(Linear) (Six-base) MAPSORT of: KIEE1854.seq Check: 1803 from: 1 to: 1059 KIEE1854.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AatII G_ACGT'C Cuts at: 0 230 1059 Size: 230 829 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 1059 Size: 166 893 AflIII A'CryG_T Cuts at: 0 599 614 1059 Size: 599 15 445 Fragments arranged by size: 599 445 15 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 285 317 1059 Size: 285 32 742 Fragments arranged by size: 742 285 32 ApaI G_GGCC'C Cuts at: 0 279 1059 Size: 279 780 ApaLI G'TGCA_C Cuts at: 0 787 1059 Size: 787 272 AvaI C'yCGr_G Cuts at: 0 234 485 859 1059 Size: 234 251 374 200 Fragments arranged by size: 374 251 234 200 BanI G'GyrC_C Cuts at: 0 197 238 501 943 1059 Size: 197 41 263 442 116 Fragments arranged by size: 442 263 197 116 41 BanII G_rGCy'C Cuts at: 0 279 398 650 1059 Size: 279 119 252 409 Fragments arranged by size: 409 279 252 119 BbeI G_GCGC'C Cuts at: 0 505 1059 Size: 505 554 BbsI GAAGACnn'nnnn_ Cuts at: 0 740 928 1059 Size: 740 188 131 Fragments arranged by size: 740 188 131 BbvCI CC'TCA_GC Cuts at: 0 77 1059 Size: 77 982 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 270 304 1059 Size: 270 34 755 Fragments arranged by size: 755 270 34 BglI GCCn_nnn'nGGC Cuts at: 0 799 1059 Size: 799 260 BglII A'GATC_T Cuts at: 0 935 1059 Size: 935 124 Bme1580I G_kGCm'C Cuts at: 0 241 279 791 1059 Size: 241 38 512 268 Fragments arranged by size: 512 268 241 38 BmrI ACTGGGnnnn_n' Cuts at: 0 394 1059 Size: 394 665 Bpu10I CC'TnA_GC Cuts at: 0 77 1059 Size: 77 982 BsaAI yAC'GTr Cuts at: 0 357 786 1059 Size: 357 429 273 Fragments arranged by size: 429 357 273 BsaHI Gr'CG_yC Cuts at: 0 227 502 1059 Size: 227 275 557 Fragments arranged by size: 557 275 227 BsaWI w'CCGG_w Cuts at: 0 1049 1059 Size: 1049 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 94 124 163 193 586 616 1059 Size: 94 30 39 30 393 30 443 Fragments arranged by size: 443 393 94 39 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 151 400 946 1059 Size: 151 249 546 113 Fragments arranged by size: 546 249 151 113 BsiHKAI G_wGCw'C Cuts at: 0 398 479 791 1059 Size: 398 81 312 268 Fragments arranged by size: 398 312 268 81 Bsp1286I G_dGCh'C Cuts at: 0 241 279 398 479 650 791 1059 Size: 241 38 119 81 171 141 268 Fragments arranged by size: 268 241 171 141 119 81 38 BsrFI r'CCGG_y Cuts at: 0 194 241 560 841 1059 Size: 194 47 319 281 218 Fragments arranged by size: 319 281 218 194 47 BssSI C'ACGA_G Cuts at: 0 133 1059 Size: 133 926 BstAPI GCAn_nnn'nTGC Cuts at: 0 251 1059 Size: 251 808 BstEII G'GTnAC_C Cuts at: 0 360 681 1059 Size: 360 321 378 Fragments arranged by size: 378 360 321 BstXI CCAn_nnnn'nTGG Cuts at: 0 678 1002 1059 Size: 678 324 57 Fragments arranged by size: 678 324 57 BstYI r'GATC_y Cuts at: 0 581 935 1059 Size: 581 354 124 Fragments arranged by size: 581 354 124 BtsI GCAGTG_nn' Cuts at: 0 66 1059 Size: 66 993 DraIII CAC_nnn'GTG Cuts at: 0 360 1059 Size: 360 699 EaeI y'GGCC_r Cuts at: 0 45 548 635 1059 Size: 45 503 87 424 Fragments arranged by size: 503 424 87 45 EarI CTCTTCn'nnn_ Cuts at: 0 208 454 1059 Size: 208 246 605 Fragments arranged by size: 605 246 208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 67 1059 Size: 67 992 EcoICRI GAG'CTC Cuts at: 0 396 1059 Size: 396 663 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 1059 Size: 166 893 EcoNI CCTnn'n_nnAGG Cuts at: 0 205 700 1059 Size: 205 495 359 Fragments arranged by size: 495 359 205 EcoO109I rG'GnC_Cy Cuts at: 0 275 981 1059 Size: 275 706 78 Fragments arranged by size: 706 275 78 FspI TGC'GCA Cuts at: 0 160 1059 Size: 160 899 FspAI rTGC'GCAy Cuts at: 0 160 1059 Size: 160 899 HaeII r_GCGC'y Cuts at: 0 505 1059 Size: 505 554 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 73 105 217 249 1059 Size: 73 32 112 32 810 Fragments arranged by size: 810 112 73 32 32 KasI G'GCGC_C Cuts at: 0 501 1059 Size: 501 558 MscI TGG'CCA Cuts at: 0 47 637 1059 Size: 47 590 422 Fragments arranged by size: 590 422 47 MslI CAynn'nnrTG Cuts at: 0 34 952 1059 Size: 34 918 107 Fragments arranged by size: 918 107 34 MspA1I CmG'CkG Cuts at: 0 678 1020 1059 Size: 678 342 39 Fragments arranged by size: 678 342 39 NarI GG'CG_CC Cuts at: 0 502 1059 Size: 502 557 NspI r_CATG'y Cuts at: 0 258 618 957 1059 Size: 258 360 339 102 Fragments arranged by size: 360 339 258 102 PciI A'CATG_T Cuts at: 0 614 1059 Size: 614 445 PmlI CAC'GTG Cuts at: 0 357 1059 Size: 357 702 PpuMI rG'GwC_Cy Cuts at: 0 981 1059 Size: 981 78 PspOMI G'GGCC_C Cuts at: 0 275 1059 Size: 275 784 PvuII CAG'CTG Cuts at: 0 678 1020 1059 Size: 678 342 39 Fragments arranged by size: 678 342 39 SacI G_AGCT'C Cuts at: 0 398 1059 Size: 398 661 SfoI GGC'GCC Cuts at: 0 503 1059 Size: 503 556 SmaI CCC'GGG Cuts at: 0 487 1059 Size: 487 572 SphI G_CATG'C Cuts at: 0 957 1059 Size: 957 102 StyI C'CwwG_G Cuts at: 0 266 1059 Size: 266 793 TatI w'GTAC_w Cuts at: 0 566 1059 Size: 566 493 XmaI C'CCGG_G Cuts at: 0 485 1059 Size: 485 574 ZraI GAC'GTC Cuts at: 0 228 1059 Size: 228 831 Enzymes that do cut: AatII AcuI AflIII AloI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmrI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BsrFI BssSI BstAPI BstEII BstXI BstYI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I KasI MscI MslI MspA1I NarI NspI PciI PmlI PpuMI PspOMI PvuII SacI SfoI SmaI SphI StyI TatI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI