################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AgeI AhdI Alw44I AlwI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI Hin1I HindIII HinfI HpaI KasI KpnI KspI MamI MfeI MflI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 532 AAATTC [GA]^AATT[CT] AflIII 58 ACACGT A^C[GA][CT]GT Aor51HI 787 AGCGCT AGC^GCT ApoI 532 AAATTC [GA]^AATT[CT] AspHI 516 GTGCTC G[AT]GC[AT]^C AvaI 1061 CCCGGG C^[CT]CG[GA]G AviII 522 TGCGCA TGC^GCA BalI 654 TGGCCA TGG^CCA BanI 697 GGCACC G^G[CT][GA]CC BbrPI 59 CACGTG CAC^GTG BbvCI 366 CCTCAGC CCTCAGC(-5/-2) BfaI 899 CTAG C^TAG BglI 759 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 59 CACGTG [CT]AC^GT[GA] BsgI 463 GTGCAG GTGCAG(16/14) BsiHKAI 516 GTGCTC G[AT]GC[AT]^C BsoBI 1061 CCCGGG C^[CT]CG[GA]G BspMI 700 ACCTGC ACCTGC(4/8) BsrFI 755 ACCGGC [GA]^CCGG[CT] Cfr10I 755 ACCGGC [GA]^CCGG[CT] DpnII 64 GATC ^GATC DsaI 1047 CCGTGG C^C[GA][CT]GG EarI 90 CTCTTC CTCTTC(1/4) Eco47III 787 AGCGCT AGC^GCT EcoNI 102 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 908 CCTTGG C^C[AT][AT]GG FspI 522 TGCGCA TGC^GCA HaeII 787 AGCGCT [GA]GCGC^[CT] HgaI 154 GCGTC GACGC(5/10) HincII 919 GTCAAC GT[CT]^[GA]AC HindII 919 GTCAAC GT[CT]^[GA]AC Ksp632I 90 CTCTTC CTCTTC(1/4) MaeI 899 CTAG C^TAG MboI 64 GATC ^GATC MluNI 654 TGGCCA TGG^CCA MscI 654 TGGCCA TGG^CCA MseI 815 TTAA T^TAA MspA1I 466 CAGCGG C[AC]G^C[GT]G NdeII 64 GATC ^GATC NheI 898 GCTAGC G^CTAGC PmaCI 59 CACGTG CAC^GTG PmlI 59 CACGTG CAC^GTG PstI 30 CTGCAG CTGCA^G Sau3AI 64 GATC ^GATC SfcI 30 CTGCAG C^T[GA][CT]AG SmaI 1061 CCCGGG CCC^GGG SmlI 856 CTCAAG C^T[CT][GA]AG StyI 908 CCTTGG C^C[AT][AT]GG Tru9I 815 TTAA T^TAA Tsp45I 35 GTGAC ^GT[GC]AC Tsp509I 533 AATT ^AATT XmaCI 1061 CCCGGG C^CCGGG XmaI 1061 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccIII 94 TCCGGA T^CCGGA 536 TCCGGA AfaI 297 GTAC GT^AC 563 GTAC AlwNI 24 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 381 CAGTAACTG ApaI 443 GGGCCC GGGCC^C 679 GGGCCC BanII 443 GGGCCC G[GA]GC[CT]^C 679 GGGCCC BpmI 309 CTCCAG CTGGAG(16/14) 892 CTCCAG BsaI 9 GGTCTC GGTCTC(1/5) 225 GGTCTC BsaWI 94 TCCGGA [AT]^CCGG[AT] 536 TCCGGA BseAI 94 TCCGGA T^CCGGA 536 TCCGGA BspEI 94 TCCGGA T^CCGGA 536 TCCGGA BsrDI 288 CATTGC GCAATG(2/0) 179 GCAATG BsrI 380 CCAGT ACTGG(1/-1) 984 CCAGT EaeI 503 TGGCCG [CT]^GGCC[GA] 654 TGGCCA FauI 468 GCGGG CCCGC(4/6) 732 GCGGG MaeIII 35 GTGAC ^GT[ACGT]AC 383 GTAAC MroI 94 TCCGGA T^CCGGA 536 TCCGGA PflMI 311 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 545 CCAGAAAATGG PpuMI 50 GGGTCCT [GA]G^G[AT]CC[CT] 118 GGGTCCC RsaI 297 GTAC GT^AC 563 GTAC Van91I 311 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 545 CCAGAAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AvaII BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI