(Circular) (Six-base) MAPSORT of: pFN21AE2511.seq Check: 9573 from: 1 to: 5539 pFN21AE2511 5539 bp With 182 enzymes: SgfI * February 25, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3349 278 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2882 1937 Size: 945 4594 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 3666 4039 1563 Size: 2103 373 3063 Fragments arranged by size: 3063 2103 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2335 3602 4986 5254 1632 Size: 703 1267 1384 268 1917 Fragments arranged by size: 1917 1384 1267 703 268 AfeI AGC'GCT Cuts at: 2348 2348 Size: 5539 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5342 AflIII A'CryG_T Cuts at: 1203 4438 5415 1203 Size: 3235 977 1327 Fragments arranged by size: 3235 1327 977 AgeI A'CCGG_T Cuts at: 4370 4370 Size: 5539 AhdI GACnn_n'nnGTC Cuts at: 4268 4268 Size: 5539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5539 AlwNI CAG_nnn'CTG Cuts at: 2680 3282 4854 2680 Size: 602 1572 3365 Fragments arranged by size: 3365 1572 602 ApaI G_GGCC'C Cuts at: 2482 2620 2482 Size: 138 5401 ApaLI G'TGCA_C Cuts at: 3596 4752 3596 Size: 1156 4383 ApoI r'AATT_y Cuts at: 1493 2848 3074 3226 5335 1493 Size: 1355 226 152 2109 1697 Fragments arranged by size: 2109 1697 1355 226 152 AseI AT'TA_AT Cuts at: 160 1032 4093 160 Size: 872 3061 1606 Fragments arranged by size: 3061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2864 1870 Size: 73 921 4545 Fragments arranged by size: 4545 921 73 BamHI G'GATC_C Cuts at: 2691 2869 3236 2691 Size: 178 367 4994 Fragments arranged by size: 4994 367 178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2143 2319 2860 4315 618 Size: 325 217 627 356 176 541 1455 1842 Fragments arranged by size: 1842 1455 627 541 356 325 217 176 BanII G_rGCy'C Cuts at: 1391 2482 2620 2858 1391 Size: 1091 138 238 4072 Fragments arranged by size: 4072 1091 238 138 BbeI G_GCGC'C Cuts at: 2323 2323 Size: 5539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4568 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3730 3764 1903 Size: 34 1793 34 3678 Fragments arranged by size: 3678 1793 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3431 4641 3431 Size: 1210 4329 BclI T'GATC_A Cuts at: 1548 1548 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4150 136 Size: 107 122 71 631 605 2478 1525 Fragments arranged by size: 2478 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5534 5534 Size: 5539 BlpI GC'TnA_GC Cuts at: 2382 2956 2382 Size: 574 4965 Bme1580I G_kGCm'C Cuts at: 1790 2482 2620 3600 4756 1790 Size: 692 138 980 1156 2573 Fragments arranged by size: 2573 1156 980 692 138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5539 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 4228 442 Size: 416 53 83 399 24 1853 958 1753 Fragments arranged by size: 1853 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2723 4199 1239 Size: 1013 471 1476 2579 Fragments arranged by size: 2579 1476 1013 471 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3664 4529 4827 3664 Size: 865 298 4376 Fragments arranged by size: 4376 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4202 882 Size: 617 2703 2219 Fragments arranged by size: 2703 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3330 493 Size: 711 2126 2702 Fragments arranged by size: 2702 2126 711 BsaBI GATnn'nnATC Cuts at: 5533 5533 Size: 5539 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2320 3346 3728 4363 Size: 53 83 186 936 787 1026 382 635 Cuts at: 4363 275 Size: 1451 Fragments arranged by size: 1451 1026 936 787 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2796 3972 4370 4644 4791 5202 2796 Size: 1176 398 274 147 411 3133 Fragments arranged by size: 3133 1176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5314 Fragments arranged by size: 5314 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2048 2051 2066 2078 2081 2084 2300 Size: 847 15 3 15 12 3 3 216 Cuts at: 2300 2408 1186 Size: 108 4317 Fragments arranged by size: 4317 847 216 108 15 15 12 3 3 3 BseYI C'CCAG_C Cuts at: 4742 5238 4742 Size: 496 5043 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4966 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2989 3750 4778 1540 Size: 275 186 988 761 1028 2301 Fragments arranged by size: 2301 1028 988 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3600 3685 4756 3600 Size: 85 1071 4383 Fragments arranged by size: 4383 1071 85 BsmI GAATG_Cn' Cuts at: 3050 3143 3050 Size: 93 5446 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2482 2620 2858 3600 3685 4756 1391 Size: 399 692 138 238 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 692 399 238 138 85 BspEI T'CCGG_A Cuts at: 2796 5202 2796 Size: 2406 3133 BspHI T'CATG_A Cuts at: 3428 5158 3428 Size: 1730 3809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2235 2894 844 Size: 1391 659 3489 Fragments arranged by size: 3489 1391 659 BsrBI CCG'CTC Cuts at: 1394 1823 2317 3426 1394 Size: 429 494 1109 3507 Fragments arranged by size: 3507 1109 494 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4034 4208 65 Size: 1183 2786 174 1396 Fragments arranged by size: 2786 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2095 2322 4183 4370 1700 Size: 110 285 227 1861 187 2869 Fragments arranged by size: 2869 1861 285 227 187 110 BsrGI T'GTAC_A Cuts at: 96 2254 2766 96 Size: 2158 512 2869 Fragments arranged by size: 2869 2158 512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5539 BssSI C'ACGA_G Cuts at: 3599 4611 3599 Size: 1012 4527 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5539 BstBI TT'CG_AA Cuts at: 4343 4343 Size: 5539 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5539 BstYI r'GATC_y Cuts at: 1972 2166 2691 2869 3236 3621 3638 5090 5176 Size: 194 525 178 367 385 17 1452 86 Cuts at: 5176 5188 5534 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 525 385 367 346 194 178 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2139 513 Size: 552 1074 3913 Fragments arranged by size: 3913 1074 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4800 Fragments arranged by size: 4800 683 56 BtsI GCAGTG_nn' Cuts at: 3055 3849 3869 3055 Size: 794 20 4725 Fragments arranged by size: 4725 794 20 ClaI AT'CG_AT Cuts at: 1554 2250 1554 Size: 696 4843 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5504 DraI TTT'AAA Cuts at: 2843 2843 Size: 5539 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4546 809 Size: 507 3230 1802 Fragments arranged by size: 3230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504 Size: 54 1425 50 275 18 1156 889 1629 Cuts at: 5504 8 Size: 43 Fragments arranged by size: 1629 1425 1156 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2986 1537 Size: 275 1174 4090 Fragments arranged by size: 4090 1174 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 2079 2106 2277 2406 2412 3469 1353 Size: 690 36 27 171 129 6 1057 3423 Fragments arranged by size: 3423 1057 690 171 129 36 27 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2405 4126 4500 4646 387 Size: 1064 138 816 1721 374 146 1280 Fragments arranged by size: 1721 1280 1064 816 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2335 2723 3602 4199 4986 5254 Size: 393 620 83 388 879 597 787 268 Cuts at: 5254 1239 Size: 1524 Fragments arranged by size: 1524 879 787 620 597 393 388 268 83 EcoNI CCTnn'n_nnAGG Cuts at: 2501 2501 Size: 5539 EcoO109I rG'GnC_Cy Cuts at: 2478 2478 Size: 5539 EcoRI G'AATT_C Cuts at: 2848 3226 2848 Size: 378 5161 FspI TGC'GCA Cuts at: 2555 4045 2555 Size: 1490 4049 FspAI rTGC'GCAy Cuts at: 2555 2555 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 2323 2350 4686 1678 Size: 645 27 2336 2531 Fragments arranged by size: 2531 2336 645 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4180 4212 4254 4286 1373 Size: 32 135 32 2608 32 42 32 2626 Fragments arranged by size: 2626 2608 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 2883 3129 669 Size: 988 281 250 695 246 3079 Fragments arranged by size: 3079 988 695 281 250 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5539 HpaI GTT'AAC Cuts at: 3129 3129 Size: 5539 KasI G'GCGC_C Cuts at: 2319 2319 Size: 5539 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2493 2664 2803 4653 4837 1305 Size: 684 504 171 139 1850 184 2007 Fragments arranged by size: 2007 1850 684 504 184 171 139 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3717 Fragments arranged by size: 3717 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3497 3856 4015 518 Size: 648 2331 359 159 2042 Fragments arranged by size: 2331 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2269 2701 2832 2955 3632 4780 5025 1606 Size: 663 432 131 123 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 663 432 245 131 123 NarI GG'CG_CC Cuts at: 2320 2320 Size: 5539 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4987 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2897 4442 1132 Size: 97 1668 1545 2229 Fragments arranged by size: 2229 1668 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5539 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 2701 1108 Size: 1593 3946 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 3640 5121 5153 3608 Size: 32 1481 32 3994 Fragments arranged by size: 3994 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5539 PsiI TTA'TAA Cuts at: 3109 3109 Size: 5539 PspOMI G'GGCC_C Cuts at: 2478 2616 2478 Size: 138 5401 PstI C_TGCA'G Cuts at: 830 2241 2891 830 Size: 1411 650 3478 Fragments arranged by size: 3478 1411 650 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 PvuII CAG'CTG Cuts at: 2701 2701 Size: 5539 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SapI GCTCTTCn'nnn_ Cuts at: 2079 2079 Size: 5539 SbfI CC_TGCA'GG Cuts at: 2891 2891 Size: 5539 ScaI AGT'ACT Cuts at: 3787 5499 3787 Size: 1712 3827 SexAI A'CCwGG_T Cuts at: 2496 2496 Size: 5539 SfcI C'TryA_G Cuts at: 826 1045 2237 2887 4022 4703 4894 826 Size: 219 1192 650 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 650 219 191 SfoI GGC'GCC Cuts at: 2321 2321 Size: 5539 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5539 SmaI CCC'GGG Cuts at: 1872 2866 1872 Size: 994 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3643 4544 4806 820 Size: 197 926 1700 901 262 1553 Fragments arranged by size: 1700 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3330 493 Size: 2837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2897 1132 Size: 1765 3774 SspI AAT'ATT Cuts at: 5 52 3463 5 Size: 47 3411 2081 Fragments arranged by size: 3411 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5539 StyI C'CwwG_G Cuts at: 513 1065 2978 513 Size: 552 1913 3074 Fragments arranged by size: 3074 1913 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3566 3583 3736 3921 4366 325 Size: 1255 1986 17 153 185 445 1498 Fragments arranged by size: 1986 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2254 2766 3785 Size: 274 80 33 51 1443 277 512 1019 Cuts at: 3785 5497 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1019 512 277 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5499 5531 5499 Size: 32 5507 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5539 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5539 XmaI C'CCGG_G Cuts at: 1870 2864 1870 Size: 994 4545 XmnI GAAnn'nnTTC Cuts at: 3668 3668 Size: 5539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3347 276 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI