(Circular) (Six-base) MAPSORT of: pFN21AE9411.seq Check: 7247 from: 1 to: 5773 pFN21AE9411 5773 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2714 2714 Size: 5773 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3836 5220 5488 1632 Size: 2204 1384 268 1917 Fragments arranged by size: 2204 1917 1384 268 AfeI AGC'GCT Cuts at: 2794 2794 Size: 5773 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 2063 4672 5649 1203 Size: 860 2609 977 1327 Fragments arranged by size: 2609 1327 977 860 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 2363 1166 Size: 1197 4576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 2034 2391 3516 5088 2034 Size: 357 1125 1572 2719 Fragments arranged by size: 2719 1572 1125 357 ApaI G_GGCC'C Cuts at: 2452 2688 2452 Size: 236 5537 ApaLI G'TGCA_C Cuts at: 3830 4986 3830 Size: 1156 4617 ApoI r'AATT_y Cuts at: 1493 2537 3082 3308 3460 5569 1493 Size: 1044 545 226 152 2109 1697 Fragments arranged by size: 2109 1697 1044 545 226 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 3066 3098 1870 Size: 73 1123 32 4545 Fragments arranged by size: 4545 1123 73 32 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2702 3094 4549 618 Size: 325 217 627 915 392 1455 1842 Fragments arranged by size: 1842 1455 915 627 392 325 217 BanII G_rGCy'C Cuts at: 1391 2452 2688 3092 1391 Size: 1061 236 404 4072 Fragments arranged by size: 4072 1061 404 236 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2203 2395 2947 928 Size: 971 304 192 552 3754 Fragments arranged by size: 3754 971 552 304 192 BbvCI CC'TCA_GC Cuts at: 2372 2372 Size: 5773 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 1548 Size: 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2770 4384 136 Size: 107 122 71 631 605 1098 1614 1525 Fragments arranged by size: 1614 1525 1098 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 2452 2688 3834 4990 1790 Size: 662 236 1146 1156 2573 Fragments arranged by size: 2573 1156 1146 662 236 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2907 1056 Size: 1851 3922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2299 2882 4433 1239 Size: 1060 583 1551 2579 Fragments arranged by size: 2579 1551 1060 583 Bpu10I CC'TnA_GC Cuts at: 2372 2689 2372 Size: 317 5456 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2846 3898 4763 5061 2846 Size: 1052 865 298 3558 Fragments arranged by size: 3558 1052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2020 2236 4436 882 Size: 617 521 216 2200 2219 Fragments arranged by size: 2219 2200 617 521 216 BsaAI yAC'GTr Cuts at: 493 1204 2066 3564 493 Size: 711 862 1498 2702 Fragments arranged by size: 2702 1498 862 711 BsaBI GATnn'nnATC Cuts at: 5767 5767 Size: 5773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3580 3962 4597 275 Size: 53 83 186 936 2047 382 635 1451 Fragments arranged by size: 2047 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2099 2541 4206 4604 4878 5025 5436 2099 Size: 442 1665 398 274 147 411 2436 Fragments arranged by size: 2436 1665 442 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 3023 3053 1178 Size: 30 165 30 705 30 885 30 3898 Fragments arranged by size: 3898 885 705 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2318 3041 3044 1186 Size: 943 189 723 3 3915 Fragments arranged by size: 3915 943 723 189 3 BseYI C'CCAG_C Cuts at: 2179 2671 2745 4976 5472 2179 Size: 492 74 2231 496 2480 Fragments arranged by size: 2480 2231 496 492 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2489 1163 Size: 573 753 4447 Fragments arranged by size: 4447 753 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2525 3834 3919 4990 2525 Size: 1309 85 1071 3308 Fragments arranged by size: 3308 1309 1071 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2452 2525 2688 3092 3834 3919 4990 Size: 399 662 73 163 404 742 85 1071 Cuts at: 4990 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 662 404 399 163 85 73 BspEI T'CCGG_A Cuts at: 2099 2541 5436 2099 Size: 442 2895 2436 Fragments arranged by size: 2895 2436 442 BspHI T'CATG_A Cuts at: 3662 5392 3662 Size: 1730 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2714 3128 844 Size: 1870 414 3489 Fragments arranged by size: 3489 1870 414 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2191 2292 4268 4442 65 Size: 1183 943 101 1976 174 1396 Fragments arranged by size: 1976 1396 1183 943 174 101 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 4417 4604 1700 Size: 110 950 1657 187 2869 Fragments arranged by size: 2869 1657 950 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5773 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5773 BstYI r'GATC_y Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768 Size: 1131 367 385 17 