Restriction map for
pFN21AB7041
(Linear) (Six-base) MAPSORT of: KIBB7041.seq Check: 3451 from: 1 to: 1716
KIBB7041.orf
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 1672 1716
Size: 386 1286 44
Fragments arranged by size:
1286 386 44
AccI GT'mk_AC
Cuts at: 0 1298 1716
Size: 1298 418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1716
Size: 1150 566
AfeI AGC'GCT
Cuts at: 0 730 1606 1716
Size: 730 876 110
Fragments arranged by size:
876 730 110
AgeI A'CCGG_T
Cuts at: 0 578 1716
Size: 578 1138
AlwNI CAG_nnn'CTG
Cuts at: 0 560 1716
Size: 560 1156
ApaI G_GGCC'C
Cuts at: 0 121 692 1716
Size: 121 571 1024
Fragments arranged by size:
1024 571 121
AvaI C'yCGr_G
Cuts at: 0 862 1350 1432 1716
Size: 862 488 82 284
Fragments arranged by size:
862 488 284 82
AvrII C'CTAG_G
Cuts at: 0 1629 1716
Size: 1629 87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 592 625 1716
Size: 592 33 1091
Fragments arranged by size:
1091 592 33
BanI G'GyrC_C
Cuts at: 0 940 1274 1657 1716
Size: 940 334 383 59
Fragments arranged by size:
940 383 334 59
BanII G_rGCy'C
Cuts at: 0 67 121 202 692 713 1716
Size: 67 54 81 490 21 1003
Fragments arranged by size:
1003 490 81 67 54 21
BbeI G_GCGC'C
Cuts at: 0 944 1278 1661 1716
Size: 944 334 383 55
Fragments arranged by size:
944 383 334 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 759 1059 1140 1716
Size: 294 465 300 81 576
Fragments arranged by size:
576 465 300 294 81
BbvCI CC'TCA_GC
Cuts at: 0 553 1716
Size: 553 1163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1509 1543 1716
Size: 1509 34 173
Fragments arranged by size:
1509 173 34
BglI GCCn_nnn'nGGC
Cuts at: 0 649 949 1716
Size: 649 300 767
Fragments arranged by size:
767 649 300
BlpI GC'TnA_GC
Cuts at: 0 433 1716
Size: 433 1283
Bme1580I G_kGCm'C
Cuts at: 0 121 692 719 1716
Size: 121 571 27 997
Fragments arranged by size:
997 571 121 27
BmgBI CAC'GTC
Cuts at: 0 240 528 1716
Size: 240 288 1188
Fragments arranged by size:
1188 288 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1527 1716
Size: 51 1476 189
Fragments arranged by size:
1476 189 51
Bpu10I CC'TnA_GC
Cuts at: 0 19 553 1716
Size: 19 534 1163
Fragments arranged by size:
1163 534 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 997 1716
Size: 589 408 719
Fragments arranged by size:
719 589 408
BsaI GGTCTCn'nnnn_
Cuts at: 0 1506 1699 1716
Size: 1506 193 17
Fragments arranged by size:
1506 193 17
BsaAI yAC'GTr
Cuts at: 0 585 834 1716
Size: 585 249 882
Fragments arranged by size:
882 585 249
BsaHI Gr'CG_yC
Cuts at: 0 299 872 941 1145 1275 1634 1658 1716
Size: 299 573 69 204 130 359 24 58
Fragments arranged by size:
573 359 299 204 130 69 58 24
BsaWI w'CCGG_w
Cuts at: 0 194 578 1446 1508 1716
Size: 194 384 868 62 208
Fragments arranged by size:
868 384 208 194 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 1609 1639 1716
Size: 184 30 1395 30 77
Fragments arranged by size:
1395 184 77 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 762 763 1716
Size: 762 1 953
Fragments arranged by size:
953 762 1
BseYI C'CCAG_C
Cuts at: 0 612 696 1471 1497 1523 1601 1716
Size: 612 84 775 26 26 78 115
Fragments arranged by size:
775 612 115 84 78 26 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 546 1716
Size: 522 24 1170
Fragments arranged by size:
1170 522 24
BsiEI CG_ry'CG
Cuts at: 0 257 641 1451 1716
Size: 257 384 810 265
Fragments arranged by size:
810 384 265 257
BsiHKAI G_wGCw'C
Cuts at: 0 202 784 1076 1716
Size: 202 582 292 640
Fragments arranged by size:
640 582 292 202
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1249 1716
Size: 1249 467
Bsp1286I G_dGCh'C
Cuts at: 0 67 121 202 692 713 719 784 1076
Size: 67 54 81 490 21 6 65 292
Cuts at: 1076 1716
Size: 640
Fragments arranged by size:
640 490 292 81 67 65 54 21
6
BspEI T'CCGG_A
Cuts at: 0 1508 1716
Size: 1508 208
BspHI T'CATG_A
Cuts at: 0 620 1716
Size: 620 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 325 350 386 434 800 1672 1716
Size: 325 25 36 48 366 872 44
Fragments arranged by size:
872 366 325 48 44 36 25
BsrBI CCG'CTC
Cuts at: 0 1107 1197 1716
Size: 1107 90 519
Fragments arranged by size:
1107 519 90
BsrFI r'CCGG_y
Cuts at: 0 578 1271 1716
Size: 578 693 445
Fragments arranged by size:
693 578 445
BssHII G'CGCG_C
Cuts at: 0 278 841 1230 1530 1574 1581 1716
Size: 278 563 389 300 44 7 135
Fragments arranged by size:
563 389 300 278 135 44 7
BssSI C'ACGA_G
Cuts at: 0 1642 1716
Size: 1642 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 960 1716
Size: 960 756
BstEII G'GTnAC_C
Cuts at: 0 337 1716
Size: 337 1379
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 1047 1059 1716
Size: 573 474 12 657
Fragments arranged by size:
657 573 474 12
BstYI r'GATC_y
Cuts at: 0 1559 1716
Size: 1559 157
BtgI C'CryG_G
Cuts at: 0 203 329 416 464 521 572 797 935
Size: 203 126 87 48 57 51 225 138
Cuts at: 935 1716
Size: 781
Fragments arranged by size:
781 225 203 138 126 87 57 51
48
DraIII CAC_nnn'GTG
Cuts at: 0 1647 1716
Size: 1647 69
EaeI y'GGCC_r
Cuts at: 0 254 326 413 419 458 492 638 932
Size: 254 72 87 6 39 34 146 294
Cuts at: 932 1037 1049 1302 1649 1682 1716
Size: 105 12 253 347 33 34
Fragments arranged by size:
347 294 254 253 146 105 87 72
39 34 34 33 12 6
EagI C'GGCC_G
Cuts at: 0 254 638 1716
Size: 254 384 1078
Fragments arranged by size:
1078 384 254
EcoICRI GAG'CTC
Cuts at: 0 200 1716
Size: 200 1516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1150 1527 1716
Size: 51 1099 377 189
Fragments arranged by size:
1099 377 189 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1336 1716
Size: 1336 380
EcoO109I rG'GnC_Cy
Cuts at: 0 37 117 688 689 1716
Size: 37 80 571 1 1027
Fragments arranged by size:
1027 571 80 37 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1176 1251 1716
Size: 1176 75 465
Fragments arranged by size:
1176 465 75
FspI TGC'GCA
Cuts at: 0 913 964 1716
Size: 913 51 752
Fragments arranged by size:
913 752 51
HaeII r_GCGC'y
Cuts at: 0 440 732 944 971 1278 1381 1478 1608
Size: 440 292 212 27 307 103 97 130
Cuts at: 1608 1661 1716
Size: 53 55
Fragments arranged by size:
440 307 292 212 130 103 97 55
53 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1428 1460 1609 1641 1716
Size: 1428 32 149 32 75
Fragments arranged by size:
1428 149 75 32 32
KasI G'GCGC_C
Cuts at: 0 940 1274 1657 1716
Size: 940 334 383 59
Fragments arranged by size:
940 383 334 59
MscI TGG'CCA
Cuts at: 0 1051 1716
Size: 1051 665
MslI CAynn'nnrTG
Cuts at: 0 322 571 1057 1716
Size: 322 249 486 659
Fragments arranged by size:
659 486 322 249
MspA1I CmG'CkG
Cuts at: 0 182 205 1128 1184 1328 1383 1527 1716
Size: 182 23 923 56 144 55 144 189
Fragments arranged by size:
923 189 182 144 144 56 55 23
NaeI GCC'GGC
Cuts at: 0 1273 1716
Size: 1273 443
NarI GG'CG_CC
Cuts at: 0 941 1275 1658 1716
Size: 941 334 383 58
Fragments arranged by size:
941 383 334 58
NcoI C'CATG_G
Cuts at: 0 464 797 1716
Size: 464 333 919
Fragments arranged by size:
919 464 333
NgoMIV G'CCGG_C
Cuts at: 0 1271 1716
Size: 1271 445
NotI GC'GGCC_GC
Cuts at: 0 638 1716
Size: 638 1078
NspI r_CATG'y
Cuts at: 0 963 1716
Size: 963 753
PpuMI rG'GwC_Cy
Cuts at: 0 37 1716
Size: 37 1679
PspOMI G'GGCC_C
Cuts at: 0 117 688 1716
Size: 117 571 1028
Fragments arranged by size:
1028 571 117
PspXI vC'TCGA_Gb
Cuts at: 0 862 1716
Size: 862 854
PstI C_TGCA'G
Cuts at: 0 383 1716
Size: 383 1333
PvuII CAG'CTG
Cuts at: 0 1128 1184 1527 1716
Size: 1128 56 343 189
Fragments arranged by size:
1128 343 189 56
SacI G_AGCT'C
Cuts at: 0 202 1716
Size: 202 1514
SacII CC_GC'GG
Cuts at: 0 206 1716
Size: 206 1510
SanDI GG'GwC_CC
Cuts at: 0 37 1716
Size: 37 1679
SfcI C'TryA_G
Cuts at: 0 379 1689 1716
Size: 379 1310 27
Fragments arranged by size:
1310 379 27
SfoI GGC'GCC
Cuts at: 0 942 1276 1659 1716
Size: 942 334 383 57
Fragments arranged by size:
942 383 334 57
SgrAI Cr'CCGG_yG
Cuts at: 0 1271 1716
Size: 1271 445
SmlI C'TyrA_G
Cuts at: 0 604 862 1012 1716
Size: 604 258 150 704
Fragments arranged by size:
704 604 258 150
SphI G_CATG'C
Cuts at: 0 963 1716
Size: 963 753
StuI AGG'CCT
Cuts at: 0 895 1672 1716
Size: 895 777 44
Fragments arranged by size:
895 777 44
StyI C'CwwG_G
Cuts at: 0 464 797 971 1629 1716
Size: 464 333 174 658 87
Fragments arranged by size:
658 464 333 174 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 640 1051 1485 1716
Size: 640 411 434 231
Fragments arranged by size:
640 434 411 231
TatI w'GTAC_w
Cuts at: 0 818 1716
Size: 818 898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 257 289 517 549 1716
Size: 257 32 228 32 1167
Fragments arranged by size:
1167 257 228 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 1716
Size: 471 1245
XhoI C'TCGA_G
Cuts at: 0 862 1716
Size: 862 854
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AlwNI ApaI AvaI
AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI DraIII
EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SanDI SfcI SfoI SgrAI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI BamHI BciVI
BclI BfrBI BglII BmrI BmtI BplI BsaBI BsiWI
BsmI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EarI EciI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI
SgfI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XmaI XmnI ZraI