################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsgI BsiEI BsiHKAI BsmFI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI Ksp632I MaeI MamI MboII MfeI MluI MseI MslI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 38 GGTACC G^GTACC AccI 706 GTCTAC GT^[AC][GT]AC AccII 429 CGCG CG^CG AccIII 395 TCCGGA T^CCGGA AcsI 724 AAATTC [GA]^AATT[CT] AcyI 172 GGCGTC G[GA]^CG[CT]C AflIII 506 ACGTGT A^C[GA][CT]GT AlwI 61 GGATC GGATC(4/5) ApaI 625 GGGCCC GGGCC^C ApoI 724 AAATTC [GA]^AATT[CT] Asp718I 38 GGTACC G^GTACC AvaI 168 CTCGGG C^[CT]CG[GA]G BanII 625 GGGCCC G[GA]GC[CT]^C BglI 686 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 172 GGCGTC G[GA]^CG[CT]C BsaWI 395 TCCGGA [AT]^CCGG[AT] BseAI 395 TCCGGA T^CCGGA BseRI 523 GAGGAG GAGGAG(10/8) BsiWI 306 CGTACG C^GTACG BsmAI 70 GTCTC GTCTC(1/5) BsmBI 69 CGTCTC CGTCTC(1/5) BsmI 479 GAATGC GAATGC(1/-1) BsoBI 168 CTCGGG C^[CT]CG[GA]G BspEI 395 TCCGGA T^CCGGA BsrDI 408 CATTGC GCAATG(2/0) BsrFI 577 GCCGGC [GA]^CCGG[CT] BstUI 429 CGCG CG^CG BstXI 233 CCATCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 61 GGATCT [GA]^GATC[CT] CfoI 575 GCGC GCG^C Cfr10I 577 GCCGGC [GA]^CCGG[CT] DraIII 729 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 428 CCGCGG C^C[GA][CT]GG EcoT14I 48 CCTTGG C^C[AT][AT]GG HgaI 173 GCGTC GACGC(5/10) HhaI 575 GCGC GCG^C Hin1I 172 GGCGTC G[GA]^CG[CT]C HinP1I 575 GCGC G^CGC HphI 613 GGTGA GGTGA(8/7) KpnI 38 GGTACC GGTAC^C KspI 428 CCGCGG CCGC^GG MaeII 506 ACGT A^CGT MaeIII 614 GTGAC ^GT[ACGT]AC MflI 61 GGATCT [GA]^GATC[CT] MroI 395 TCCGGA T^CCGGA MvnI 429 CGCG CG^CG NaeI 577 GCCGGC GCC^GGC NgoMIV 577 GCCGGC G^CCGGC NspI 566 GCATGT [GA]CATG^[CT] PflMI 282 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 428 CCGCGG CCGC^GG SgrAI 576 CGCCGGCG C[GA]^CCGG[CT]G SmlI 256 CTTGAG C^T[CT][GA]AG StyI 48 CCTTGG C^C[AT][AT]GG TfiI 370 GAATC G^A[AT]TC Tsp45I 614 GTGAC ^GT[GC]AC Van91I 282 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 61 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 79 AGCT AG^CT 539 AGCT BalI 2 TGGCCA TGG^CCA 684 TGGCCA BanI 38 GGTACC G^G[CT][GA]CC 311 GGCACC BmyI 420 GTGCCC G[AGT]GC[ACT]^C 625 GGGCCC Bsp1286I 420 GTGCCC G[AGT]GC[ACT]^C 625 GGGCCC BsrI 141 ACTGG ACTGG(1/-1) 758 CCAGT BstAPI 189 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 566 GCATGTTCTGC DdeI 521 CTGAG C^T[ACGT]AG 731 CTGAG DpnII 62 GATC ^GATC 633 GATC EaeI 2 TGGCCA [CT]^GGCC[GA] 684 TGGCCA HapII 396 CCGG C^CGG 578 CCGG HpaII 396 CCGG C^CGG 578 CCGG MboI 62 GATC ^GATC 633 GATC MluNI 2 TGGCCA TGG^CCA 684 TGGCCA MscI 2 TGGCCA TGG^CCA 684 TGGCCA MspA1I 428 CCGCGG C[AC]G^C[GT]G 550 CCGCTG MspI 396 CCGG C^CGG 578 CCGG NdeII 62 GATC ^GATC 633 GATC Sau3AI 62 GATC ^GATC 633 GATC SfaNI 183 GCATC GCATC(5/9) 401 GCATC Tsp509I 158 AATT ^AATT 725 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AfaI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI ItaI MnlI MvaI MwoI NlaIII NlaIV PleI PstI RsaI Sau96I ScrFI SfcI TseI TspRI