(Circular) (Six-base) MAPSORT of: pFN21AE3250.seq Check: 2882 from: 1 to: 6436 pFN21AE3250 6436 bp With 182 enzymes: SgfI * March 28, 2012 14:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3779 1937 Size: 1842 4594 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3346 4499 5883 6151 1632 Size: 1714 1153 1384 268 1917 Fragments arranged by size: 1917 1714 1384 1153 268 AflII C'TTAA_G Cuts at: 820 1017 3446 820 Size: 197 2429 3810 Fragments arranged by size: 3810 2429 197 AflIII A'CryG_T Cuts at: 1203 2459 5335 6312 1203 Size: 1256 2876 977 1327 Fragments arranged by size: 2876 1327 1256 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 2460 1166 Size: 1294 5142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AlwNI CAG_nnn'CTG Cuts at: 4179 5751 4179 Size: 1572 4864 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 2307 2918 3745 3971 4123 6232 1493 Size: 814 611 827 226 152 2109 1697 Fragments arranged by size: 2109 1697 827 814 611 226 152 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2009 2020 3761 1870 Size: 73 66 11 1741 4545 Fragments arranged by size: 4545 1741 73 66 11 BamHI G'GATC_C Cuts at: 3766 4133 3766 Size: 367 6069 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2524 3757 5212 618 Size: 325 217 627 737 1233 1455 1842 Fragments arranged by size: 1842 1455 1233 737 627 325 217 BanII G_rGCy'C Cuts at: 1391 3755 1391 Size: 2364 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4627 4661 1903 Size: 34 50 34 2606 34 3678 Fragments arranged by size: 3678 2606 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4328 5538 4328 Size: 1210 5226 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5047 136 Size: 107 122 71 631 605 3375 1525 Fragments arranged by size: 3375 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6431 6431 Size: 6436 BlpI GC'TnA_GC Cuts at: 3853 3853 Size: 6436 Bme1580I G_kGCm'C Cuts at: 1790 2990 4497 5653 1790 Size: 1200 1507 1156 2573 Fragments arranged by size: 2573 1507 1200 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2743 4167 5125 Size: 416 53 83 399 24 1326 1424 958 Cuts at: 5125 442 Size: 1753 Fragments arranged by size: 1753 1424 1326 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2334 2920 2988 3072 3156 3240 3256 Size: 1013 82 586 68 84 84 84 16 Cuts at: 3256 3324 3340 3492 3576 5096 1239 Size: 68 16 152 84 1520 2579 Fragments arranged by size: 2579 1520 1013 586 152 84 84 84 84 82 68 68 16 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2419 4561 5426 5724 2419 Size: 2142 865 298 3131 Fragments arranged by size: 3131 2142 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5099 882 Size: 617 3600 2219 Fragments arranged by size: 3600 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4227 493 Size: 711 3023 2702 Fragments arranged by size: 3023 2702 711 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2655 4243 4625 5260 Size: 53 83 186 936 1122 1588 382 635 Cuts at: 5260 275 Size: 1451 Fragments arranged by size: 1588 1451 1122 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3288 4869 5267 5541 5688 6099 3288 Size: 1581 398 274 147 411 3625 Fragments arranged by size: 3625 1581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2307 2337 1178 Size: 30 165 30 904 30 5277 Fragments arranged by size: 5277 904 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6436 BseYI C'CCAG_C Cuts at: 5639 6135 5639 Size: 496 5940 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2839 3343 3511 3595 3679 1163 Size: 573 1103 504 168 84 84 3920 Fragments arranged by size: 3920 1103 573 504 168 84 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 3886 4647 5675 1540 Size: 275 186 72 1813 761 1028 2301 Fragments arranged by size: 2301 1813 1028 761 275 186 72 BsiHKAI G_wGCw'C Cuts at: 4497 4582 5653 4497 Size: 85 1071 5280 Fragments arranged by size: 5280 1071 85 BsmI GAATG_Cn' Cuts at: 2371 2906 3158 3947 4040 2371 Size: 535 252 789 93 4767 Fragments arranged by size: 4767 789 535 252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2990 3755 4497 4582 5653 1391 Size: 399 1200 765 742 85 1071 2174 Fragments arranged by size: 2174 1200 1071 765 742 399 85 BspEI T'CCGG_A Cuts at: 6099 6099 Size: 6436 BspHI T'CATG_A Cuts at: 3232 4325 6055 3232 Size: 1093 1730 3613 Fragments arranged by size: 3613 1730 1093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2609 3791 844 Size: 1765 1182 3489 Fragments arranged by size: 3489 1765 1182 BsrBI CCG'CTC Cuts at: 1394 1823 4323 1394 Size: 429 2500 3507 Fragments arranged by size: 3507 2500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2911 3247 4931 5105 65 Size: 1183 1663 336 1684 174 1396 Fragments arranged by size: 1684 1663 1396 1183 336 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5080 5267 1700 Size: 110 3270 187 2869 Fragments arranged by size: 3270 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 4496 5508 4496 Size: 1012 5424 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6436 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstYI r'GATC_y Cuts at: 1972 2185 3766 4133 4518 4535 5987 6073 6085 Size: 213 1581 367 385 17 1452 86 