(Circular) (Six-base) MAPSORT of: pFN21AE3510.seq Check: 200 from: 1 to: 5914 pFN21AE3510 5914 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2313 3724 278 Size: 53 83 186 936 777 1411 2468 Fragments arranged by size: 2468 1411 936 777 186 83 53 AccI GT'mk_AC Cuts at: 1937 3257 1937 Size: 1320 4594 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2683 3078 3977 5361 5629 1632 Size: 1051 395 899 1384 268 1917 Fragments arranged by size: 1917 1384 1051 899 395 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 4643 4643 Size: 5914 AleI CACnn'nnGTG Cuts at: 1166 2141 1166 Size: 975 4939 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5914 AlwNI CAG_nnn'CTG Cuts at: 2122 3657 5229 2122 Size: 1535 1572 2807 Fragments arranged by size: 2807 1572 1535 ApaI G_GGCC'C Cuts at: 3187 3187 Size: 5914 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 3223 3449 3601 5710 1493 Size: 1730 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 2928 3239 1870 Size: 73 985 311 4545 Fragments arranged by size: 4545 985 311 73 BamHI G'GATC_C Cuts at: 2539 3244 3611 2539 Size: 705 367 4842 Fragments arranged by size: 4842 705 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3235 4690 618 Size: 325 217 627 1448 1455 1842 Fragments arranged by size: 1842 1455 1448 627 325 217 BanII G_rGCy'C Cuts at: 1391 2184 2370 2613 3187 3233 1391 Size: 793 186 243 574 46 4072 Fragments arranged by size: 4072 793 574 243 186 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4943 BbvCI CC'TCA_GC Cuts at: 2418 2634 2657 2418 Size: 216 23 5675 Fragments arranged by size: 5675 216 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3020 3054 4105 4139 1903 Size: 34 1083 34 1051 34 3678 Fragments arranged by size: 3678 1083 1051 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 2877 1548 Size: 1329 4585 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4525 136 Size: 107 122 71 631 605 2853 1525 Fragments arranged by size: 2853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5909 5909 Size: 5914 BlpI GC'TnA_GC Cuts at: 3331 3331 Size: 5914 Bme1580I G_kGCm'C Cuts at: 1790 3175 3187 3975 5131 1790 Size: 1385 12 788 1156 2573 Fragments arranged by size: 2573 1385 1156 788 12 BmgBI CAC'GTC Cuts at: 1318 2707 1318 Size: 1389 4525 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2059 3645 4603 Size: 416 53 83 399 24 642 1586 958 Cuts at: 4603 442 Size: 1753 Fragments arranged by size: 1753 1586 958 642 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2384 2384 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 4574 1239 Size: 1115 2220 2579 Fragments arranged by size: 2579 2220 1115 Bpu10I CC'TnA_GC Cuts at: 2418 2634 2657 2418 Size: 216 23 5675 Fragments arranged by size: 5675 216 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4039 4904 5202 4039 Size: 865 298 4751 Fragments arranged by size: 4751 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2886 4577 882 Size: 617 1387 1691 2219 Fragments arranged by size: 2219 1691 1387 617 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 2387 2585 5908 2387 Size: 198 3323 2393 Fragments arranged by size: 3323 2393 198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2310 3721 4103 4738 Size: 53 83 186 936 777 1411 382 635 Cuts at: 4738 275 Size: 1451 Fragments arranged by size: 1451 1411 936 777 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2013 4347 4745 5019 5166 5577 2013 Size: 2334 398 274 147 411 2350 Fragments arranged by size: 2350 2334 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5689 Fragments arranged by size: 5689 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2387 1186 Size: 1027 174 4713 Fragments arranged by size: 4713 1027 174 BseYI C'CCAG_C Cuts at: 3003 5117 5613 3003 Size: 2114 496 3304 Fragments arranged by size: 3304 2114 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 3135 1163 Size: 573 672 727 3942 Fragments arranged by size: 3942 727 672 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2613 3975 4060 5131 2613 Size: 1362 85 1071 3396 Fragments arranged by size: 3396 1362 1071 85 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2370 2613 3175 3187 3233 3975 Size: 399 394 186 243 562 12 46 742 Cuts at: 3975 4060 5131 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 399 394 243 186 85 46 12 BspEI T'CCGG_A Cuts at: 2013 5577 2013 Size: 3564 2350 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3269 844 Size: 2425 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3146 3801 1394 Size: 429 1323 655 3507 Fragments arranged by size: 3507 1323 655 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2321 4409 4583 65 Size: 1183 1073 2088 174 1396 Fragments arranged by size: 2088 1396 1183 1073 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4558 4745 1700 Size: 110 2748 187 2869 Fragments arranged by size: 2869 2748 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 2710 794 Size: 1916 3998 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2143 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2539 2847 3244 3611 3996 4013 5465 5551 Size: 567 308 397 367 385 17 1452 86 Cuts at: 5551 5563 5909 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 567 397 385 367 346 308 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2525 2853 2525 Size: 328 5586 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5175 Fragments arranged by size: 5175 683 56 BtsI GCAGTG_nn' Cuts at: 3123 3430 4224 4244 3123 Size: 307 794 20 4793 Fragments arranged by size: 4793 794 307 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DraIII CAC_nnn'GTG Cuts at: 2710 2710 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2453 4921 809 Size: 507 1137 2468 1802 Fragments arranged by size: 2468 1802 1137 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 3361 4250 Size: 54 1425 50 275 18 214 1317 889 Cuts at: 4250 5879 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1317 889 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3844 1353 Size: 2491 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2611 2611 Size: 5914 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2683 3078 3977 4574 5361 5629 Size: 393 722 329 395 899 597 787 268 Cuts at: 5629 1239 Size: 1524 Fragments arranged by size: 1524 899 787 722 597 395 393 329 268 EcoNI CCTnn'n_nnAGG Cuts at: 2256 2256 Size: 5914 EcoO109I rG'GnC_Cy Cuts at: 3184 3184 Size: 5914 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2092 2092 Size: 5914 FspI TGC'GCA Cuts at: 2022 4420 2022 Size: 2398 3516 HaeII r_GCGC'y Cuts at: 1678 5061 1678 Size: 3383 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2807 2839 2981 3013 4555 Size: 32 135 32 1235 32 142 32 1542 Cuts at: 4555 4587 4629 4661 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1542 1235 142 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2567 5028 5212 1305 Size: 1262 2461 184 2007 Fragments arranged by size: 2461 2007 1262 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4092 Fragments arranged by size: 4092 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 3872 4231 4390 518 Size: 648 975 1731 359 159 2042 Fragments arranged by size: 2042 1731 975 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2422 2661 2939 3034 3199 3205 3330 4007 Size: 816 239 278 95 165 6 125 677 Cuts at: 4007 5155 5400 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 278 245 239 165 125 95 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NruI TCG'CGA Cuts at: 2787 2787 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2347 2687 3272 4817 1132 Size: 97 1118 340 585 1545 2229 Fragments arranged by size: 2229 1545 1118 585 340 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PfoI T'CCnGG_A Cuts at: 2370 2799 2370 Size: 429 5485 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PspOMI G'GGCC_C Cuts at: 3183 3183 Size: 5914 PstI C_TGCA'G Cuts at: 830 3204 3266 830 Size: 2374 62 3478 Fragments arranged by size: 3478 2374 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2422 2661 3199 3205 2422 Size: 239 538 6 5131 Fragments arranged by size: 5131 538 239 6 SacI G_AGCT'C Cuts at: 2613 2613 Size: 5914 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SexAI A'CCwGG_T Cuts at: 2595 2672 2595 Size: 77 5837 SfcI C'TryA_G Cuts at: 826 1045 3200 3262 4397 5078 5269 826 Size: 219 2155 62 1135 681 191 1471 Fragments arranged by size: 2155 1471 1135 681 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4018 4919 5181 820 Size: 197 926 2075 901 262 1553 Fragments arranged by size: 2075 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 2347 3272 1132 Size: 1215 925 3774 Fragments arranged by size: 3774 1215 925 SspI AAT'ATT Cuts at: 5 52 3838 5 Size: 47 3786 2081 Fragments arranged by size: 3786 2081 47 StuI AGG'CCT Cuts at: 1517 3013 1517 Size: 1496 4418 StyI C'CwwG_G Cuts at: 513 1065 2004 3089 3353 513 Size: 552 939 1085 264 3074 Fragments arranged by size: 3074 1085 939 552 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2757 2815 3941 3958 4111 4296 4741 Size: 1255 1177 58 1126 17 153 185 445 Cuts at: 4741 325 Size: 1498 Fragments arranged by size: 1498 1255 1177 1126 445 185 153 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4160 5872 96 Size: 274 80 33 51 1443 2183 1712 138 Fragments arranged by size: 2183 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2825 2857 5874 5906 2825 Size: 32 3017 32 2833 Fragments arranged by size: 3017 2833 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5914 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 4043 4043 Size: 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2311 3722 276 Size: 53 83 186 936 777 1411 2468 Fragments arranged by size: 2468 1411 936 777 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI