################################## # NO CUT ENZYMES ( 215 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MamI MboI MflI MluI MluNI MnlI MroI MscI MseI MslI MspI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 155 GCGG CCGC(-3/-1) AluI 183 AGCT AG^CT BbsI 297 GTCTTC GAAGAC(2/6) BpuAI 297 GTCTTC GAAGAC(2/6) BsaI 51 GGTCTC GGTCTC(1/5) BsmAI 52 GTCTC GTCTC(1/5) BsrDI 148 GCAATG GCAATG(2/0) BsrI 328 ACTGG ACTGG(1/-1) BssKI 39 CCTGG ^CC[ACGT]GG BstNI 39 CCTGG CC^[AT]GG Cfr13I 42 GGCCC G^G[ACGT]CC DdeI 54 CTCAG C^T[ACGT]AG EarI 121 GAAGAG CTCTTC(1/4) EcoRII 39 CCTGG ^CC[AT]GG EcoT22I 127 ATGCAT ATGCA^T Fnu4HI 155 GCGGC GC^[ACGT]GC HaeIII 42 GGCC GG^CC HphI 17 TCACC GGTGA(8/7) ItaI 155 GCGGC GC^[ACGT]GC Ksp632I 121 GAAGAG CTCTTC(1/4) MaeIII 247 GTAAC ^GT[ACGT]AC MfeI 46 CAATTG C^AATTG MspA1I 182 CAGCTG C[AC]G^C[GT]G MunI 46 CAATTG C^AATTG MvaI 39 CCTGG CC^[AT]GG MwoI 253 GCAACAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 130 CATG CATG^ NlaIV 306 GGCTCC GG[ACGT]^[ACGT]CC NsiI 127 ATGCAT ATGCA^T NspI 129 GCATGT [GA]CATG^[CT] PvuII 182 CAGCTG CAG^CTG Sau96I 42 GGCCC G^G[ACGT]CC ScrFI 39 CCTGG CC^[ACGT]GG SfaNI 126 GATGC GCATC(5/9) TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### AcsI 95 GAATTT [GA]^AATT[CT] 236 GAATTT ApoI 95 GAATTT [GA]^AATT[CT] 236 GAATTT BsmFI 167 GGGAC GGGAC(10/14) 6 GTCCC MaeII 89 ACGT A^CGT 192 ACGT TaqI 292 TCGA T^CGA 301 TCGA TthHB8I 292 TCGA T^CGA 301 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MboII Tsp509I