12 Cuts at: 6085 6431 1972 Size: 346 1977 Fragments arranged by size: 1977 1581 1452 385 367 346 213 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5884 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3104 487 Size: 683 56 1878 3819 Fragments arranged by size: 3819 1878 683 56 BtsI GCAGTG_nn' Cuts at: 2547 3499 3583 3952 4746 4766 2547 Size: 952 84 369 794 20 4217 Fragments arranged by size: 4217 952 794 369 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2893 2928 3229 3264 529 Size: 35 2329 35 301 35 3701 Fragments arranged by size: 3701 2329 301 35 35 35 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5443 809 Size: 507 4127 1802 Fragments arranged by size: 4127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2628 3883 Size: 54 1425 50 275 18 327 471 1255 Cuts at: 3883 4772 6401 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1255 889 471 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4366 1353 Size: 3013 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 5023 5397 5543 387 Size: 1064 138 450 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 450 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2334 2920 2988 3072 3156 3240 Size: 393 620 82 586 68 84 84 84 Cuts at: 3240 3256 3324 3340 3346 3492 3576 4499 5096 Size: 16 68 16 6 146 84 923 597 Cuts at: 5096 5883 6151 1239 Size: 787 268 1524 Fragments arranged by size: 1524 923 787 620 597 586 393 268 146 84 84 84 84 82 68 68 16 16 6 EcoNI CCTnn'n_nnAGG Cuts at: 2195 2195 Size: 6436 EcoO109I rG'GnC_Cy Cuts at: 2083 2083 Size: 6436 EcoRI G'AATT_C Cuts at: 3745 4123 3745 Size: 378 6058 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 5583 1678 Size: 3905 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 2841 2873 5077 Size: 32 135 32 965 32 272 32 2204 Cuts at: 5077 5109 5151 5183 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2204 965 272 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3780 4026 669 Size: 988 281 1842 246 3079 Fragments arranged by size: 3079 1842 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6436 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 3129 3465 5550 5734 1305 Size: 1260 564 336 2085 184 2007 Fragments arranged by size: 2085 2007 1260 564 336 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 10 Size: 54 1425 343 798 3816 Fragments arranged by size: 3816 1425 798 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 4394 4753 4912 518 Size: 648 1294 1934 359 159 2042 Fragments arranged by size: 2042 1934 1294 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3852 4529 5677 5922 1606 Size: 2246 677 1148 245 2120 Fragments arranged by size: 2246 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5884 NdeI CA'TA_TG Cuts at: 387 387 Size: 6436 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 2463 2841 3794 5339 1132 Size: 97 1234 378 953 1545 2229 Fragments arranged by size: 2229 1545 1234 953 378 97 PasI CC'CwG_GG Cuts at: 1348 2257 1348 Size: 909 5527 PciI A'CATG_T Cuts at: 2459 5335 2459 Size: 2876 3560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6436 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2083 Size: 6436 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PstI C_TGCA'G Cuts at: 830 2606 3788 830 Size: 1776 1182 3478 Fragments arranged by size: 3478 1776 1182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SanDI GG'GwC_CC Cuts at: 2083 2083 Size: 6436 SbfI CC_TGCA'GG Cuts at: 2606 3788 2606 Size: 1182 5254 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SfcI C'TryA_G Cuts at: 826 1045 2106 2602 3784 4919 5600 5791 826 Size: 219 1061 496 1182 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 1061 681 496 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 2022 3763 1872 Size: 150 1741 4545 Fragments arranged by size: 4545 1741 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 2398 3446 4540 5441 5703 820 Size: 197 926 455 1048 1094 901 262 1553 Fragments arranged by size: 1553 1094 1048 926 901 455 262 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 4360 5 Size: 47 4308 2081 Fragments arranged by size: 4308 2081 47 StuI AGG'CCT Cuts at: 1517 2893 2977 3061 3145 3397 3565 3649 1517 Size: 1376 84 84 84 252 168 84 4304 Fragments arranged by size: 4304 1376 252 168 84 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2428 3875 513 Size: 552 1363 1447 3074 Fragments arranged by size: 3074 1447 1363 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4463 4480 4633 4818 5263 325 Size: 1255 2883 17 153 185 445 1498 Fragments arranged by size: 2883 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 2214 4682 Size: 274 80 33 51 1443 186 51 2468 Cuts at: 4682 6394 96 Size: 1712 138 Fragments arranged by size: 2468 1712 1443 274 186 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2307 2339 6396 6428 2307 Size: 32 4057 32 2315 Fragments arranged by size: 4057 2315 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 2020 3761 1870 Size: 150 1741 4545 Fragments arranged by size: 4545 1741 150 XmnI GAAnn'nnTTC Cuts at: 2621 4565 2621 Size: 1944 4492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI