(Linear) (Six-base) MAPSORT of: KIEE6739.seq Check: 2271 from: 1 to: 3654 KIEE6739.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 743 1171 2164 3654 Size: 743 428 993 1490 Fragments arranged by size: 1490 993 743 428 AccI GT'mk_AC Cuts at: 0 3536 3654 Size: 3536 118 Acc65I G'GTAC_C Cuts at: 0 2408 3654 Size: 2408 1246 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 1784 3002 3074 3205 3482 3654 Size: 529 1255 1218 72 131 277 172 Fragments arranged by size: 1255 1218 529 277 172 131 72 AflIII A'CryG_T Cuts at: 0 2041 2272 3036 3654 Size: 2041 231 764 618 Fragments arranged by size: 2041 764 618 231 AgeI A'CCGG_T Cuts at: 0 2411 2525 3654 Size: 2411 114 1129 Fragments arranged by size: 2411 1129 114 AleI CACnn'nnGTG Cuts at: 0 2111 2275 2389 2552 2794 3654 Size: 2111 164 114 163 242 860 Fragments arranged by size: 2111 860 242 164 163 114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 379 2570 3654 Size: 379 2191 1084 Fragments arranged by size: 2191 1084 379 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3412 3444 3654 Size: 3412 32 210 Fragments arranged by size: 3412 210 32 AlwNI CAG_nnn'CTG Cuts at: 0 507 949 1173 1533 2240 2655 3319 3654 Size: 507 442 224 360 707 415 664 335 Fragments arranged by size: 707 664 507 442 415 360 335 224 ApaI G_GGCC'C Cuts at: 0 272 679 2330 2444 3654 Size: 272 407 1651 114 1210 Fragments arranged by size: 1651 1210 407 272 114 ApaLI G'TGCA_C Cuts at: 0 195 2841 3654 Size: 195 2646 813 Fragments arranged by size: 2646 813 195 ApoI r'AATT_y Cuts at: 0 1834 2952 3654 Size: 1834 1118 702 Fragments arranged by size: 1834 1118 702 AseI AT'TA_AT Cuts at: 0 1352 1889 2003 3654 Size: 1352 537 114 1651 Fragments arranged by size: 1651 1352 537 114 AvaI C'yCGr_G Cuts at: 0 93 180 471 804 1654 2427 3654 Size: 93 87 291 333 850 773 1227 Fragments arranged by size: 1227 850 773 333 291 93 87 BanI G'GyrC_C Cuts at: 0 32 169 755 1159 1609 2152 2383 2408 Size: 32 137 586 404 450 543 231 25 Cuts at: 2408 2554 3272 3654 Size: 146 718 382 Fragments arranged by size: 718 586 543 450 404 382 231 146 137 32 25 BanII G_rGCy'C Cuts at: 0 272 679 836 2330 2444 3223 3654 Size: 272 407 157 1494 114 779 431 Fragments arranged by size: 1494 779 431 407 272 157 114 BbeI G_GCGC'C Cuts at: 0 36 173 3654 Size: 36 137 3481 Fragments arranged by size: 3481 137 36 BbsI GAAGACnn'nnnn_ Cuts at: 0 1306 2468 2804 2839 3654 Size: 1306 1162 336 35 815 Fragments arranged by size: 1306 1162 815 336 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 339 373 692 726 1453 1487 1914 1948 Size: 339 34 319 34 727 34 427 34 Cuts at: 1948 3654 Size: 1706 Fragments arranged by size: 1706 727 427 339 319 34 34 34 34 BclI T'GATC_A Cuts at: 0 479 3654 Size: 479 3175 BglI GCCn_nnn'nGGC Cuts at: 0 707 764 3654 Size: 707 57 2890 Fragments arranged by size: 2890 707 57 BglII A'GATC_T Cuts at: 0 885 3654 Size: 885 2769 BlpI GC'TnA_GC Cuts at: 0 1007 3217 3654 Size: 1007 2210 437 Fragments arranged by size: 2210 1007 437 Bme1580I G_kGCm'C Cuts at: 0 69 199 272 679 1162 1201 1614 1654 Size: 69 130 73 407 483 39 413 40 Cuts at: 1654 2041 2272 2330 2427 2444 2549 2723 2734 Size: 387 231 58 97 17 105 174 11 Cuts at: 2734 2845 3624 3654 Size: 111 779 30 Fragments arranged by size: 779 483 413 407 387 231 174 130 111 105 97 73 69 58 40 39 30 17 11 BmrI ACTGGGnnnn_n' Cuts at: 0 870 1223 1608 3654 Size: 870 353 385 2046 Fragments arranged by size: 2046 870 385 353 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3197 3654 Size: 3197 457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2525 2806 3654 Size: 2525 281 848 Fragments arranged by size: 2525 848 281 Bpu10I CC'TnA_GC Cuts at: 0 40 285 719 3654 Size: 40 245 434 2935 Fragments arranged by size: 2935 434 245 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 310 3654 Size: 220 90 3344 Fragments arranged by size: 3344 220 90 BsaI GGTCTCn'nnnn_ Cuts at: 0 179 847 1051 2574 3654 Size: 179 668 204 1523 1080 Fragments arranged by size: 1523 1080 668 204 179 BsaAI yAC'GTr Cuts at: 0 2042 2113 2138 2300 3654 Size: 2042 71 25 162 1354 Fragments arranged by size: 2042 1354 162 71 25 BsaHI Gr'CG_yC Cuts at: 0 33 170 1382 3654 Size: 33 137 1212 2272 Fragments arranged by size: 2272 1212 137 33 BsaWI w'CCGG_w Cuts at: 0 2411 2525 3654 Size: 2411 114 1129 Fragments arranged by size: 2411 1129 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 103 133 232 262 2893 2923 3412 3442 Size: 103 30 99 30 2631 30 489 30 Cuts at: 3442 3654 Size: 212 Fragments arranged by size: 2631 489 212 103 99 30 30 30 30 BseYI C'CCAG_C Cuts at: 0 487 1973 2201 2318 2437 2734 3654 Size: 487 1486 228 117 119 297 920 Fragments arranged by size: 1486 920 487 297 228 119 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1716 2772 3654 Size: 1716 1056 882 Fragments arranged by size: 1716 1056 882 BsiEI CG_ry'CG Cuts at: 0 24 263 348 3654 Size: 24 239 85 3306 Fragments arranged by size: 3306 239 85 24 BsiHKAI G_wGCw'C Cuts at: 0 199 1013 2340 2845 2933 3223 3654 Size: 199 814 1327 505 88 290 431 Fragments arranged by size: 1327 814 505 431 290 199 88 BsmI GAATG_Cn' Cuts at: 0 2473 3654 Size: 2473 1181 Bsp1286I G_dGCh'C Cuts at: 0 69 199 272 679 836 1013 1162 1201 Size: 69 130 73 407 157 177 149 39 Cuts at: 1201 1614 1654 2041 2272 2330 2340 2427 2444 Size: 413 40 387 231 58 10 87 17 Cuts at: 2444 2549 2723 2734 2845 2933 3223 3624 3654 Size: 105 174 11 111 88 290 401 30 Fragments arranged by size: 413 407 401 387 290 231 177 174 157 149 130 111 105 88 87 73 69 58 40 39 30 17 11 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 743 1171 2110 2164 2653 3654 Size: 743 428 939 54 489 1001 Fragments arranged by size: 1001 939 743 489 428 54 BsrDI GCAATG_nn' Cuts at: 0 1289 3654 Size: 1289 2365 BsrFI r'CCGG_y Cuts at: 0 23 491 1099 2411 2525 2826 3519 3654 Size: 23 468 608 1312 114 301 693 135 Fragments arranged by size: 1312 693 608 468 301 135 114 23 BstAPI GCAn_nnn'nTGC Cuts at: 0 1676 3654 Size: 1676 1978 BstYI r'GATC_y Cuts at: 0 113 545 885 3242 3654 Size: 113 432 340 2357 412 Fragments arranged by size: 2357 432 412 340 113 Bsu36I CC'TnA_GG Cuts at: 0 974 3654 Size: 974 2680 BtgI C'CryG_G Cuts at: 0 10 336 816 1152 1742 2594 3654 Size: 10 326 480 336 590 852 1060 Fragments arranged by size: 1060 852 590 480 336 326 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2982 3654 Size: 2982 672 BtsI GCAGTG_nn' Cuts at: 0 502 847 1695 2946 3654 Size: 502 345 848 1251 708 Fragments arranged by size: 1251 848 708 502 345 ClaI AT'CG_AT Cuts at: 0 1463 1577 1934 3107 3654 Size: 1463 114 357 1173 547 Fragments arranged by size: 1463 1173 547 357 114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 66 101 879 914 1776 1811 2130 2165 Size: 66 35 778 35 862 35 319 35 Cuts at: 2165 3654 Size: 1489 Fragments arranged by size: 1489 862 778 319 66 35 35 35 35 DraIII CAC_nnn'GTG Cuts at: 0 2485 3654 Size: 2485 1169 EaeI y'GGCC_r Cuts at: 0 21 260 382 867 1365 1739 3041 3654 Size: 21 239 122 485 498 374 1302 613 Fragments arranged by size: 1302 613 498 485 374 239 122 21 EagI C'GGCC_G Cuts at: 0 21 260 3654 Size: 21 239 3394 Fragments arranged by size: 3394 239 21 EarI CTCTTCn'nnn_ Cuts at: 0 3464 3539 3654 Size: 3464 75 115 Fragments arranged by size: 3464 115 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3303 3654 Size: 3303 351 EcoICRI GAG'CTC Cuts at: 0 3221 3654 Size: 3221 433 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 1784 2525 2806 3002 3074 3205 3482 Size: 529 1255 741 281 196 72 131 277 Cuts at: 3482 3654 Size: 172 Fragments arranged by size: 1255 741 529 281 277 196 172 131 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 384 3654 Size: 384 3270 EcoO109I rG'GnC_Cy Cuts at: 0 89 268 269 389 676 946 988 1170 Size: 89 179 1 120 287 270 42 182 Cuts at: 1170 1982 2326 3654 Size: 812 344 1328 Fragments arranged by size: 1328 812 344 287 270 182 179 120 89 42 1 HaeII r_GCGC'y Cuts at: 0 36 173 3654 Size: 36 137 3481 Fragments arranged by size: 3481 137 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 103 135 232 264 2573 2605 2794 2826 Size: 103 32 97 32 2309 32 189 32 Cuts at: 2826 2893 2925 3654 Size: 67 32 729 Fragments arranged by size: 2309 729 189 103 97 67 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1179 1944 2283 3654 Size: 1179 765 339 1371 Fragments arranged by size: 1371 1179 765 339 HpaI GTT'AAC Cuts at: 0 1179 3654 Size: 1179 2475 KasI G'GCGC_C Cuts at: 0 32 169 3654 Size: 32 137 3485 Fragments arranged by size: 3485 137 32 KpnI G_GTAC'C Cuts at: 0 2412 3654 Size: 2412 1242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 89 1775 1811 3654 Size: 89 1686 36 1843 Fragments arranged by size: 1843 1686 89 36 MscI TGG'CCA Cuts at: 0 1367 1741 3043 3654 Size: 1367 374 1302 611 Fragments arranged by size: 1367 1302 611 374 MslI CAynn'nnrTG Cuts at: 0 2111 2275 2389 2552 2593 2794 3654 Size: 2111 164 114 163 41 201 860 Fragments arranged by size: 2111 860 201 164 163 114 41 MspA1I CmG'CkG Cuts at: 0 338 1049 1569 1730 2381 2738 2980 3354 Size: 338 711 520 161 651 357 242 374 Cuts at: 3354 3654 Size: 300 Fragments arranged by size: 711 651 520 374 357 338 300 242 161 NaeI GCC'GGC Cuts at: 0 493 3521 3654 Size: 493 3028 133 Fragments arranged by size: 3028 493 133 NarI GG'CG_CC Cuts at: 0 33 170 3654 Size: 33 137 3484 Fragments arranged by size: 3484 137 33 NcoI C'CATG_G Cuts at: 0 1742 2594 3654 Size: 1742 852 1060 Fragments arranged by size: 1742 1060 852 NgoMIV G'CCGG_C Cuts at: 0 491 3519 3654 Size: 491 3028 135 Fragments arranged by size: 3028 491 135 NruI TCG'CGA Cuts at: 0 2048 3654 Size: 2048 1606 NspI r_CATG'y Cuts at: 0 1112 2190 2276 3040 3654 Size: 1112 1078 86 764 614 Fragments arranged by size: 1112 1078 764 614 86 PasI CC'CwG_GG Cuts at: 0 1065 3654 Size: 1065 2589 PciI A'CATG_T Cuts at: 0 2272 3036 3654 Size: 2272 764 618 Fragments arranged by size: 2272 764 618 PflMI CCAn_nnn'nTGG Cuts at: 0 1173 3654 Size: 1173 2481 PfoI T'CCnGG_A Cuts at: 0 2759 3654 Size: 2759 895 PmlI CAC'GTG Cuts at: 0 2042 2113 2138 3654 Size: 2042 71 25 1516 Fragments arranged by size: 2042 1516 71 25 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3412 3444 3654 Size: 3412 32 210 Fragments arranged by size: 3412 210 32 PpuMI rG'GwC_Cy Cuts at: 0 389 988 1170 3654 Size: 389 599 182 2484 Fragments arranged by size: 2484 599 389 182 PspOMI G'GGCC_C Cuts at: 0 268 675 2326 2440 3654 Size: 268 407 1651 114 1214 Fragments arranged by size: 1651 1214 407 268 114 PstI C_TGCA'G Cuts at: 0 149 277 715 754 2014 2359 2452 3654 Size: 149 128 438 39 1260 345 93 1202 Fragments arranged by size: 1260 1202 438 345 149 128 93 39 PvuII CAG'CTG Cuts at: 0 1049 1569 1730 3354 3654 Size: 1049 520 161 1624 300 Fragments arranged by size: 1624 1049 520 300 161 RsrII CG'GwC_CG Cuts at: 0 26 3654 Size: 26 3628 SacI G_AGCT'C Cuts at: 0 3223 3654 Size: 3223 431 SacII CC_GC'GG Cuts at: 0 339 3654 Size: 339 3315 SexAI A'CCwGG_T Cuts at: 0 1172 2051 2913 3654 Size: 1172 879 862 741 Fragments arranged by size: 1172 879 862 741 SfcI C'TryA_G Cuts at: 0 145 273 711 750 1962 2010 2355 2448 Size: 145 128 438 39 1212 48 345 93 Cuts at: 2448 3654 Size: 1206 Fragments arranged by size: 1212 1206 438 345 145 128 93 48 39 SfoI GGC'GCC Cuts at: 0 34 171 3654 Size: 34 137 3483 Fragments arranged by size: 3483 137 34 SmaI CCC'GGG Cuts at: 0 182 806 2429 3654 Size: 182 624 1623 1225 Fragments arranged by size: 1623 1225 624 182 SmlI C'TyrA_G Cuts at: 0 235 325 3654 Size: 235 90 3329 Fragments arranged by size: 3329 235 90 SspI AAT'ATT Cuts at: 0 1350 1887 2001 2961 2986 3654 Size: 1350 537 114 960 25 668 Fragments arranged by size: 1350 960 668 537 114 25 StuI AGG'CCT Cuts at: 0 776 3654 Size: 776 2878 StyI C'CwwG_G Cuts at: 0 77 1362 1742 2594 3654 Size: 77 1285 380 852 1060 Fragments arranged by size: 1285 1060 852 380 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2075 2798 3593 3654 Size: 2075 723 795 61 Fragments arranged by size: 2075 795 723 61 TatI w'GTAC_w Cuts at: 0 779 3644 3654 Size: 779 2865 10 Fragments arranged by size: 2865 779 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2755 2787 3654 Size: 2755 32 867 Fragments arranged by size: 2755 867 32 Tth111I GACn'n_nGTC Cuts at: 0 2053 3654 Size: 2053 1601 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2656 2788 3654 Size: 2656 132 866 Fragments arranged by size: 2656 866 132 XmaI C'CCGG_G Cuts at: 0 180 804 2427 3654 Size: 180 624 1623 1227 Fragments arranged by size: 1623 1227 624 180 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI BcgI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII SacI SacII SexAI SfcI SfoI SmaI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AclI AfeI AflII AhdI AscI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BmgBI BmtI BsaBI BseRI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII MfeI MluI NdeI NheI NotI NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI XbaI XhoI XmnI ZraI