Restriction map for
pFN21AB7041
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BamHI BbrPI
BciVI BclI BfrI BglII BsaBI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I EarI Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MfeI MluI MseI MunI NdeI
NheI NruI NsiI NspV PacI
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1297 GTCTAC GT^[AC][GT]AC
AccIII 1508 TCCGGA T^CCGGA
AgeI 578 ACCGGT A^CCGGT
AlwI 1560 GATCC GGATC(4/5)
AlwNI 555 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 1629 CCTAGG C^CTAGG
BalI 1049 TGGCCA TGG^CCA
BbvCI 552 CCTCAGC CCTCAGC(-5/-2)
BcgI 1520 CGACCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1630 CTAG C^TAG
BlnI 1629 CCTAGG C^CTAGG
BlpI 432 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 432 GCTGAGC GC^T[ACGT]AGC
BseAI 1508 TCCGGA T^CCGGA
BsmBI 1243 CGTCTC CGTCTC(1/5)
BspEI 1508 TCCGGA T^CCGGA
BspHI 620 TCATGA T^CATGA
BssSI 1642 CACGAG CACGAG(-5/-1)
BstAPI 954 GCACGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 337 GGTAACC G^GT[ACGT]ACC
BstPI 337 GGTAACC G^GT[ACGT]ACC
BstYI 1559 AGATCC [GA]^GATC[CT]
CelII 432 GCTGAGC GC^T[ACGT]AGC
DraIII 1642 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1332 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 337 GGTAACC G^GT[ACGT]ACC
HinfI 1044 GACTC G^A[ACGT]TC
KspI 203 CCGCGG CCGC^GG
MaeI 1630 CTAG C^TAG
MflI 1559 AGATCC [GA]^GATC[CT]
MluNI 1049 TGGCCA TGG^CCA
MroI 1508 TCCGGA T^CCGGA
MscI 1049 TGGCCA TGG^CCA
NaeI 1271 GCCGGC GCC^GGC
NgoMIV 1271 GCCGGC G^CCGGC
NotI 637 GCGGCCGC GC^GGCCGC
NspI 959 GCATGC [GA]CATG^[CT]
PaeR7I 862 CTCGAG C^TCGAG
PinAI 578 ACCGGT A^CCGGT
PleI 1044 GACTC GAGTC(4/5)
PpuMI 36 GGGACCC [GA]G^G[AT]CC[CT]
PstI 379 CTGCAG CTGCA^G
RcaI 620 TCATGA T^CATGA
SacI 198 GAGCTC GAGCT^C
SacII 203 CCGCGG CCGC^GG
SgrAI 1270 CGCCGGCG C[GA]^CCGG[CT]G
SphI 959 GCATGC GCATG^C
Tsp45I 388 GTCAC ^GT[GC]AC
Tsp509I 1679 AATT ^AATT
XcmI 464 CCATGGAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 862 CTCGAG C^TCGAG
XhoII 1559 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Aor51HI 728 AGCGCT AGC^GCT
1604 AGCGCT
ApaI 117 GGGCCC GGGCC^C
688 GGGCCC
AvaII 37 GGACC G^G[AT]CC
576 GGACC
AviII 911 TGCGCA TGC^GCA
962 TGCGCA
BglI 643 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
943 GCCGACGGGGC
BpmI 66 CTCCAG CTGGAG(16/14)
1542 CTCCAG
BsaAI 583 TACGTG [CT]AC^GT[GA]
832 TACGTG
BsaI 1512 GAGACC GGTCTC(1/5)
1705 GAGACC
BseRI 771 CTCCTC GAGGAG(10/8)
748 GAGGAG
BsgI 537 CTGCAC GTGCAG(16/14)
561 CTGCAC
BsrBI 1105 CCGCTC CCGCTC(-3/-3)
1195 CCGCTC
BsrFI 578 ACCGGT [GA]^CCGG[CT]
1271 GCCGGC
Cfr10I 578 ACCGGT [GA]^CCGG[CT]
1271 GCCGGC
DpnII 13 GATC ^GATC
1560 GATC
EagI 254 CGGCCG C^GGCCG
638 CGGCCG
EclXI 254 CGGCCG C^GGCCG
638 CGGCCG
Eco47III 728 AGCGCT AGC^GCT
1604 AGCGCT
Eco52I 254 CGGCCG C^GGCCG
638 CGGCCG
FspI 911 TGCGCA TGC^GCA
962 TGCGCA
MaeIII 338 GTAAC ^GT[ACGT]AC
388 GTCAC
MboI 13 GATC ^GATC
1560 GATC
NcoI 464 CCATGG C^CATGG
797 CCATGG
NdeII 13 GATC ^GATC
1560 GATC
Sau3AI 13 GATC ^GATC
1560 GATC
SfaNI 1437 GATGC GCATC(5/9)
914 GCATC
SfcI 379 CTGCAG C^T[GA][CT]AG
1689 CTACAG
StuI 893 AGGCCT AGG^CCT
1670 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanI BanII BbeI
BbsI BbvI BcnI BmyI BpuAI
BsaHI BsaJI BsaWI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NlaIII
NlaIV PvuII RsaI Sau96I ScrFI
SfoI SmlI StyI TaqI TseI
TspRI TthHB8I