Restriction map for
pFN21AB7041
(Circular) (Six-base) MAPSORT of: pFN21AB7041.seq Check: 6872 from: 1 to: 6421
pFN21AB7041 6421 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 3677 2391
Size: 1286 5135
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4231 278
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3303 3764 1937
Size: 1366 461 4594
Fragments arranged by size:
4594 1366 461
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3155 4484 5868 6136 1632
Size: 1523 1329 1384 268 1917
Fragments arranged by size:
1917 1523 1384 1329 268
AfeI AGC'GCT
Cuts at: 2735 3611 2735
Size: 876 5545
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 5320 6297 1203
Size: 4117 977 1327
Fragments arranged by size:
4117 1327 977
AgeI A'CCGG_T
Cuts at: 2583 5252 2583
Size: 2669 3752
AhdI GACnn_n'nnGTC
Cuts at: 5150 5150
Size: 6421
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 2565 4164 5736 2565
Size: 1599 1572 3250
Fragments arranged by size:
3250 1599 1572
ApaI G_GGCC'C
Cuts at: 2126 2697 2126
Size: 571 5850
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 3730 3956 4108 6217 1493
Size: 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 3355 3437 3746 1870
Size: 73 924 488 82 309 4545
Fragments arranged by size:
4545 924 488 309 82 73
AvrII C'CTAG_G
Cuts at: 3634 3634
Size: 6421
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2597 2630 2597
Size: 33 6388
BamHI G'GATC_C
Cuts at: 3751 4118 3751
Size: 367 6054
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2945 3279 3662 3742 5197
Size: 325 217 627 1158 334 383 80 1455
Cuts at: 5197 618
Size: 1842
Fragments arranged by size:
1842 1455 1158 627 383 334 325 217
80
BanII G_rGCy'C
Cuts at: 1391 2072 2126 2207 2697 2718 3740 1391
Size: 681 54 81 490 21 1022 4072
Fragments arranged by size:
4072 1022 681 490 81 54 21
BbeI G_GCGC'C
Cuts at: 2949 3283 3666 2949
Size: 334 383 5704
Fragments arranged by size:
5704 383 334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 2764 3064 3145 928
Size: 971 400 465 300 81 4204
Fragments arranged by size:
4204 971 465 400 300 81
BbvCI CC'TCA_GC
Cuts at: 2558 2558
Size: 6421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3514 3548 4612 4646 1903
Size: 34 1577 34 1064 34 3678
Fragments arranged by size:
3678 1577 1064 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4313 5523 4313
Size: 1210 5211
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6421
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2654 2954 5032
Size: 107 122 71 631 605 982 300 2078
Cuts at: 5032 136
Size: 1525
Fragments arranged by size:
2078 1525 982 631 605 300 122 107
71
BglII A'GATC_T
Cuts at: 6416 6416
Size: 6421
BlpI GC'TnA_GC
Cuts at: 2438 3838 2438
Size: 1400 5021
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2697 2724 4482 5638 1790
Size: 336 571 27 1758 1156 2573
Fragments arranged by size:
2573 1758 1156 571 336 27
BmgBI CAC'GTC
Cuts at: 1318 2245 2533 1318
Size: 927 288 5206
Fragments arranged by size:
5206 927 288
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4152 5110 442
Size: 416 53 83 399 24 2735 958 1753
Fragments arranged by size:
2735 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2056 3532 5081 1239
Size: 817 1476 1549 2579
Fragments arranged by size:
2579 1549 1476 817
Bpu10I CC'TnA_GC
Cuts at: 2024 2558 2024
Size: 534 5887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 3002 4546 5411 5709 2594
Size: 408 1544 865 298 3306
Fragments arranged by size:
3306 1544 865 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3511 3704 5084 882
Size: 617 2012 193 1380 2219
Fragments arranged by size:
2219 2012 1380 617 193
BsaAI yAC'GTr
Cuts at: 493 1204 2590 2839 4212 493
Size: 711 1386 249 1373 2702
Fragments arranged by size:
2702 1386 1373 711 249
BsaBI GATnn'nnATC
Cuts at: 6415 6415
Size: 6421
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 2877 2946 3150
Size: 53 83 186 936 771 573 69 204
Cuts at: 3150 3280 3639 3663 4228 4610 5245 275
Size: 130 359 24 565 382 635 1451
Fragments arranged by size:
1451 936 771 635 573 565 382 359
204 186 130 83 69 53 24
BsaWI w'CCGG_w
Cuts at: 2199 2583 3451 3513 4854 5252 5526 5673 6084
Size: 384 868 62 1341 398 274 147 411
Cuts at: 6084 2199
Size: 2536
Fragments arranged by size:
2536 1341 868 411 398 384 274 147
62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 3614 3644 1178
Size: 30 165 30 786 30 1395 30 3955
Fragments arranged by size:
3955 1395 786 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2767 2768 1186
Size: 1581 1 4839
Fragments arranged by size:
4839 1581 1
BseYI C'CCAG_C
Cuts at: 2617 2701 3476 3502 3528 3606 5624 6120 2617
Size: 84 775 26 26 78 2018 496 2918
Fragments arranged by size:
2918 2018 775 496 84 78 26 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2527 2551 1163
Size: 573 791 24 5033
Fragments arranged by size:
5033 791 573 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2262 2646 3456 3871 4632 5660
Size: 275 186 261 384 810 415 761 1028
Cuts at: 5660 1540
Size: 2301
Fragments arranged by size:
2301 1028 810 761 415 384 275 261
186
BsiHKAI G_wGCw'C
Cuts at: 2207 2789 3081 4482 4567 5638 2207
Size: 582 292 1401 85 1071 2990
Fragments arranged by size:
2990 1401 1071 582 292 85
BsmI GAATG_Cn'
Cuts at: 3932 4025 3932
Size: 93 6328
BsmBI CGTCTCn'nnnn_
Cuts at: 3254 3254
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2126 2207 2697 2718 2724 2789
Size: 399 282 54 81 490 21 6 65
Cuts at: 2789 3081 3740 4482 4567 5638 1391
Size: 292 659 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 659 490 399 292 282
85 81 65 54 21 6
BspEI T'CCGG_A
Cuts at: 3513 6084 3513
Size: 2571 3850
BspHI T'CATG_A
Cuts at: 2625 4310 6040 2625
Size: 1685 1730 3006
Fragments arranged by size:
3006 1730 1685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2330 2355 2391 2439 2805 3677 3776 844
Size: 1486 25 36 48 366 872 99 3489
Fragments arranged by size:
3489 1486 872 366 99 48 36 25
BsrBI CCG'CTC
Cuts at: 1394 1823 3112 3202 4308 1394
Size: 429 1289 90 1106 3507
Fragments arranged by size:
3507 1289 1106 429 90
BsrDI GCAATG_nn'
Cuts at: 65 1248 4916 5090 65
Size: 1183 3668 174 1396
Fragments arranged by size:
3668 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2583 3276 5065 5252 1700
Size: 110 773 693 1789 187 2869
Fragments arranged by size:
2869 1789 773 693 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6421
BssHII G'CGCG_C
Cuts at: 1925 2283 2846 3235 3535 3579 3586 1925
Size: 358 563 389 300 44 7 4760
Fragments arranged by size:
4760 563 389 358 300 44 7
BssSI C'ACGA_G
Cuts at: 3647 4481 5493 3647
Size: 834 1012 4575
Fragments arranged by size:
4575 1012 834
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2965 794
Size: 2171 4250
BstBI TT'CG_AA
Cuts at: 5225 5225
Size: 6421
BstEII G'GTnAC_C
Cuts at: 1184 2342 1184
Size: 1158 5263
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 3052 3064 2578
Size: 474 12 5935
Fragments arranged by size:
5935 474 12
BstYI r'GATC_y
Cuts at: 1972 3564 3751 4118 4503 4520 5972 6058 6070
Size: 1592 187 367 385 17 1452 86 12
Cuts at: 6070 6416 1972
Size: 346 1977
Fragments arranged by size:
1977 1592 1452 385 367 346 187 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2208 2334 2421 2469 2526 2577 2802
Size: 552 1143 126 87 48 57 51 225
Cuts at: 2802 2940 513
Size: 138 3994
Fragments arranged by size:
3994 1143 552 225 138 126 87 57
51 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5682
Fragments arranged by size:
5682 683 56
BtsI GCAGTG_nn'
Cuts at: 3937 4731 4751 3937
Size: 794 20 5607
Fragments arranged by size:
5607 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3725 3725
Size: 6421
DraIII CAC_nnn'GTG
Cuts at: 3652 3652
Size: 6421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5428 809
Size: 507 4112 1802
Fragments arranged by size:
4112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2331 2418
Size: 54 1425 50 275 18 429 72 87
Cuts at: 2418 2424 2463 2497 2643 2937 3042 3054 3307
Size: 6 39 34 146 294 105 12 253
Cuts at: 3307 3654 3687 3868 4757 6386 8
Size: 347 33 181 889 1629 43
Fragments arranged by size:
1629 1425 889 429 347 294 275 253
181 146 105 87 72 54 50 43
39 34 33 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 2259 2643 3868 1537
Size: 275 447 384 1225 4090
Fragments arranged by size:
4090 1225 447 384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4351 1353
Size: 2998 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5008 5382 5528 387
Size: 1064 138 3419 374 146 1280
Fragments arranged by size:
3419 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2205 2205
Size: 6421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 3155 3532 4484 5081 5868 6136
Size: 393 424 1099 377 952 597 787 268
Cuts at: 6136 1239
Size: 1524
Fragments arranged by size:
1524 1099 952 787 597 424 393 377
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3341 3341
Size: 6421
EcoO109I rG'GnC_Cy
Cuts at: 2042 2122 2693 2694 2042
Size: 80 571 1 5769
Fragments arranged by size:
5769 571 80 1
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3181 3256 3181
Size: 75 6346
FspI TGC'GCA
Cuts at: 2918 2969 4927 2918
Size: 51 1958 4412
Fragments arranged by size:
4412 1958 51
HaeII r_GCGC'y
Cuts at: 1678 2445 2737 2949 2976 3283 3386 3483 3613
Size: 767 292 212 27 307 103 97 130
Cuts at: 3613 3666 5568 1678
Size: 53 1902 2531
Fragments arranged by size:
2531 1902 767 307 292 212 130 103
97 53 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3433 3465 3614 3646 5062
Size: 32 135 32 1861 32 149 32 1416
Cuts at: 5062 5094 5136 5168 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1861 1416 149 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3765 4011 669
Size: 988 281 1827 246 3079
Fragments arranged by size:
3079 1827 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KasI G'GCGC_C
Cuts at: 2945 3279 3662 2945
Size: 334 383 5704
Fragments arranged by size:
5704 383 334
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5535 5719 1305
Size: 4230 184 2007
Fragments arranged by size:
4230 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3056 10
Size: 54 1425 343 1224 3375
Fragments arranged by size:
3375 1425 1224 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2576 3062 4379 4738 4897 518
Size: 648 1161 249 486 1317 359 159 2042
Fragments arranged by size:
2042 1317 1161 648 486 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2187 2210 3133 3189 3333 3388 3532 3837
Size: 581 23 923 56 144 55 144 305
Cuts at: 3837 4514 5662 5907 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 923 677 581 305 245 144
144 56 55 23
NaeI GCC'GGC
Cuts at: 3278 3278
Size: 6421
NarI GG'CG_CC
Cuts at: 2946 3280 3663 2946
Size: 334 383 5704
Fragments arranged by size:
5704 383 334
NcoI C'CATG_G
Cuts at: 513 1065 2469 2802 513
Size: 552 1404 333 4132
Fragments arranged by size:
4132 1404 552 333
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NgoMIV G'CCGG_C
Cuts at: 3276 3276
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 2643 3868 2643
Size: 1225 5196
NspI r_CATG'y
Cuts at: 1132 1229 2968 3779 5324 1132
Size: 97 1739 811 1545 2229
Fragments arranged by size:
2229 1739 1545 811 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6421
PciI A'CATG_T
Cuts at: 5320 5320
Size: 6421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6421
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2042 2042
Size: 6421
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6421
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PspOMI G'GGCC_C
Cuts at: 2122 2693 2122
Size: 571 5850
PspXI vC'TCGA_Gb
Cuts at: 2867 2867
Size: 6421
PstI C_TGCA'G
Cuts at: 830 2388 3773 830
Size: 1558 1385 3478
Fragments arranged by size:
3478 1558 1385
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6421
PvuII CAG'CTG
Cuts at: 3133 3189 3532 3133
Size: 56 343 6022
Fragments arranged by size:
6022 343 56
SacI G_AGCT'C
Cuts at: 2207 2207
Size: 6421
SacII CC_GC'GG
Cuts at: 2211 2211
Size: 6421
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SanDI GG'GwC_CC
Cuts at: 2042 2042
Size: 6421
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SfcI C'TryA_G
Cuts at: 826 1045 2384 3694 3769 4904 5585 5776 826
Size: 219 1339 1310 75 1135 681 191 1471
Fragments arranged by size:
1471 1339 1310 1135 681 219 191 75
SfoI GGC'GCC
Cuts at: 2947 3281 3664 2947
Size: 334 383 5704
Fragments arranged by size:
5704 383 334
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 3276 1700
Size: 1576 4845
SmaI CCC'GGG
Cuts at: 1872 3748 1872
Size: 1876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 2867 3017 4525 5426 5688
Size: 197 926 666 258 150 1508 901 262
Cuts at: 5688 820
Size: 1553
Fragments arranged by size:
1553 1508 926 901 666 262 258 197
150
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 2968 3779 1132
Size: 1836 811 3774
Fragments arranged by size:
3774 1836 811
SspI AAT'ATT
Cuts at: 5 52 4345 5
Size: 47 4293 2081
Fragments arranged by size:
4293 2081 47
StuI AGG'CCT
Cuts at: 1517 2900 3677 1517
Size: 1383 777 4261
Fragments arranged by size:
4261 1383 777
StyI C'CwwG_G
Cuts at: 513 1065 2469 2802 2976 3634 3860 513
Size: 552 1404 333 174 658 226 3074
Fragments arranged by size:
3074 1404 658 552 333 226 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2645 3056 3490 3728 4448 4465 4618
Size: 1255 1065 411 434 238 720 17 153
Cuts at: 4618 4803 5248 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1065 720 445 434 411 238
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 4667 6379
Size: 274 80 33 51 1443 846 1844 1712
Cuts at: 6379 96
Size: 138
Fragments arranged by size:
1844 1712 1443 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2262 2294 2522 2554 6381 6413 2262
Size: 32 228 32 3827 32 2270
Fragments arranged by size:
3827 2270 228 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6421
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 6421
XhoI C'TCGA_G
Cuts at: 1943 2867 1943
Size: 924 5497
XmaI C'CCGG_G
Cuts at: 1870 3746 1870
Size: 1876 4545
XmnI GAAnn'nnTTC
Cuts at: 4550 4550
Size: 6421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4229 276
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PsrI
RsrII SapI SexAI SfiI SrfI SwaI