1452 86 12 346 Cuts at: 5768 1972 Size: 1977 Fragments arranged by size: 1977 1452 1131 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3052 513 Size: 552 1987 3234 Fragments arranged by size: 3234 1987 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5034 Fragments arranged by size: 5034 683 56 BtsI GCAGTG_nn' Cuts at: 2044 2327 2572 3289 4083 4103 2044 Size: 283 245 717 794 20 3714 Fragments arranged by size: 3714 794 717 283 245 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2508 2659 3220 Size: 54 1425 50 275 18 678 151 561 Cuts at: 3220 4109 5738 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 678 561 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2101 3703 1353 Size: 748 1602 3423 Fragments arranged by size: 3423 1602 748 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2120 4360 4734 4880 387 Size: 1064 138 531 2240 374 146 1280 Fragments arranged by size: 2240 1280 1064 531 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 2882 3836 4433 5220 5488 1239 Size: 393 667 583 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 667 597 583 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2111 2111 Size: 5773 EcoO109I rG'GnC_Cy Cuts at: 2056 2124 2449 2684 2685 2056 Size: 68 325 235 1 5144 Fragments arranged by size: 5144 325 235 68 1 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 FspI TGC'GCA Cuts at: 2529 4279 2529 Size: 1750 4023 HaeII r_GCGC'y Cuts at: 1678 2796 4920 1678 Size: 1118 2124 2531 Fragments arranged by size: 2531 2124 1118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2085 2108 2140 3023 Size: 32 135 32 481 32 23 32 883 Cuts at: 3023 3055 4414 4446 4488 4520 1373 Size: 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 883 481 135 42 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2926 3117 3363 669 Size: 988 281 988 191 246 3079 Fragments arranged by size: 3079 988 988 281 246 191 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2152 4887 5071 1305 Size: 847 2735 184 2007 Fragments arranged by size: 2735 2007 847 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2661 10 Size: 54 1425 343 829 3122 Fragments arranged by size: 3122 1425 829 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2363 2411 3731 4090 4249 518 Size: 648 1131 66 48 1320 359 159 2042 Fragments arranged by size: 2042 1320 1131 648 359 159 66 48 MspA1I CmG'CkG Cuts at: 1606 2473 3189 3866 5014 5259 1606 Size: 867 716 677 1148 245 2120 Fragments arranged by size: 2120 1148 867 716 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5221 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NheI G'CTAG_C Cuts at: 1052 2903 1052 Size: 1851 3922 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 3131 4676 1132 Size: 97 1902 1545 2229 Fragments arranged by size: 2229 1902 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5773 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 2322 2556 1108 Size: 1214 234 4325 Fragments arranged by size: 4325 1214 234 PfoI T'CCnGG_A Cuts at: 2081 2081 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PmlI CAC'GTG Cuts at: 2066 2066 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2124 2056 Size: 68 5705 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5773 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PspOMI G'GGCC_C Cuts at: 2448 2684 2448 Size: 236 5537 PstI C_TGCA'G Cuts at: 830 2039 3125 830 Size: 1209 1086 3478 Fragments arranged by size: 3478 1209 1086 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SanDI GG'GwC_CC Cuts at: 2124 2124 Size: 5773 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 2035 3121 4256 4937 5128 826 Size: 219 990 1086 1135 681 191 1471 Fragments arranged by size: 1471 1135 1086 990 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 3068 3100 1872 Size: 1196 32 4545 Fragments arranged by size: 4545 1196 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 2861 3877 4778 5040 820 Size: 197 926 918 1016 901 262 1553 Fragments arranged by size: 1553 1016 926 918 901 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 2913 3212 513 Size: 552 1848 299 3074 Fragments arranged by size: 3074 1848 552 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3800 3817 3970 4155 4600 325 Size: 1255 2220 17 153 185 445 1498 Fragments arranged by size: 2220 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 4019 5731 Size: 274 80 33 51 1443 324 1718 1712 Cuts at: 5731 96 Size: 138 Fragments arranged by size: 1718 1712 1443 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5733 5765 5733 Size: 32 5741 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 3066 3098 1870 Size: 1196 32 4545 Fragments arranged by size: 4545 1196 32 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI