################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII ClaI DpnII DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MroI MseI MunI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI SacI SacII SalI Sau3AI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 426 GTCTAC GT^[AC][GT]AC AccII 103 CGCG CG^CG AcyI 76 GGCGCC G[GA]^CG[CT]C AocI 930 CCTGAGG CC^T[ACGT]AGG AspI 988 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 409 CTCGGG C^[CT]CG[GA]G AvaII 51 GGACC G^G[AT]CC BbeI 76 GGCGCC GGCGC^C BpmI 579 CTCCAG CTGGAG(16/14) BsaHI 76 GGCGCC G[GA]^CG[CT]C BsaI 993 GGTCTC GGTCTC(1/5) BsaWI 608 TCCGGT [AT]^CCGG[AT] BseRI 576 CTCCTC GAGGAG(10/8) BsiEI 694 CGGCCG CG[GA][CT]^CG BsmFI 939 GTCCC GGGAC(10/14) BsmI 591 GAATGC GAATGC(1/-1) BsoBI 409 CTCGGG C^[CT]CG[GA]G BsrBI 112 CCGCTC CCGCTC(-3/-3) BstUI 103 CGCG CG^CG Bsu36I 930 CCTGAGG CC^T[ACGT]AGG CpoI 50 CGGACCG CG^G[AT]CCG EagI 694 CGGCCG C^GGCCG EarI 868 CTCTTC CTCTTC(1/4) EclXI 694 CGGCCG C^GGCCG Eco52I 694 CGGCCG C^GGCCG Eco81I 930 CCTGAGG CC^T[ACGT]AGG EcoT14I 161 CCATGG C^C[AT][AT]GG Hin1I 76 GGCGCC G[GA]^CG[CT]C KasI 76 GGCGCC G^GCGCC Ksp632I 868 CTCTTC CTCTTC(1/4) MslI 711 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 103 CGCG CG^CG NarI 76 GGCGCC GG^CGCC NcoI 161 CCATGG C^CATGG PflFI 988 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 937 GAGTC GAGTC(4/5) PstI 550 CTGCAG CTGCA^G RsrII 50 CGGACCG CG^G[AT]CCG SapI 867 GCTCTTC GCTCTTC(1/4) SfcI 550 CTGCAG C^T[GA][CT]AG SfoI 76 GGCGCC GGC^GCC StyI 161 CCATGG C^C[AT][AT]GG TaqI 92 TCGA T^CGA TfiI 10 GATTC G^A[AT]TC Tth111I 988 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 92 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 532 GAGCAC G[AT]GC[AT]^C 763 GTGCTC BcnI 151 CCGGG CC^[GC]GG 545 CCCGG BsiHKAI 532 GAGCAC G[AT]GC[AT]^C 763 GTGCTC BsmAI 255 GTCTC GTCTC(1/5) 994 GTCTC BspMI 749 ACCTGC ACCTGC(4/8) 453 GCAGGT BsrDI 279 CATTGC GCAATG(2/0) 155 GCAATG BstXI 131 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 710 CCATTGTGGTGG DdeI 382 CTCAG C^T[ACGT]AG 931 CTGAG HaeII 76 GGCGCC [GA]GCGC^[CT] 324 GGCGCT HgaI 686 GACGC GACGC(5/10) 362 GCGTC HinfI 10 GATTC G^A[ACGT]TC 937 GAGTC HphI 597 GGTGA GGTGA(8/7) 494 TCACC MboII 134 TCTTC GAAGA(8/7) 869 TCTTC NaeI 518 GCCGGC GCC^GGC 692 GCCGGC NciI 151 CCGGG CC^[GC]GG 545 CCCGG NgoMIV 518 GCCGGC G^CCGGC 692 GCCGGC PflMI 747 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 984 CCATGACCTGG SexAI 211 ACCTGGT A^CC[AT]GGT 989 ACCTGGT Van91I 747 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 984 CCATGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI ApaI BalI BanI BanII BbvI BglI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MluNI MnlI MscI MspA1I MspI MvaI MwoI NlaIII NlaIV PvuII Sau96I ScrFI TseI Tsp45I TspRI XcmI