 862 SfcI C'TryA_G Cuts at: 826 1045 2150 2278 2716 2755 3967 4015 4360 Size: 219 1105 128 438 39 1212 48 345 Cuts at: 4360 4453 5707 6842 7523 7714 826 Size: 93 1254 1135 681 191 1471 Fragments arranged by size: 1471 1254 1212 1135 1105 681 438 345 219 191 128 93 48 39 SfoI GGC'GCC Cuts at: 2039 2176 2039 Size: 137 8222 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8359 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8359 SmaI CCC'GGG Cuts at: 1872 2187 2811 4434 5686 1872 Size: 315 624 1623 1252 4545 Fragments arranged by size: 4545 1623 1252 624 315 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 2330 6463 7364 7626 820 Size: 197 926 297 90 4133 901 262 1553 Fragments arranged by size: 4133 1553 926 901 297 262 197 90 SnaBI TAC'GTA Cuts at: 493 6150 493 Size: 5657 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8359 SphI G_CATG'C Cuts at: 1132 5717 1132 Size: 4585 3774 SspI AAT'ATT Cuts at: 5 52 3355 3892 4006 4966 4991 6283 5 Size: 47 3303 537 114 960 25 1292 2081 Fragments arranged by size: 3303 2081 1292 960 537 114 47 25 StuI AGG'CCT Cuts at: 1517 2781 1517 Size: 1264 7095 StyI C'CwwG_G Cuts at: 513 1065 2082 3367 3747 4599 5798 513 Size: 552 1017 1285 380 852 1199 3074 Fragments arranged by size: 3074 1285 1199 1017 852 552 380 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4080 4803 5598 6386 6403 6556 6741 Size: 1255 2500 723 795 788 17 153 185 Cuts at: 6741 7186 325 Size: 445 1498 Fragments arranged by size: 2500 1498 1255 795 788 723 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 5649 6605 Size: 274 80 33 51 1443 807 2865 956 Cuts at: 6605 8317 96 Size: 1712 138 Fragments arranged by size: 2865 1712 1443 956 807 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4760 4792 8319 8351 4760 Size: 32 3527 32 4768 Fragments arranged by size: 4768 3527 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4058 1316 Size: 2742 5617 XbaI T'CTAG_A Cuts at: 5695 5695 Size: 8359 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4661 4793 4661 Size: 132 8227 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8359 XmaI C'CCGG_G Cuts at: 1870 2185 2809 4432 5684 1870 Size: 315 624 1623 1252 4545 Fragments arranged by size: 4545 1623 1252 624 315 XmnI GAAnn'nnTTC Cuts at: 6488 6488 Size: 8359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6167 276 Size: 53 83 186 936 4633 2468 Fragments arranged by size: 4633 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI NsiI PacI PspXI PsrI SanDI SapI SfiI SrfI SwaI