################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HgaI HindIII HpaI HphI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 791 TCCGGA T^CCGGA AcyI 314 GGCGCC G[GA]^CG[CT]C AlwNI 670 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 341 AGCGCT AGC^GCT AviII 548 TGCGCA TGC^GCA BamHI 686 GGATCC G^GATCC BbeI 314 GGCGCC GGCGC^C BlpI 376 GCTAAGC GC^T[ACGT]AGC Bpu1102I 376 GCTAAGC GC^T[ACGT]AGC BsaHI 314 GGCGCC G[GA]^CG[CT]C BsaWI 791 TCCGGA [AT]^CCGG[AT] BseAI 791 TCCGGA T^CCGGA BspDI 244 ATCGAT AT^CGAT BspEI 791 TCCGGA T^CCGGA BspMI 221 ACCTGC ACCTGC(4/8) BsrBI 310 GAGCGG CCGCTC(-3/-3) BsrI 683 ACTGG ACTGG(1/-1) CelII 376 GCTAAGC GC^T[ACGT]AGC ClaI 244 ATCGAT AT^CGAT DraII 472 GGGGCCC [GA]G^G[ACGT]CC[CT] DsaI 134 CCGTGG C^C[GA][CT]GG Eco47III 341 AGCGCT AGC^GCT EcoNI 492 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 472 GGGGCCC [GA]G^G[ACGT]CC[CT] FspI 548 TGCGCA TGC^GCA Hin1I 314 GGCGCC G[GA]^CG[CT]C HincII 181 GTTGAC GT[CT]^[GA]AC HindII 181 GTTGAC GT[CT]^[GA]AC HinfI 700 GAGTC G^A[ACGT]TC KasI 314 GGCGCC G^GCGCC MroI 791 TCCGGA T^CCGGA NarI 314 GGCGCC GG^CGCC PflMI 690 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 700 GAGTC GAGTC(4/5) PstI 232 CTGCAG CTGCA^G PvuII 694 CAGCTG CAG^CTG SapI 79 GAAGAGC GCTCTTC(1/4) SexAI 491 ACCTGGT A^CC[AT]GGT SfcI 232 CTGCAG C^T[GA][CT]AG SfoI 314 GGCGCC GGC^GCC Van91I 690 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 250 GTAC GT^AC 762 GTAC ApaI 473 GGGCCC GGGCC^C 611 GGGCCC BanI 138 GGTGCC G^G[CT][GA]CC 314 GGCGCC BanII 473 GGGCCC G[GA]GC[CT]^C 611 GGGCCC BcnI 609 CCGGG CC^[GC]GG 614 CCCGG BmyI 473 GGGCCC G[AGT]GC[ACT]^C 611 GGGCCC BpmI 226 CTGGAG CTGGAG(16/14) 697 CTGGAG Bsp1286I 473 GGGCCC G[AGT]GC[ACT]^C 611 GGGCCC Bsp1407I 249 TGTACA T^GTACA 761 TGTACA BsrFI 90 GCCGGT [GA]^CCGG[CT] 317 GCCGGT BsrGI 249 TGTACA T^GTACA 761 TGTACA BstYI 161 AGATCC [GA]^GATC[CT] 686 GGATCC Cfr10I 90 GCCGGT [GA]^CCGG[CT] 317 GCCGGT HaeII 314 GGCGCC [GA]GCGC^[CT] 341 AGCGCT MaeII 369 ACGT A^CGT 755 ACGT MflI 161 AGATCC [GA]^GATC[CT] 686 GGATCC NciI 609 CCGGG CC^[GC]GG 614 CCCGG RsaI 250 GTAC GT^AC 762 GTAC SspBI 249 TGTACA T^GTACA 761 TGTACA XhoII 161 AGATCC [GA]^GATC[CT] 686 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AccII AciI AluI AlwI BbvI BsaJI BseRI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I