Restriction map for
pFN21AE3962
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BbeI BbrPI BcgI BclI
BfaI BfrI BglI BglII BlnI
BsaBI BsaHI BseAI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
Hin1I HincII HindII HinfI HpaI
KasI KpnI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SexAI SfiI SfoI
SfuI SpeI SphI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 1262 ACATGT A^C[GA][CT]GT
Alw44I 1426 GTGCAC G^TGCAC
AocI 1163 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1397 AGCGCT AGC^GCT
ApaLI 1426 GTGCAC G^TGCAC
AvaII 1128 GGTCC G^G[AT]CC
BamHI 946 GGATCC G^GATCC
BciVI 862 GTATCC GTATCC(6/5)
BlpI 1192 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1192 GCTGAGC GC^T[ACGT]AGC
BsaAI 1203 TACGTA [CT]AC^GT[GA]
BsaWI 216 TCCGGT [AT]^CCGG[AT]
BseRI 760 GAGGAG GAGGAG(10/8)
Bsp1407I 260 TGTACA T^GTACA
BspLU11I 1262 ACATGT A^CATGT
BsrGI 260 TGTACA T^GTACA
BstAPI 231 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1163 CCTCAGG CC^T[ACGT]AGG
CelII 1192 GCTGAGC GC^T[ACGT]AGC
Eco47III 1397 AGCGCT AGC^GCT
Eco81I 1163 CCTCAGG CC^T[ACGT]AGG
EcoT14I 563 CCAAGG C^C[AT][AT]GG
HindIII 122 AAGCTT A^AGCTT
KspI 599 CCGCGG CCGC^GG
NaeI 157 GCCGGC GCC^GGC
NgoMIV 157 GCCGGC G^CCGGC
PaeR7I 374 CTCGAG C^TCGAG
PflMI 462 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1127 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 915 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1413 GAGCTC GAGCT^C
SacII 599 CCGCGG CCGC^GG
SapI 1410 GAAGAGC GCTCTTC(1/4)
SbfI 297 CCTGCAGG CCTGCA^GG
ScaI 1111 AGTACT AGT^ACT
SgrAI 1359 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 1203 TACGTA TAC^GTA
Sse8387I 297 CCTGCAGG CCTGCA^GG
SspBI 260 TGTACA T^GTACA
StyI 563 CCAAGG C^C[AT][AT]GG
Van91I 462 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1109 CCAGTACTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 374 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 261 GTAC GT^AC
1112 GTAC
BbsI 966 GAAGAC GAAGAC(2/6)
1480 GTCTTC
BbvCI 568 GCTGAGG CCTCAGC(-5/-2)
835 GCTGAGG
BpmI 1090 CTCCAG CTGGAG(16/14)
1501 CTGGAG
BpuAI 966 GAAGAC GAAGAC(2/6)
1480 GTCTTC
BsiEI 155 CGGCCG CG[GA][CT]^CG
311 CGGCCG
BsmI 349 GAATGC GAATGC(1/-1)
113 GCATTC
BsrFI 157 GCCGGC [GA]^CCGG[CT]
1360 ACCGGC
BstYI 203 AGATCC [GA]^GATC[CT]
946 GGATCC
Cfr10I 157 GCCGGC [GA]^CCGG[CT]
1360 ACCGGC
DraII 573 GGGGCCT [GA]G^G[ACGT]CC[CT]
1127 AGGTCCC
DsaI 599 CCGCGG C^C[GA][CT]GG
1154 CCACGG
EagI 155 CGGCCG C^GGCCG
311 CGGCCG
EarI 991 CTCTTC CTCTTC(1/4)
1410 GAAGAG
EclXI 155 CGGCCG C^GGCCG
311 CGGCCG
Eco52I 155 CGGCCG C^GGCCG
311 CGGCCG
EcoO109I 573 GGGGCCT [GA]G^G[ACGT]CC[CT]
1127 AGGTCCC
HgaI 316 GCGTC GACGC(5/10)
447 GCGTC
Ksp632I 991 CTCTTC CTCTTC(1/4)
1410 GAAGAG
MflI 203 AGATCC [GA]^GATC[CT]
946 GGATCC
NspI 1262 ACATGT [GA]CATG^[CT]
1430 ACATGC
RsaI 261 GTAC GT^AC
1112 GTAC
SfaNI 580 GATGC GCATC(5/9)
21 GCATC
SmaI 381 CCCGGG CCC^GGG
483 CCCGGG
SmlI 58 CTTGAG C^T[CT][GA]AG
374 CTCGAG
TaqI 344 TCGA T^CGA
375 TCGA
TthHB8I 344 TCGA T^CGA
375 TCGA
XhoII 203 AGATCC [GA]^GATC[CT]
946 GGATCC
XmaCI 381 CCCGGG C^CCGGG
483 CCCGGG
XmaI 381 CCCGGG C^CCGGG
483 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaI AviII BanI BanII
BbvI BcnI BmyI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII Sau3AI Sau96I ScrFI SfcI
TseI Tsp45I TspRI